(Q34614856)

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Genome engineering

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scholarly article

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Europe PubMed Central

3 August 2017

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Europe PubMed Central

Genome engineering (English)

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Europe PubMed Central

3 August 2017

George M. Church

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George M Church

2

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author name string

Peter A Carr

1

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Europe PubMed Central

3 August 2017

publication date

1 December 2009

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Europe PubMed Central

3 August 2017

Nature Biotechnology

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Europe PubMed Central

3 August 2017

27

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Europe PubMed Central

3 August 2017

1151-1162

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Europe PubMed Central

3 August 2017

12

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Europe PubMed Central

3 August 2017

https://scigraph.springernature.com/pub.10.1038/nbt.1590

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Towards synthesis of a minimal cell

1 reference

https://api.crossref.org/works/10.1038%2FNBT.1590

21 January 2018

Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction

1 reference

https://api.crossref.org/works/10.1038%2FNBT.1590

21 January 2018

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

1 reference

https://api.crossref.org/works/10.1038%2FNBT.1590

21 January 2018

Writing DNA with GenoCAD

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Synthetic biology projects in vitro

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https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Chromosome transfer between Escherichia coli HFR strains and Proteus mirabilis

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Programming cells by multiplex genome engineering and accelerated evolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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A method for linking yeast artificial chromosomes.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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A scalable, GFP-based pipeline for membrane protein overexpression screening and purification

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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A network of orthogonal ribosome x mRNA pairs.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Characterization of the reconstructed 1918 Spanish influenza pandemic virus

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Global transposon mutagenesis and a minimal Mycoplasma genome

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Combinatorial engineering of microbes for optimizing cellular phenotype

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https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Manipulation of human minichromosomes to carry greater than megabase-sized chromosome inserts

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https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Transposon-mediated genome manipulation in vertebrates

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https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Superpositioning of deletions promotes growth of Escherichia coli with a reduced genome

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https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Generation of cDNA expression libraries enriched for in-frame sequences

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https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Engineering ligand-responsive gene-control elements: lessons learned from natural riboswitches

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Sequencing genomes from single cells by polymerase cloning

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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A totally synthetic plasmid for general cloning, gene expression and mutagenesis in Escherichia coli.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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In vitro selection of functional nucleic acids

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Synthetic biology--putting engineering into biology

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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The second wave of synthetic biology: from modules to systems

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Genetically modified organisms for the environment: stories of success and failure and what we have learned from them.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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A systems approach to measuring the binding energy landscapes of transcription factors

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Engineering a reduced Escherichia coli genome

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Solid-Phase Oligodeoxynucleotide Synthesis: A Two-Step Cycle Using Peroxy Anion Deprotection

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Gene synthesis by circular assembly amplification.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Diversity-based, model-guided construction of synthetic gene networks with predicted functions

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Programmable cells: interfacing natural and engineered gene networks

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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In vivo and in vitro protein solubility assays using split GFP.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 1. General introduction

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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A novel solid phase technology for high-throughput gene synthesis.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Measuring the activity of BioBrick promoters using an in vivo reference standard

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Refinement and standardization of synthetic biological parts and devices

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Synthetic gene networks that count

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Cell-free cloning using phi29 DNA polymerase

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Microbial sensors for small molecules: development of a mevalonate biosensor

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Advances in synthetic biology: on the path from prototypes to applications

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Advanced sequencing technologies: methods and goals

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Chemical gene synthesis: strategies, softwares, error corrections, and applications

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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A synthetic multicellular system for programmed pattern formation

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Parallel gene synthesis in a microfluidic device

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Protein synthesis by pure translation systems.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A rapid method for the construction of synthetic genes using the polymerase chain reaction

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Correcting errors in synthetic DNA through consensus shuffling

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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De novo DNA synthesis using single molecule PCR.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Engineering life: building a fab for biology

2 references

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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https://api.crossref.org/works/10.1038%2FNBT.1590

7 January 2021

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Foundations for engineering biology

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Emergent properties of reduced-genome Escherichia coli.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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TABASCO: A single molecule, base-pair resolved gene expression simulator

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Essential genes of a minimal bacterium

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Protein-mediated error correction for de novo DNA synthesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Protein fabrication automation

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Next-generation synthetic gene networks

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Studies on the chemical synthesis and enzymatic degradation of desoxyribo-oligonucleotides

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Two-step total gene synthesis method

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Systematic assembly of a full-length infectious clone of human coronavirus NL63

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Creating bacterial strains from genomes that have been cloned and engineered in yeast

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Refactoring bacteriophage T7

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Next-generation DNA sequencing

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Recursive PCR: a novel technique for total gene synthesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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The pace and proliferation of biological technologies.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Genome transplantation in bacteria: changing one species to another

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Total synthesis of a tyrosine suppressor tRNA gene. XVIII. Biological activity and transcription, in vitro, of the cloned gene

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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A B cell-based sensor for rapid identification of pathogens

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Accurate multiplex gene synthesis from programmable DNA microchips

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Environmentally controlled invasion of cancer cells by engineered bacteria

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

based on heuristic

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Implications of rewiring bacterial quorum sensing

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Gene synthesis demystified

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Accurate multiplex polony sequencing of an evolved bacterial genome

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Reassembly of shattered chromosomes in Deinococcus radiodurans

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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De novo computational design of retro-aldol enzymes

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Toward scalable parts families for predictable design of biological circuits

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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PCR-based gene synthesis to produce recombinant proteins for crystallization.

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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A bacterial protein enhances the release and efficacy of liposomal cancer drugs

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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New microbial fuels: a biotech perspective

1 reference

https://pubmed.ncbi.nlm.nih.gov/20010598

12 December 2020

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Nucleotides part XXXII. Synthesis of a dithymidine dinucleotide containing a 3′: 5′-internucleotidic linkage

1 reference

https://api.crossref.org/works/10.1038%2FNBT.1590

7 January 2021

based on heuristic

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Engineered human cells: say no to sepsis

1 reference

https://api.crossref.org/works/10.1038%2FNBT.1590

7 January 2021

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The chemical synthesis of oligo- and poly-nucleotides: a personal commentary

1 reference

https://api.crossref.org/works/10.1038%2FNBT.1590

7 January 2021

based on heuristic

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Identifiers

10.1038/NBT.1590

1 reference

Europe PubMed Central

3 August 2017

Dimensions Publication ID

0 references

1 reference

Europe PubMed Central

3 August 2017

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