(Q43690465)

25 November 2017

Escherichia coli SecA helicase activity is not required in vivo for efficient protein translocation or autogenous regulation (English)

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25 November 2017

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M O Schmidt

1

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25 November 2017

R M Brosh

series ordinal

2

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25 November 2017

D B Oliver

series ordinal

3

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25 November 2017

language of work or name

English

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publication date

27 July 2001

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Journal of Biological Chemistry

25 November 2017

10

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based on heuristic

inferred from page(s)

published in

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25 November 2017

276

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25 November 2017

37076-37085

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25 November 2017

40

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Escherichia coli translocase: the unravelling of a molecular machine

25 November 2017

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecYEG assembles into a tetramer to form the active protein translocation channel

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Roles of SecG in ATP- and SecA-dependent protein translocation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The catalytic cycle of the escherichia coli SecA ATPase comprises two distinct preprotein translocation events

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A significant fraction of functional SecA is permanently embedded in the membrane. SecA cycling on and off the membrane is not essential during protein translocation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Dual regulation of Escherichia coli secA translation by distinct upstream elements

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Identification of elements on GeneX-secA RNA of Escherichia coli required for SecA binding and secA auto-regulation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Secretion monitor, SecM, undergoes self-translation arrest in the cytosol

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Autogenous translation regulation by Escherichia coli ATPase SecA may be mediated by an intrinsic RNA helicase activity of this protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

RNA helicase activity of Escherichia coli SecA protein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Two distinct ATP-binding domains are needed to promote protein export by Escherichia coli SecA ATPase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Use of T7 RNA polymerase to direct expression of cloned genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Helicase motifs: the engine that powers DNA unwinding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A point mutation in Escherichia coli DNA helicase II renders the enzyme nonfunctional in two DNA repair pathways. Evidence for initiation of unwinding from a nick in vivo.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Mutation of a highly conserved arginine in motif IV of Escherichia coli DNA helicase II results in an ATP-binding defect.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Helicase motifs V and VI of the Escherichia coli UvrB protein of the UvrABC endonuclease are essential for the formation of the preincision complex.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA proteins of Bacillus subtilis and Escherichia coli possess homologous amino-terminal ATP-binding domains regulating integration into the plasma membrane

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Endogenous SecA catalyzes preprotein translocation at SecYEG.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Isolation and analysis of dominant secA mutations in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Carboxy-terminal region of Escherichia coli SecA ATPase is important to promote its protein translocation activity in vivo.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structure of UvrB, a DNA helicase adapted for nucleotide excision repair

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structure of a DExx box DNA helicase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Integration of SecA protein into the Escherichia coli inner membrane is regulated by its amino-terminal ATP-binding domain

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Cross-talk between catalytic and regulatory elements in a DEAD motor domain is essential for SecA function

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Characterization of a Mutant Form of SecA That Alleviates a SecY Defect at Low Temperature and Shows a Synthetic Defect with SecY Alteration at High Temperature

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Genetic dissection of SecA: suppressor mutations against the secY205 translocase defect

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Two Independent Mechanisms Down-regulate the Intrinsic SecA ATPase Activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA Is Not Required for Signal Recognition Particle-mediated Targeting and Initial Membrane Insertion of a Nascent Inner Membrane Protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Regulation of a membrane component required for protein secretion in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A partially functional DNA helicase II mutant defective in forming stable binary complexes with ATP and DNA. A role for helicase motif III

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Site-directed mutations in motif VI of Escherichia coli DNA helicase II result in multiple biochemical defects: evidence for the involvement of motif VI in the coupling of ATPase and DNA binding activities via conformational changes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Nucleotide binding activity of SecA homodimer is conformationally regulated by temperature and altered by prlD and azi mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The high affinity ATP binding site modulates the SecA-precursor interaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

21 January 2018

Identifiers

DOI

10.1074/JBC.M104584200

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25 November 2017

1 reference