(Q37947529)

25 August 2017

The mechanism of the second step of pre-mRNA splicing (English)

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25 August 2017

David S Horowitz

1

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25 August 2017

19 October 2011

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Wiley interdisciplinary reviews. RNA

25 August 2017

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25 August 2017

3

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25 August 2017

331-350

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25 August 2017

3

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Evolution of yeast noncoding RNAs reveals an alternative mechanism for widespread intron loss

25 August 2017

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Genomic-scale quantitative analysis of yeast pre-mRNA splicing: implications for splice-site recognition

21 January 2018

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Comprehensive splice-site analysis using comparative genomics

21 January 2018

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Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae

21 January 2018

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Introns and splicing elements of five diverse fungi

21 January 2018

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Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron

21 January 2018

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Scanning from an independently specified branch point defines the 3' splice site of mammalian introns

21 January 2018

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Repositioning of the reaction intermediate within the catalytic center of the spliceosome.

21 January 2018

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Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S. cerevisiae.

21 January 2018

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DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps

21 January 2018

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Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeast

21 January 2018

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Evidence for a linear search in bimolecular 3' splice site AG selection

21 January 2018

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An upstream AG determines whether a downstream AG is selected during catalytic step II of splicing

21 January 2018

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The role of branchpoint-3' splice site spacing and interaction between intron terminal nucleotides in 3' splice site selection in Saccharomyces cerevisiae

21 January 2018

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A U-rich tract enhances usage of an alternative 3′ splice site in yeast

21 January 2018

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Evidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammals

21 January 2018

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Scanning and competition between AGs are involved in 3' splice site selection in mammalian introns

21 January 2018

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A novel role for a U5 snRNP protein in 3' splice site selection.

21 January 2018

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Exon sequences at the splice junctions affect splicing fidelity and alternative splicing

21 January 2018

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Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing

21 January 2018

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The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing

21 January 2018

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The spliceosome: design principles of a dynamic RNP machine

21 January 2018

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Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45

21 January 2018

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Mechanical devices of the spliceosome: motors, clocks, springs, and things

21 January 2018

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Mutations in U6 snRNA that alter splice site specificity: implications for the active site

21 January 2018

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Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps

21 January 2018

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A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing

21 January 2018

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A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome

21 January 2018

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Multiple functions for the invariant AGC triad of U6 snRNA.

21 January 2018

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A structural analysis of the group II intron active site and implications for the spliceosome

21 January 2018

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Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome.

21 January 2018

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Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits

21 January 2018

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Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing

21 January 2018

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Splicing function of mammalian U6 small nuclear RNA: conserved positions in central domain and helix I are essential during the first and second step of pre-mRNA splicing

21 January 2018

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Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicingin vitro

21 January 2018

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Randomization-selection analysis of snRNAs in vivo: evidence for a tertiary interaction in the spliceosome

21 January 2018

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A tertiary interaction detected in a human U2-U6 snRNA complex assembled in vitro resembles a genetically proven interaction in yeast

21 January 2018

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Mutational analysis of the yeast U2 snRNA suggests a structural similarity to the catalytic core of group I introns

21 January 2018

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Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing

21 January 2018

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Identification of a U2/U6 helix la mutant that influences 3' splice site selection during nuclear pre-mRNA splicing

21 January 2018

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Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.

21 January 2018

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Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center

21 January 2018

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Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants.

21 January 2018

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Exon ligation is proofread by the DExD/H-box ATPase Prp22p

21 January 2018

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U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing

21 January 2018

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Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing

21 January 2018

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Architecture of the U5 small nuclear RNA

21 January 2018

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Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.

21 January 2018

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Mutations in U5 snRNA loop 1 influence the splicing of different genes in vivo

21 January 2018

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Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing

21 January 2018

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Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components

21 January 2018

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PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome

21 January 2018

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Stages in the second reaction of pre-mRNA splicing: the final step is ATP independent.

21 January 2018

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Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing

21 January 2018

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Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome

21 January 2018

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Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.

21 January 2018

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A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation

21 January 2018

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A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing

21 January 2018

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RNA helicases at work: binding and rearranging

21 January 2018

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PRP16, a DEAH-box RNA helicase, is recruited to the spliceosome primarily via its nonconserved N-terminal domain

21 January 2018

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Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing

21 January 2018

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An essential splicing factor, SLU7, mediates 3' splice site choice in yeast

21 January 2018

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Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18.

21 January 2018

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How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing

21 January 2018

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Crystal structure of the functional domain of the splicing factor Prp18

21 January 2018

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A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae

21 January 2018

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Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA.

21 January 2018

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Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes

21 January 2018

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RNA helicase dynamics in pre-mRNA splicing

21 January 2018

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Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22.

21 January 2018

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Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.

21 January 2018

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Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly

21 January 2018

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Prp43: An RNA helicase-like factor involved in spliceosome disassembly

21 January 2018

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Mutations in PRP43 that uncouple RNA-dependent NTPase activity and pre-mRNA splicing function

21 January 2018

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Prp43p contains a processive helicase structural architecture with a specific regulatory domain

21 January 2018

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Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome

21 January 2018

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Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing

21 January 2018

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Structural basis for the function of DEAH helicases

21 January 2018

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Prp8 protein: at the heart of the spliceosome

21 January 2018

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Structural studies of the spliceosome: blind men and an elephant

21 January 2018

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The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein

21 January 2018

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Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing

21 January 2018

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Motifs IV and V in the DEAH box splicing factor Prp22 are important for RNA unwinding, and helicase-defective Prp22 mutants are suppressed by Prp8.

21 January 2018

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Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing

21 January 2018

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The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome

21 January 2018

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Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing

21 January 2018

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Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast

21 January 2018

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Genome-wide analysis of pre-mRNA splicing: intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe

21 January 2018

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Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction

21 January 2018

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A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing

21 January 2018

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The splice is right: guarantors of fidelity in pre-mRNA splicing

21 January 2018

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The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing

21 January 2018

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Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast

21 January 2018

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Bimolecular exon ligation by the human spliceosome bypasses early 3' splice site AG recognition and requires NTP hydrolysis

21 January 2018

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Functional and physical interaction between the yeast splicing factors Slu7 and Prp18.

21 January 2018

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The RNA splicing factor hSlu7 is required for correct 3' splice-site choice

21 January 2018

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Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome

21 January 2018

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The question remains: is the spliceosome a ribozyme?

21 January 2018

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Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing

21 January 2018

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A general two-metal-ion mechanism for catalytic RNA

21 January 2018

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Is the spliceosome a ribonucleoprotein enzyme?

21 January 2018

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Metal ion catalysis during the exon-ligation step of nuclear pre-mRNA splicing: extending the parallels between the spliceosome and group II introns

21 January 2018

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A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release

21 January 2018

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A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome

21 January 2018

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The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase

21 January 2018

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Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis

21 January 2018

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Isolation of an active step I spliceosome and composition of its RNP core.

21 January 2018

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Pre-mRNA splicing: awash in a sea of proteins

21 January 2018

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The mammalian homologue of Prp16p is overexpressed in a cell line tolerant to Leflunomide, a new immunoregulatory drug effective against rheumatoid arthritis

21 January 2018

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Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing

21 January 2018

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A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor

21 January 2018

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Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22

21 January 2018

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An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2.

21 January 2018

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The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes

21 January 2018

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A cyclophilin functions in pre-mRNA splicing

21 January 2018

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RS domain-splicing signal interactions in splicing of U12-type and U2-type introns.

21 January 2018

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21 January 2018

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Ser/Thr-specific protein phosphatases are required for both catalytic steps of pre-mRNA splicing

21 January 2018

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Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis

21 January 2018

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Spliceosomal immunophilins

21 January 2018

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Protein components specifically associated with prespliceosome and spliceosome complexes

21 January 2018

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Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis

21 January 2018

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U2AF-homology motif interactions are required for alternative splicing regulation by SPF45

21 January 2018

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21 January 2018

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21 January 2018

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Kinetic amplification of enzyme discrimination

21 January 2018

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Features of spliceosome evolution and function inferred from an analysis of the information at human splice sites.

21 January 2018

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The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing

21 January 2018

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Spliceosome discards intermediates via the DEAH box ATPase Prp43p

21 January 2018

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Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function

21 January 2018

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Assembly, disassembly and recycling: the dynamics of exon junction complexes

21 January 2018

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The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay

21 January 2018

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Introns play an essential role in splicing-dependent formation of the exon junction complex.

21 January 2018

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U2–U6 RNA folding reveals a group II intron-like domain and a four-helix junction

21 January 2018

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Intron Removal Requires Proofreading of U2AF/3' Splice Site Recognition by DEK

21 January 2018

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Metal ion catalysis during splicing of premessenger RNA

21 January 2018

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Evidence for an essential non-Watson–Crick interaction between the first and last nucleotides of a nuclear pre-mRNA intron

21 January 2018

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Cleavage of 5′ splice site and lariat formation are independent of 3′ splice site in yeast mRNA splicing

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/WRNA.112

1 reference

25 August 2017

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PubMed ID

22012849