(Q28142221)

https://doi.org/10.1016%2FS0065-3233%2801%2955002-0

27 March 2017

0 references

M Ostermeier

1

0 references

S J Benkovic

series ordinal

2

0 references

Marc Ostermeier

series ordinal

1

1 reference

https://doi.org/10.1016%2FS0065-3233%2801%2955002-0

27 March 2017

Stephen J. Benkovic

series ordinal

2

1 reference

https://doi.org/10.1016%2FS0065-3233%2801%2955002-0

27 March 2017

language of work or name

English

0 references

publication date

2000

0 references

2001

1 reference

https://doi.org/10.1016%2FS0065-3233%2801%2955002-0

Advances in Protein Chemistry

27 March 2017

published in

1 reference

https://doi.org/10.1016%2FS0065-3233%2801%2955002-0

27 March 2017

volume

55

0 references

page(s)

29-77

1 reference

https://doi.org/10.1016%2FS0065-3233%2801%2955002-0

Directed evolution of biocatalysts

27 March 2017

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Recombinant protein expression in Escherichia coli

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In situ behavior of the pyrimidine pathway enzymes in Saccharomyces cerevisiae. 2. Reaction mechanism of aspartate transcarbamylase dissociated from carbamylphosphate synthetase by genetic alteration

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7 January 2021

Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein

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7 January 2021

Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum

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7 January 2021

Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea

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7 January 2021

Engineering of modular polyketide synthases to produce novel polyketides

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7 January 2021

Chimeric restriction enzymes: what is next?

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7 January 2021

Controlling mammalian gene expression with small molecules

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7 January 2021

An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea.

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7 January 2021

Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobacterium extorquens

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7 January 2021

The structure of the quinoprotein alcohol dehydrogenase of Acetobacter aceti modelled on that of methanol dehydrogenase from Methylobacterium extorquens

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7 January 2021

DNA shuffling of a family of genes from diverse species accelerates directed evolution

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7 January 2021

The evolutionary history of the first three enzymes in pyrimidine biosynthesis

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7 January 2021

Origin of genes encoding multi-enzymatic proteins in eukaryotes.

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7 January 2021

Engineering of peptide synthetases. Key role of the thioesterase-like domain for efficient production of recombinant peptides

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7 January 2021

Insertion of foreign random sequences of 120 amino acid residues into an active enzyme

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7 January 2021

Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution

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7 January 2021

Evolutionarily mobile modules in proteins.

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7 January 2021

Progress and variations in two-hybrid and three-hybrid technologies

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7 January 2021

Silent and functional changes in the periplasmic maltose-binding protein of Escherichia coli K12

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7 January 2021

Circular permutation of granulocyte colony-stimulating factor

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7 January 2021

Homotropic Activation via the Subunit Interaction and Allosteric Symmetry Revealed on Analysis of Hybrid Enzymes ofl-Lactate Dehydrogenase

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7 January 2021

Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A

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7 January 2021

Circular and circularly permuted forms of bovine pancreatic trypsin inhibitor

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7 January 2021

A three-step PCR protocol for construction of chimeric proteins

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7 January 2021

A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Crystal structures of reduced and oxidized DsbA: investigation of domain motion and thiolate stabilization

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Comparative modelling of barley-grain aspartic proteinase: A structural rationale for observed hydrolytic specificity

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Function of the major synthetase subdomains of carbamyl-phosphate synthetase

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Pressure-induced dissociation of carbamoyl-phosphate synthetase domains. The catalytically active form is dimeric

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Pentapeptide scanning mutagenesis: random insertion of a variable five amino acid cassette in a target protein

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

The molecular biology of multidomain proteins. Selected examples

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

On the classification and evolution of protein modules

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Circular permutation of polypeptide chains: implications for protein folding and stability

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

The Saccharomyces cerevisiae ADE5,7 protein is homologous to overlapping Drosophila melanogaster Gart polypeptides

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Random circular permutation of DsbA reveals segments that are essential for protein folding and stability

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

The carbamoyl phosphate synthetase-aspartate transcarbamoylase complex of Saccharomyces cerevisiae: molecular and cellular aspects

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7 January 2021

Antibody phage display technology and its applications.

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7 January 2021

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

1 reference

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7 January 2021

Splase: a new class IIS zinc-finger restriction endonuclease with specificity for Sp1 binding sites.

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7 January 2021

Circular Permutations in the Molecular Evolution of DNA Methyltransferases

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7 January 2021

Mouse cDNAs encoding a trifunctional protein of de novo purine synthesis and a related single-domain glycinamide ribonucleotide synthetase

1 reference

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7 January 2021

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

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7 January 2021

Initiation, elongation, and termination strategies in polyketide and polypeptide antibiotic biosynthesis

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7 January 2021

Novel family shuffling methods for the in vitro evolution of enzymes

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7 January 2021

A novel gene-fusing vector: construction of a 5′-GGmCC-specific chimeric methyltransferase, M · BspRI/M · BsuRI

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7 January 2021

Site-specific cleavage of DNA–RNA hybrids by zinc finger/FokI cleavage domain fusions

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7 January 2021

Chimeric restriction enzyme: Gal4 fusion to FokI cleavage domain.

1 reference

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7 January 2021

Production of a novel polyketide through the construction of a hybrid polyketide synthase.

1 reference

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7 January 2021

The evolutionary history of carbamoyltransferases: A complex set of paralogous genes was already present in the last universal common ancestor

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7 January 2021

Selfish operons and speciation by gene transfer

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

C-terminal deletion mutants of the FokI restriction endonuclease.

1 reference

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7 January 2021

Characterization of a recombinant bifunctional enzyme, galactose dehydrogenase/bacterial luciferase, displaying an improved bioluminescence in a three-enzyme system

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Preparation and kinetic characterization of a fusion protein of yeast mitochondrial citrate synthase and malate dehydrogenase.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Circular permutations of natural protein sequences: structural evidence

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Construction of an artificial bifunctional enzyme, beta-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling.

1 reference

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7 January 2021

A new method for constructing linker scanning mutants

1 reference

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7 January 2021

A simple screen for permissive sites in proteins: analysis of Escherichia coli lac permease.

1 reference

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7 January 2021

Engineering broader specificity into an antibiotic-producing polyketide synthase.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Crystal structure of the DsbA protein required for disulphide bond formation in vivo

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Signal sequences: more than just greasy peptides

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7 January 2021

Gene duplications in the structural evolution of chymotrypsin

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7 January 2021

Circular permutation of the granulocyte colony-stimulating factor receptor agonist domain of myelopoietin

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7 January 2021

Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin

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7 January 2021

Design and application of multimodular peptide synthetases

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7 January 2021

Recombination of protein domains facilitated by co-translational folding in eukaryotes.

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7 January 2021

Isolation and physical characterization of random insertions in Staphylococcal nuclease

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7 January 2021

Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins

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7 January 2021

Hybrid enzymes: manipulating enzyme design.

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7 January 2021

A hybrid modular polyketide synthase obtained by domain swapping

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7 January 2021

Transplanting two unique beta-glucanase catalytic activities into one multienzyme, which forms glucose.

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7 January 2021

Incremental truncation as a strategy in the engineering of novel biocatalysts

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7 January 2021

Finding Cinderella's slipper--proteins that fit

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7 January 2021

Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A

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7 January 2021

Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold

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7 January 2021

Swaposins: circular permutations within genes encoding saposin homologues

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7 January 2021

Purification and characterization of carbamoyl-phosphate synthetase from the deep-sea hyperthermophilic archaebacterium Pyrococcus abyssi

1 reference

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7 January 2021

Engineering cyclophilin into a proline-specific endopeptidase

1 reference

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7 January 2021

AraC protein can activate transcription from only one position and when pointed in only one direction

1 reference

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7 January 2021

Recombinant Fv immunotoxins and Fv fragments as novel agents for cancer therapy and diagnosis

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7 January 2021

Functional analysis of an interspecies chimera of acyl carrier proteins indicates a specialized domain for protein recognition.

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7 January 2021

Phage-display technology--finding a needle in a vast molecular haystack

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7 January 2021

Detection in living cells of Ca2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Chemical and biological evolution of nucleotide-binding protein

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Acyltransferase domain substitutions in erythromycin polyketide synthase yield novel erythromycin derivatives

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

1 reference

7 January 2021

Protein fold irregularities that hinder sequence analysis.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Targeted alteration of the substrate specificity of peptide synthetases by rational module swapping

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

EGF domain swap converts a drosophila EGF receptor activator into an inhibitor

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Half of Saccharomyces cerevisiae carbamoyl phosphate synthetase produces and channels carbamoyl phosphate to the fused aspartate transcarbamoylase domain

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Random-priming in vitro recombination: an effective tool for directed evolution

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7 January 2021

Recombinant approaches for accessing biodiversity

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

A detailed study of the substrate specificity of a chimeric restriction enzyme.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

The state of antibody catalysis

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Thermostable chimeric PQQ glucose dehydrogenase.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Complexes of sequential metabolic enzymes

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Rational design of peptide antibiotics by targeted replacement of bacterial and fungal domains

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Insertion of new sequences into the catalytic domain of an enzyme

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Chimeras of human extracellular and intracellular superoxide dismutases. Analysis of structure and function of the individual domains

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Structural evidence for gene duplication in the evolution of the acid proteases

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Amino and carboxy-terminal regions in globular proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

The green fluorescent protein

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Structure of the multimodular endonuclease FokI bound to DNA

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

X-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Eye lens betaB2-crystallin: circular permutation does not influence the oligomerization state but enhances the conformational stability.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Circular permutation of betaB2-crystallin changes the hierarchy of domain assembly

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Synchronous digestion of SV40 DNA by exonuclease III.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Cytosine methylation targetted to pre-determined sequences

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: improvement of EDTA tolerance, thermal stability and substrate specificity

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Mapping catalytically important regions of an enzyme using two-codon insertion mutagenesis: a case study correlating beta-lactamase mutants with the three-dimensional structure

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Molecular evolution by staggered extension process (StEP) in vitro recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementatio

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Channeling of carbamoyl phosphate to the pyrimidine and arginine biosynthetic pathways in the deep sea hyperthermophilic archaeon Pyrococcus abyssi

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021

Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955002-0

7 January 2021