(Q34683632)

3 August 2017

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title

Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream (English)

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3 August 2017

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David G Ginzinger

1

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3 August 2017

1 June 2002

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3 August 2017

Experimental Hematology

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3 August 2017

30

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3 August 2017

503-512

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3 August 2017

6

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Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

3 August 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

A novel method for real time quantitative RT-PCR.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Real time quantitative PCR

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https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Molecular beacons: probes that fluoresce upon hybridization

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Comparison between Taq DNA polymerase and its Stoffel fragment for quantitative real-time PCR with hybridization probes

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

A closed tube format for amplification and detection of DNA based on energy transfer

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Rapid detection of the 22q11.2 deletion with quantitative real-time PCR.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Detection of 1p and 19q loss in oligodendroglioma by quantitative microsatellite analysis, a real-time quantitative polymerase chain reaction assay

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative real-time PCR assay for determining transgene copy number in transformed plants

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Use of single nucleotide polymorphisms (SNP) and real-time polymerase chain reaction for bone marrow engraftment analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Evidence for an interferon-inducible gene, Ifi202, in the susceptibility to systemic lupus

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Identification of myelodysplastic syndrome-specific genes by DNA microarray analysis with purified hematopoietic stem cell fraction

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Induction of hypervascularity without leakage or inflammation in transgenic mice overexpressing hypoxia-inducible factor-1alpha

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Detection of gene amplification in intraductal and infiltrating breast cancer by laser-assisted microdissection and quantitative real-time PCR.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5' nuclease quantitative reverse transcription-polymerase chain reaction

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Global cDNA amplification combined with real-time RT-PCR: accurate quantification of multiple human potassium channel genes at the single cell level

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Higher LPS-stimulated TNF-alpha mRNA levels in peripheral blood mononuclear cells from non-Hodgkin's lymphoma patients

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

A validated real-time quantitative PCR approach shows a correlation between tumor burden and successful ex vivo purging in follicular lymphoma patients.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

The involvement of human-nuc gene in polyploidization of K562 cell line.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Overexpression of GATA-2 inhibits erythroid and promotes megakaryocyte differentiation.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitation of minimal residual disease in multiple myeloma using an allele-specific real-time PCR assay

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative monitoring of the PRAME gene for the detection of minimal residual disease in leukaemia

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Detection of minimal residual disease in patients with AML1/ETO-associated acute myeloid leukemia using a novel quantitative reverse transcription polymerase chain reaction assay

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

The AML1/MTG8 Fusion Transcript in t(8;21) Positive AML and its Implication for the Detection of Minimal Residual Disease

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

The amount of BCR-ABL fusion transcripts detected by the real-time quantitative polymerase chain reaction method in patients with Philadelphia chromosome positive chronic myeloid leukemia correlates with the disease stage

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Expression of interferon-alpha (IFN-alpha) receptor 2c at diagnosis is associated with cytogenetic response in IFN-alpha-treated chronic myeloid leukemia

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Application of germline IGH probes in real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Novel 5' exonuclease-based real-time PCR assay for the detection of t(14;18)(q32;q21) in patients with follicular lymphoma

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Real-time 5'-->3' exonuclease-based PCR assay for detection of the t(11;14)(q13;q32)

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Differential mobilization of CD34+ cells and lymphoma cells in non-Hodgkin's lymphoma patients mobilized with different growth factors.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Frequency of the Bcl-2/IgH rearrangement in normal individuals: implications for the monitoring of disease in patients with follicular lymphoma

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Use of a fluorescent-PCR reaction to detect genomic sequence copy number and transcriptional abundance

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Rapid quantitation of proinflammatory and chemoattractant cytokine expression in small tissue samples and monocyte-derived dendritic cells: validation of a new real-time RT-PCR technology

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Expression and functional analysis of chemokine receptors in human peripheral blood leukocyte populations

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative molecular analysis of laser-microdissected paraffin-embedded human tissues

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative amplification of genomic DNA from histological tissue sections after staining with nuclear dyes and laser capture microdissection

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Real-time quantitative RT-PCR after laser-assisted cell picking

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Detection of gene amplification in archival breast cancer specimens by laser-assisted microdissection and quantitative real-time polymerase chain reaction

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative detection of t(14;18)-positive cells by real-time quantitative PCR using fluorogenic probes

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Detection of minimal residual disease

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

A quantitative, internally controlled real-time PCR Assay for the detection of parvovirus B19 DNA

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Real-time quantitative PCR for retrovirus-like particle quantification in CHO cell culture

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Use of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus levels in mAb cell-culture and downstream processing

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Development of a model system to compare cell separation methods for the isolation of fetal cells from maternal blood

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis.

1 reference

https://api.crossref.org/works/10.1016%2FS0301-472X%2802%2900806-8

7 January 2021

10.1016/S0301-472X(02)00806-8

Identifiers

DOI

1 reference

3 August 2017

1 reference

PubMed ID

12063017