(Q101105766)
Statements
Cloning and Prokaryotic Expression of Longan ANS Gene (English)
龙眼花芽ANS基因的克隆与原核表达 (Chinese)
YOU Xiang-rong/游向荣
XU Hong-chuan/许鸿川
LIANG Wen-yu/梁文裕
CHEN Qing-xi/陈清西
ZHENG Shao-quan/郑少泉
The changes in proteome of normal flower and floral reversion buds in longan (Dimocarpus longan Lour.) were studied by using Proteomics method. (English)
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运用蛋白质组学比较龙眼(Dimocarpus hngan Lour.)正常成花和成花逆转花芽的蛋白质组变化,结果表明,ANS蛋白(anthocyanidin synthase)在龙眼成花逆转花芽中下调表达.应用RACE方法克隆ANS蛋白的全长cDNA,长度为1477 bp,包括1个1071 bp的开放阅读框,编码357 bp的氨基酸序列,GenBmk的登录号为FJ479616(GI:218202927).将ANS在大肠杆菌中表达,获得1个约46… (Chinese)
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