(Q33712368)

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Hybrid enzymes

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scholarly article

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Europe PubMed Central

28 July 2017

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Europe PubMed Central

Hybrid enzymes (English)

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Europe PubMed Central

28 July 2017

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ORCID Public Data File 2021

author name string

Béguin P

1

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Europe PubMed Central

28 July 2017

publication date

1 August 1999

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Europe PubMed Central

28 July 2017

Current Opinion in Biotechnology

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Europe PubMed Central

28 July 2017

10

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Europe PubMed Central

28 July 2017

336-340

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Europe PubMed Central

28 July 2017

4

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Europe PubMed Central

28 July 2017

Hybrid enzymes: manipulating enzyme design.

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Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli

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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

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Hybrids of chicken cystatin with human kininogen domain 2 sequences exhibit novel inhibition of calpain, improved inhibition of actinidin and impaired inhibition of papain, cathepsin L and cathepsin B.

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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

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Immunogenicity of viral B- and T-cell epitopes expressed in recombinant bacterial proteins.

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Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains

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Expression of carboxymethylcellulase on the surface of Escherichia coli using Pseudomonas syringae ice nucleation protein

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Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae.

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Formation of bacterial membrane ice-nucleating lipoglycoprotein complexes.

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Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria

1 reference

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Structure of the cell wall anchor of surface proteins in Staphylococcus aureus

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Cell surface display of recombinant proteins on Staphylococcus carnosus

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Cell wall anchoring of the Streptococcus pyogenes M6 protein in various lactic acid bacteria.

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Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis

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OlpB, a new outer layer protein of Clostridium thermocellum, and binding of its S-layer-like domains to components of the cell envelope

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A conserved motif in S-layer proteins is involved in peptidoglycan binding in Thermus thermophilus

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Evidence that the N-terminal part of the S-layer protein from Bacillus stearothermophilus PV72/p2 recognizes a secondary cell wall polymer

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Production and cell surface anchoring of functional fusions between the SLH motifs of the Bacillus anthracis S-layer proteins and the Bacillus subtilis levansucrase

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Protein engineering and design for drug delivery

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CuZn superoxide dismutase (SOD-1):tetanus toxin fragment C hybrid protein for targeted delivery of SOD-1 to neuronal cells

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Substitution of the C-terminal domain of the Escherichia coli RNA polymerase alpha subunit by that from Bacillus subtilis makes the enzyme responsive to a Bacillus subtilis transcriptional activator

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A novel genetic system to detect protein-protein interactions

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Engineering of modular polyketide synthases to produce novel polyketides

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Harnessing the biosynthetic code: combinations, permutations, and mutations

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Construction of an artificial bifunctional enzyme, beta-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling.

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Characterization of a recombinant bifunctional enzyme, galactose dehydrogenase/bacterial luciferase, displaying an improved bioluminescence in a three-enzyme system

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Preparation and kinetic characterization of a fusion protein of yeast mitochondrial citrate synthase and malate dehydrogenase.

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The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation

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Properties conferred on Clostridium thermocellum endoglucanase CelC by grafting the duplicated segment of endoglucanase CelD.

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The cellulosome--a treasure-trove for biotechnology

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Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: prediction of specificity determinants of the dockerin domain

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Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein

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Homotropic Activation via the Subunit Interaction and Allosteric Symmetry Revealed on Analysis of Hybrid Enzymes ofl-Lactate Dehydrogenase

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Identifiers

10.1016/S0958-1669(99)80061-5

1 reference

Europe PubMed Central

28 July 2017

1 reference

Europe PubMed Central

28 July 2017

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