(Q46421359)

22 December 2017

title

PCR-amplification of GC-rich regions: 'slowdown PCR'. (English)

1 reference

22 December 2017

author name string

Ulrich H Frey

1

1 reference

22 December 2017

Hagen S Bachmann

2

1 reference

22 December 2017

Jürgen Peters

3

1 reference

22 December 2017

Winfried Siffert

4

1 reference

22 December 2017

publication date

1 January 2008

1 reference

22 December 2017

published in

Nature Protocols

1 reference

22 December 2017

volume

3

1 reference

22 December 2017

page(s)

1312-1317

1 reference

22 December 2017

issue

8

1 reference

22 December 2017

https://scigraph.springernature.com/pub.10.1038/nprot.2008.112

exact match

0 references

cites work

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Regulation of gene expression by GC-rich DNA cis-elements

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Successful amplification of extremely GC-rich promoter regions using a novel 'slowdown PCR' technique

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

A novel promoter polymorphism in the human gene GNAS affects binding of transcription factor upstream stimulatory factor 1, Galphas protein expression and body weight regulation.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Characterization of the GNAQ promoter and association of increased Gq expression with cardiac hypertrophy in humans.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

The AA genotype of the regulatory BCL2 promoter polymorphism ( 938C>A) is associated with a favorable outcome in lymph node negative invasive breast cancer patients

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Association of a novel regulatory polymorphism (-938C>A) in the BCL2 gene promoter with disease progression and survival in chronic lymphocytic leukemia

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Comparison of manual and automated nucleic acid extraction from whole-blood samples.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

The highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Optimization of the annealing temperature for DNA amplification in vitro

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Improved PCR method for amplification of GC-rich DNA sequences.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Formamide can dramatically improve the specificity of PCR.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Betaine improves the PCR amplification of GC-rich DNA sequences

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Structure-independent DNA amplification by PCR using 7-deaza-2'-deoxyguanosine

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Dimethyl sulfoxide-mediated primer Tm reduction: a method for analyzing the role of renaturation temperature in the polymerase chain reaction

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Unwinding of double-stranded DNA helix by dehydration

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Incorporation of 7-deaza dGTP during the amplification step in the polymerase chain reaction procedure improves subsequent DNA sequencing

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

'Touchdown' PCR to circumvent spurious priming during gene amplification

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Mutations of the BRAF gene in human cancer

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

7-Deaza-2'-deoxyguanosine allows PCR and sequencing reactions from CpG islands

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Improved free-energy parameters for predictions of RNA duplex stability

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Linkage of the Angiotensinogen Gene to Essential Hypertension

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Deletion polymorphism in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Association of a human G-protein beta3 subunit variant with hypertension

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Mfold web server for nucleic acid folding and hybridization prediction

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

Optimization and troubleshooting in PCR

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

inferred from DOI database lookup

7 January 2021

based on heuristic

High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NPROT.2008.112

1 reference

22 December 2017

PubMed ID

18714299

1 reference

22 December 2017