(Q54389645)

29 May 2018

title

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. (English)

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29 May 2018

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1

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29 May 2018

Hideo Iwai

series ordinal

4

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29 May 2018

author name string

A Sesilja Aranko

series ordinal

2

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29 May 2018

Vytas Raulinaitis

series ordinal

3

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29 May 2018

publication date

4 March 2010

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29 May 2018

published in

Nature Protocols

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29 May 2018

volume

5

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29 May 2018

issue

3

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29 May 2018

page(s)

574-587

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29 May 2018

TROSY and CRINEPT: NMR with large molecular and supramolecular structures in solution.

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Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803

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Protein splicing and related forms of protein autoprocessing

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Semisynthesis of cytotoxic proteins using a modified protein splicing element

21 January 2018

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Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L

21 January 2018

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The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

21 January 2018

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A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins.

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High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product

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Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification

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NMR structure of the R-module: a parallel beta-roll subunit from an Azotobacter vinelandii mannuronan C-5 epimerase.

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Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

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In-Cell NMR Spectroscopy†

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Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

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Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

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Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy

31 March 2020

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Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners

31 March 2020

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A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol

31 March 2020

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Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme

31 March 2020

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A rapid method for the construction of synthetic genes using the polymerase chain reaction

31 March 2020

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PubMed

https://pubmed.ncbi.nlm.nih.gov/20203672

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli

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PubMed

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12 December 2020

based on heuristic

Identifiers

DOI

10.1038/NPROT.2009.240

1 reference

29 May 2018

release_ctlbgg5ahnfj5g4cdzs5a55f5i

Fatcat ID

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/ctlbgg5ahnfj5g4cdzs5a55f5i

mapped directly with Wikidata item

24 November 2022

based on heuristic

1 reference

29 May 2018