(Q54786646)

3 June 2018

title

Oligonucleotide-directed site-specific mutagenesis of the lac permease of Escherichia coli. (English)

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3 June 2018

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7

Kaback HR

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3 June 2018

Sarkar HK

1

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3 June 2018

Viitanen PV

2

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3 June 2018

Padan E

3

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3 June 2018

Trumble WR

4

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3 June 2018

Poonian MS

5

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3 June 2018

McComas W

6

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3 June 2018

language of work or name

English

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publication date

1 January 1986

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3 June 2018

published in

Methods in Enzymology

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3 June 2018

volume

125

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3 June 2018

page(s)

214-230

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3 June 2018

Efficient site-directed mutagenesis by simultaneous use of two primers

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Oligonucleotide-Directed Mutagenesis Using Plasmid DNA Templates and Two Primers

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Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

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TheLac carrier protein inEscherichia coli

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Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector

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Sequence of the lactose permease gene.

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Mechanism of lactose transport in Escherichia coli membrane vesicles: evidence for the involvement of histidine residue(s) in the response of the lac carrier to the proton electrochemical gradient

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Direct measurement of lactose/proton symport in Escherichia coli membrane vesicles: further evidence for the involvement of histidine residue(s).

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Lactose Carrier Protein of Escherichia coli Structure and Expression of Plasmids Carrying the Y Gene of the lac Operon

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Site-directed mutagenesis of cys148 in the lac carrier protein of Escherichia coli

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7 January 2021

A complementation analysis of the restriction and modification of DNA in Escherichia coli

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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

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The gapped duplex DNA approach to oligonucleotide-directed mutation construction.

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Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis

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Synthesis of deoxyoligonucleotides on a polymer support

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7 January 2021

Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatography

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7 January 2021

The base-pairing specificity of cellulose-pdT9

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7 January 2021

Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.

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Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding

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The use of thin acrylamide gels for DNA sequencing

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The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)

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7 January 2021

A new method for the purification and identification of covalently closed circular DNA molecules

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7 January 2021

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

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7 January 2021

Ethidium-bromide-inhibited restriction endonucleases cleave one strand of circular DNA

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7 January 2021

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

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7 January 2021

cys154 Is important for lac permease activity in Escherichia coli.

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7 January 2021

Functional and immunochemical characterization of a mutant of Escherichia coli energy uncoupled for lactose transport

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7 January 2021

In vitrosite-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency

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7 January 2021

Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments

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7 January 2021

Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd

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7 January 2021

Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli

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7 January 2021

Oligonucleotide directed mutagenesis: Selection of mutants by hemimethylation of GATC-sequences

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7 January 2021

Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo

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7 January 2021

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

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7 January 2021

10.1016/S0076-6879(86)25019-3

Identifiers

DOI

1 reference

3 June 2018

PubMed ID

3520222

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3 June 2018