(Q101105561)
Statements
Comparison of Two Methods for Extracting Total RNA from Citrus Leaves (English)
柑橘叶片总RNA的两种提取方法比较 (Chinese)
JIANG Juan/姜娟
XUE Jiao-liang/薛皎亮
XIE Ying-ping/谢映平
The two methods for extracting high quality total RNA from the leaves of Citrus reticulata Banco were compared by using RNAplant Reagent (Beijing Tiangen Corporation) and RNAiso Reagent (Japanese TaKaRa Corporation), respectively. (English)
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为了简便、快速提取高质量的柑橘(Citrus reticulata Banco)叶片总RNA,采用TRlzol法,对北京天根公司RNAplant Reagent和日本TaKaRa公司RNAiso Reagent两种RNA提取试剂进行比较并适当改进.结果表明:通过琼脂糖凝胶电泳,使用TaKaRa公司试剂提取的总RNA28S和18S条带清晰,紫外分光光度计检测分析A260/A280为1,820,A260,A230为2.088,RNA的浓度为2.840 μg… (Chinese)
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