(Q28216587)

1

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J D Fox

3

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D E Anderson

4

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S Cherry

5

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T D Copeland

6

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D S Waugh

Protein Engineering Design and Selection

7

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language of work or name

English

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publication date

December 2001

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published in

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volume

14

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page(s)

993-1000

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issue

12

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cites work

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Recombinant protein expression in Escherichia coli

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Effect of substrate residues on the P2' preference of retroviral proteinases

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Preparation of an autolysis-resistant interleukin-1 beta converting enzyme mutant.

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Molecular genetic and biochemical evidence for the involvement of the heptapeptide cleavage sequence in determining the reaction profile at two tobacco etch virus cleavage sites in cell-free assays

21 January 2018

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Post-translational processing of the tobacco etch virus 49-kDa small nuclear inclusion polyprotein: Identification of an internal cleavage site and delimitation of VPg and proteinase domains

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Molecular genetic analysis of a plant virus polyprotein cleavage site: a model

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An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I

21 January 2018

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Autoproteolytic cleavage of recombinant 3C proteinase of hepatitis a virus

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Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold

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Site-directed mutagenesis by overlap extension using the polymerase chain reaction

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Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

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Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity.

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Gene fusion expression systems in Escherichia coli

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Autodigestion of lexA and phage lambda repressors

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Stabilization from autoproteolysis and kinetic characterization of the human T-cell leukemia virus type 1 proteinase

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Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins

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Engineering proteins to facilitate bioprocessing

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Cleavage profiles of tobacco etch virus (TEV)-derived substrates mediated by precursor and processed forms of the TEV NIa proteinase

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Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase

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Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form

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Auto-inactivation by cleavage within the dimer interface of Kaposi's sarcoma-associated herpesvirus protease

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Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants

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Design of high-throughput methods of protein production for structural biology

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Recombinant human cytomegalovirus protease with a C-terminal (His)6 extension: purification, autocatalytic release of the mature enzyme, and biochemical characterization.

21 January 2018

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A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli

21 January 2018

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Fusion proteins in biotechnology

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