(Q101104959)
Statements
乙醇溶液对木瓜蛋白酶催化活性的影响 (Chinese)
Effect of Ethanol on Catalytic Activity of Papain (English)
初志战/Chu ZhiZhan
黄卓烈/Huang ZhuoLie*
巫光宏/Wu GuangHong
何平/He Ping
詹福建/Zhan FuJian
丘泰球/Qiu TaiQiu
研究了乙醇溶液对木瓜蛋白酶水解酪蛋白的催化活性及构象的影响.结果表明,木瓜蛋白酶在一定浓度乙醇溶液中水解酪蛋白的活性有显著上升.动力学测定表明木瓜蛋白酶在乙醇溶液中米氏常数(Km)下降.差示光谱显示,在乙醇溶液中木瓜蛋白酶的二级结构发生了变化.荧光发射光谱表明,木瓜蛋白酶在乙醇溶液中发射峰位几乎没移动,但发射强度明显增高.. (Chinese)
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The alterations of papain activity and conformation were studied in condition of pretreatment with ethanol solutions at different concentrations, different pretreatment times, and in condition of various reaction concentrations. (English)
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