(Q35222433)

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SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution

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scholarly article

1 reference

Europe PubMed Central

7 August 2017

SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution (English)

1 reference

Europe PubMed Central

7 August 2017

protein evolution

0 references

4

1 reference

Europe PubMed Central

7 August 2017

2

object named as

Neil Swainston

1 reference

Europe PubMed Central

7 August 2017

author name string

Andrew Currin

1

1 reference

Europe PubMed Central

7 August 2017

Philip J Day

3

1 reference

Europe PubMed Central

7 August 2017

publication date

9 August 2014

1 reference

Europe PubMed Central

7 August 2017

Protein Engineering Design and Selection

1 reference

Europe PubMed Central

7 August 2017

27

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Europe PubMed Central

7 August 2017

273-280

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Europe PubMed Central

7 August 2017

9

1 reference

Europe PubMed Central

7 August 2017

GeneGenie: optimized oligomer design for directed evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Recent advances in rational approaches for enzyme engineering

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Engineering an enantioselective amine oxidase for the synthesis of pharmaceutical building blocks and alkaloid natural products

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Towards practical, high-capacity, low-maintenance information storage in synthesized DNA

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Navigating the protein fitness landscape with Gaussian processes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

DNA synthesis, assembly and applications in synthetic biology

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Scientific discovery as a combinatorial optimisation problem: how best to navigate the landscape of possible experiments?

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Error correction in gene synthesis technology

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Error correction of microchip synthesized genes using Surveyor nuclease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

The imminent role of protein engineering in synthetic biology

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

SeSaM-Tv-II generates a protein sequence space that is unobtainable by epPCR.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

DNA assembly for synthetic biology: from parts to pathways and beyond.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Efficient assembly of very short oligonucleotides using T4 DNA Ligase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Creation of a bacterial cell controlled by a chemically synthesized genome

1 reference

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27 July 2018

Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Genome engineering

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Directed evolution drives the next generation of biocatalysts

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Enzymatic assembly of DNA molecules up to several hundred kilobases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

The structure of monoamine oxidase from Aspergillus niger provides a molecular context for improvements in activity obtained by directed evolution

1 reference

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27 July 2018

Chemical gene synthesis: strategies, softwares, error corrections, and applications

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Enzyme optimization: moving from blind evolution to statistical exploration of sequence-function space

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Expanding the substrate scope of enzymes: combining mutations obtained by CASTing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Many paths to many clones: a comparative look at high-throughput cloning methods

1 reference

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27 July 2018

A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Directed evolution of enzymes: new biocatalysts for asymmetric synthesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Deracemization of alpha-methylbenzylamine using an enzyme obtained by in vitro evolution

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo

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27 July 2018

Rapid evolution of a protein in vitro by DNA shuffling

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27 July 2018

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

27 July 2018

Oligonucleotide assembly in yeast to produce synthetic DNA fragments

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4140418

26 September 2018

Classifying DNA assembly protocols for devising cellular architectures

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26 September 2018

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26 September 2018

Advancing high-throughput gene synthesis technology

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26 September 2018

In silico modelling of directed evolution: Implications for experimental design and stepwise evolution.

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26 September 2018

A modified method for PCR-directed gene synthesis from large number of overlapping oligodeoxyribonucleotides.

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26 September 2018

Gene synthesis by circular assembly amplification.

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26 September 2018

Efficient site-directed saturation mutagenesis using degenerate oligonucleotides.

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26 September 2018

An improvement of the site-directed mutagenesis method by combination of megaprimer, one-side PCR and DpnI treatment.

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26 September 2018

Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences

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26 September 2018

DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis

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26 September 2018

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability

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26 September 2018

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space

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26 September 2018

The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase

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26 September 2018

A general model of error-prone PCR

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Patch oligodeoxynucleotide synthesis (POS): a novel method for synthesis of long DNA sequences and full-length genes

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Industrial scale gene synthesis

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based on heuristic

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Identifiers

10.1093/PROTEIN/GZU029

1 reference

Europe PubMed Central

7 August 2017

1 reference

Europe PubMed Central

7 August 2017

1 reference

Europe PubMed Central

7 August 2017

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