(Q39066272)

8 September 2017

title

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology (English)

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8 September 2017

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Randall A Hughes

1

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8 September 2017

Andrew D Ellington

series ordinal

2

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8 September 2017

publication date

3 January 2017

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Cold Spring Harbor Perspectives in Biology

8 September 2017

published in

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8 September 2017

volume

9

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8 September 2017

issue

1

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Total synthesis of a functional designer eukaryotic chromosome.

8 September 2017

cites work

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Gene synthesis by a LCR-based approach: high-level production of leptin-L54 using synthetic gene in Escherichia coli

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Correcting errors in synthetic DNA through consensus shuffling

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Antisense transcription as a tool to tune gene expression

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Genetic switchboard for synthetic biology applications

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Protein-mediated error correction for de novo DNA synthesis

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A brief review of DNA and RNA chemical synthesis

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High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer

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Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis

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Gene synthesis demystified

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Synthetic generation of influenza vaccine viruses for rapid response to pandemics

21 January 2018

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Genome editing. The new frontier of genome engineering with CRISPR-Cas9

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A one pot, one step, precision cloning method with high throughput capability

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Light-directed, spatially addressable parallel chemical synthesis

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Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

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A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids

21 January 2018

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Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

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Enzymatic assembly of overlapping DNA fragments

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Gene and genome construction in yeast

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Oligonucleotide assembly in yeast to produce synthetic DNA fragments

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Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome

21 January 2018

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One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

21 January 2018

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Enzymatic assembly of DNA molecules up to several hundred kilobases

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Chemical synthesis of the mouse mitochondrial genome.

21 January 2018

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Rapid, modular and reliable construction of complex mammalian gene circuits

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Gene synthesis: methods and applications

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Design and synthesis of a minimal bacterial genome

21 January 2018

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Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin

21 January 2018

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Polymerase chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme c of horseradish peroxidase

21 January 2018

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Studies on polynucleotides. 103. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast

21 January 2018

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'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules

21 January 2018

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Multiplex pairwise assembly of array-derived DNA oligonucleotides

21 January 2018

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Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster.

21 January 2018

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Large-scale de novo DNA synthesis: technologies and applications

21 January 2018

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Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

21 January 2018

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BglBrick vectors and datasheets: A synthetic biology platform for gene expression.

21 January 2018

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Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

21 January 2018

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Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

21 January 2018

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DNA synthesis, assembly and applications in synthetic biology

21 January 2018

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High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

21 January 2018

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Genetic circuit design automation

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Massively parallel functional dissection of mammalian enhancers in vivo

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Light-generated oligonucleotide arrays for rapid DNA sequence analysis

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Circular polymerase extension cloning of complex gene libraries and pathways

21 January 2018

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Parallel on-chip gene synthesis and application to optimization of protein expression.

21 January 2018

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MerMade: an oligodeoxyribonucleotide synthesizer for high throughput oligonucleotide production in dual 96-well plates

21 January 2018

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Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Synthesis of DNA/RNA and their analogs via phosphoramidite and H-phosphonate chemistries

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Synthetic mixed-signal computation in living cells

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Error correction of microchip synthesized genes using Surveyor nuclease

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Automated design of synthetic ribosome binding sites to control protein expression

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

The rise of a digital immune system

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Engineering BioBrick vectors from BioBrick parts

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Advances in synthetic dynamic circuits design: using novel synthetic parts to engineer new generations of gene oscillations

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

21 January 2018

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https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Accurate multiplex gene synthesis from programmable DNA microchips

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

PaperClip: rapid multi-part DNA assembly from existing libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Error removal in microchip-synthesized DNA using immobilized MutS.

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Programming cells by multiplex genome engineering and accelerated evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Chemical gene synthesis: strategies, softwares, error corrections, and applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Non-polymerase-cycling-assembly-based chemical gene synthesis: strategies, methods, and progress

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Two-step total gene synthesis method

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1101%2FCSHPERSPECT.A023812

Stepwise synthesis of deoxyribo-oligonucleotides

21 January 2018

1 reference

12 December 2020

In situ Synthesis of DNA Microarray on Functionalized Cyclic Olefin Copolymer Substrate

1 reference

12 December 2020

Total synthesis of a tyrosine suppressor transfer RNA gene. XVI. Enzymatic joinings to form the total 207-base pair-long DNA

1 reference

12 December 2020

A rapid method for the construction of synthetic genes using the polymerase chain reaction

1 reference

12 December 2020

Rapid and reliable DNA assembly via ligase cycling reaction

1 reference

12 December 2020

Progress in the total synthesis of the gene for ala-tRNA

1 reference

12 December 2020

Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 7. Enzymatic joining of the chemically synthesized segments to form a DNA duplex corresponding to the nucleotide sequence 1-26

1 reference

12 December 2020

10.1101/CSHPERSPECT.A023812

Identifiers

DOI

1 reference

8 September 2017

0 references

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