(Q35182675)

7 August 2017

Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations (English)

1 reference

7 August 2017

0 references

5

Ulrich Schwaneberg

1 reference

7 August 2017

3

Anna Joëlle Ruff

1 reference

7 August 2017

Jing Zhao

1

1 reference

PubMed ID

24904008

7 August 2017

Tsvetan Kardashliev

series ordinal

2

1 reference

PubMed ID

24904008

7 August 2017

Marco Bocola

series ordinal

4

1 reference

7 August 2017

14 July 2014

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Biotechnology and Bioengineering

7 August 2017

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7 August 2017

111

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7 August 2017

2380-2389

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7 August 2017

12

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Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics

7 August 2017

1 reference

Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

21 January 2018

1 reference

Protein stability promotes evolvability

21 January 2018

1 reference

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.

21 January 2018

1 reference

Generating mutant libraries using error-prone PCR.

21 January 2018

1 reference

WebLogo: A Sequence Logo Generator

21 January 2018

1 reference

OmniChange: the sequence independent method for simultaneous site-saturation of five codons

21 January 2018

1 reference

Protein engineering by random mutagenesis and structure-guided consensus of Geobacillus stearothermophilus Lipase T6 for enhanced stability in methanol

21 January 2018

1 reference

Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins

21 January 2018

1 reference

A novel random mutagenesis approach using human mutagenic DNA polymerases to generate enzyme variant libraries.

21 January 2018

1 reference

PFunkel: efficient, expansive, user-defined mutagenesis.

21 January 2018

1 reference

The genetic code constrains yet facilitates Darwinian evolution

21 January 2018

1 reference

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

21 January 2018

1 reference

A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library

21 January 2018

1 reference

Design of an activity and stability improved carbonyl reductase from Candida parapsilosis

21 January 2018

1 reference

A high-throughput screening method to reengineer DNA polymerases for random mutagenesis

21 January 2018

1 reference

Understanding a mechanism of organic cosolvent inactivation in heme monooxygenase P450 BM-3

21 January 2018

1 reference

High-throughput screening for terpene-synthase-cyclization activity and directed evolution of a terpene synthase

21 January 2018

1 reference

Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution

21 January 2018

1 reference

SeSaM-Tv-II generates a protein sequence space that is unobtainable by epPCR.

21 January 2018

1 reference

Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

21 January 2018

1 reference

Critical evaluation of random mutagenesis by error-prone polymerase chain reaction protocols, Escherichia coli mutator strain, and hydroxylamine treatment.

21 January 2018

1 reference

To get what we aim for--progress in diversity generation methods

21 January 2018

1 reference

P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation

21 January 2018

1 reference

Multi-site saturation by OmniChange yields a pH- and thermally improved phytase

21 January 2018

1 reference

Directed evolution of a highly active Yersinia mollaretii phytase.

21 January 2018

1 reference

Directed evolution of oxygenases: screening systems, success stories and challenges

21 January 2018

1 reference

Polishing the craft of genetic diversity creation in directed evolution

21 January 2018

1 reference

Directed evolution strategies for enantiocomplementary haloalkane dehalogenases: from chemical waste to enantiopure building blocks

21 January 2018

1 reference

The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme

21 January 2018

1 reference

Reducing mutational bias in random protein libraries

21 January 2018

1 reference

MAP(2.0)3D: a sequence/structure based server for protein engineering

21 January 2018

1 reference

A computer program for the estimation of protein and nucleic acid sequence diversity in random point mutagenesis libraries

21 January 2018

1 reference

Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR.

21 January 2018

1 reference

A statistical analysis of random mutagenesis methods used for directed protein evolution.

21 January 2018

1 reference

Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution

21 January 2018

1 reference

An efficient one-step site-directed and site-saturation mutagenesis protocol

21 January 2018

1 reference

Directed Evolution of an EnantioselectiveBacillus subtilisLipase

21 January 2018

1 reference

Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis

21 January 2018

1 reference

Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

21 January 2018

1 reference

dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method

21 January 2018

1 reference

Challenges of the genetic code for exploring sequence space in directed protein evolution

21 January 2018

1 reference

Widening the pH activity profile of a fungal laccase by directed evolution

21 January 2018

1 reference

21 January 2018

Identifiers

DOI

10.1002/BIT.25302

1 reference

7 August 2017

1 reference

PubMed ID

24904008