(Q54577316)

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A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50- to 100-kb genomic fragments for direct DNA sequencing.

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Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50- to 100-kb genomic fragments for direct DNA sequencing. (English)

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

author name string

Wild J

1

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

Hradecná Z

2

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

Pósfai G

3

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

Szybalski W

4

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Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

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1 November 1996

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Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

179

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Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

181-188

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Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

1

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis.

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Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number

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Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers

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Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.

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The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.

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Identifiers

10.1016/S0378-1119(96)00487-8

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

1 reference

Europe PubMed Central

1 June 2018

http://europepmc.org/abstract/MED/8955645

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