(Q39723342)

14 September 2017

An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes (English)

1 reference

14 September 2017

Kirsch RD

1

1 reference

PMCID

147442

14 September 2017

Joly E

series ordinal

2

1 reference

PMCID

147442

14 September 2017

language of work or name

English

0 references

publication date

1 April 1998

1 reference

14 September 2017

Nucleic Acids Research

1 reference

14 September 2017

26

1 reference

14 September 2017

1848-1850

1 reference

14 September 2017

7

1 reference

Random-priming in vitro recombination: an effective tool for directed evolution

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

Modified base compositions at degenerate positions of a mutagenic oligonucleotide enhance randomness in site-saturation mutagenesis

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

"Megaprimer" method of PCR-based mutagenesis: the concentration of megaprimer is a critical factor.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

An efficient PCR mutagenesis strategy without gel purification [correction of "purificiation"] step that is amenable to automation

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

The "megaprimer" method of site-directed mutagenesis

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

The rat MHC haplotype RT1c expresses two classical class I molecules.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

Isolation of a functional cDNA encoding the RT1.Au MHC class I heavy chain by a novel PCR-based method.

25 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=147442

Megaprimer method for in vitro mutagenesis using parallel templates

20 August 2018

1 reference

12 December 2020

Gene splicing by overlap extension

1 reference

12 December 2020

PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes

1 reference

12 December 2020

Two versatile eukaryotic vectors permitting epitope tagging, radiolabelling and nuclear localisation of expressed proteins

1 reference

12 December 2020

Genes in two MHC class I regions control recognition of a single rat NK cell allodeterminant

1 reference

12 December 2020

PCR mutagenesis: treatment of the megaprimer with mung bean nuclease improves yield

1 reference

12 December 2020

"Megaprimer" method of PCR: increased template concentration improves yield

1 reference

12 December 2020

Modification of the megaprimer method of PCR mutagenesis: improved amplification of the final product

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/26.7.1848

1 reference

14 September 2017

1 reference

14 September 2017

1 reference

PMCID

147442