(Q34156498)

31 July 2017

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title

Watching proteins in the wild: fluorescence methods to study protein dynamics in living cells (English)

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31 July 2017

Chamberlain C

1

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PubMed ID

11208065

31 July 2017

Hahn KM

series ordinal

2

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31 July 2017

1 October 2000

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31 July 2017

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31 July 2017

1

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31 July 2017

755-762

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31 July 2017

10

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Fluorescently labelled molecules as probes of the structure and function of living cells.

31 July 2017

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Green fluorescent protein as a marker for gene expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Patterns of elevated free calcium and calmodulin activation in living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescence ratio imaging of cyclic AMP in single cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Improved green fluorescence

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Understanding, improving and using green fluorescent proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

FACS-optimized mutants of the green fluorescent protein (GFP)

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

The green fluorescent protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Applications of the green fluorescent protein in cell biology and biotechnology

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Green fluorescent protein: applications in cell biology

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Green fluorescent protein. The green revolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Ca2+ activation of hSlo K+ channel is suppressed by N-terminal GFP tag.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Cyanine dye labeling reagents for sulfhydryl groups

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescent actin analogs with a high affinity for profilin in vitro exhibit an enhanced gradient of assembly in living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Tat-mediated delivery of heterologous proteins into cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Five-parameter fluorescence imaging: wound healing of living Swiss 3T3 cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Dual-colour imaging with GFP variants

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Wavelength mutations and posttranslational autoxidation of green fluorescent protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Aequorea green fluorescent protein: simultaneous analysis of wild-type and blue-fluorescing mutant by flow cytometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescence Energy Transfer as a Spectroscopic Ruler

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Energy transfer: a spectroscopic ruler

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

A fluorescent protein biosensor of myosin II regulatory light chain phosphorylation reports a gradient of phosphorylated myosin II in migrating cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Single-molecule imaging of EGFR signalling on the surface of living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescence resonance energy transfer on single living cells. Application to binding of monovalent haptens to cell-bound immunoglobulin E

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Distribution of type I Fc epsilon-receptors on the surface of mast cells probed by fluorescence resonance energy transfer

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Role of metallothionein in nitric oxide signaling as revealed by a green fluorescent fusion protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Detection of programmed cell death using fluorescence energy transfer

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Using GFP in FRET-based applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Detection in living cells of Ca2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescent, sequence-selective peptide detection by synthetic small molecules

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Specific covalent labeling of recombinant protein molecules inside live cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Receptor-mediated targeting of fluorescent probes in living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Site-directed mutagenesis with an expanded genetic code.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Site-specific incorporation of novel backbone structures into proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

A general method for site-specific incorporation of unnatural amino acids into proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Site-specific incorporation of biophysical probes into proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Probing the mechanism of staphylococcal nuclease with unnatural amino acids: kinetic and structural studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Probing protein stability with unnatural amino acids

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Imaging protein kinase Calpha activation in cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Expressed protein ligation: a general method for protein engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Imaging of cAMP-dependent protein kinase activity in living neural cells using a novel fluorescent substrate.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescent proteins from nonbioluminescent Anthozoa species

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Imaging spatiotemporal dynamics of Rac activation in vivo with FLAIR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Fluorescent-protein biosensors: new tools for drug discovery

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

Chemical synthesis of peptides and proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

GFP variants for multispectral imaging of living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Quantitative imaging of the green fluorescent protein (GFP)

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Electroporation-mediated uptake of proteins into mammalian cells

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Osmotic lysis of pinosomes

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Two-color green fluorescent protein time-lapse imaging.

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Development and applications of enhanced green fluorescent protein mutants.

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Resonance energy transfer microscopy.

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Cyclic AMP-dependent protein kinase binding to A-kinase anchoring proteins in living cells by fluorescence resonance energy transfer of green fluorescent protein fusion proteins

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

A genetically encoded, fluorescent indicator for cyclic AMP in living cells

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Green fluorescent protein labeling of cytoskeletal structures--novel targeting approach based on leucine zippers

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

15 Biosynthetic method for introducing unnatural amino acids site-specifically into proteins

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Localized Rac activation dynamics visualized in living cells

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

Site-Specific Protein Modification Using a Ketone Handle

1 reference

https://api.crossref.org/works/10.1034%2FJ.1600-0854.2000.011002.X

7 January 2021

10.1034/J.1600-0854.2000.011002.X

Identifiers

DOI

1 reference

31 July 2017

1 reference

PubMed ID

11208065