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Two-step total gene synthesis method

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scholarly article

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title

Two-step total gene synthesis method (English)

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author name string

Lei Young

series ordinal

1

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Qihan Dong

series ordinal

2

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language of work or name

English

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publication date

2004

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published in

Nucleic Acids Research

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volume

32

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issue

7

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page(s)

e59

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cites work

Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

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24 March 2017

Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences

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24 March 2017

DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis

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24 March 2017

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

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24 March 2017

Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning

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27 September 2017

Molecular computation of solutions to combinatorial problems

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27 September 2017

Studies on polynucleotides, 88. Enzymatic joining of chemically synthesized segments corresponding to the gene for alanine-tRNA

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27 September 2017

FokI method of gene synthesis

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27 September 2017

The use of synthetic genes for the expression of ciliate proteins in heterologous systems

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12 December 2020

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Bridge-overlap-extension PCR method for constructing chimeric genes

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PubMed

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12 December 2020

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Cleavage specificity of bacteriophage T4 endonuclease VII and bacteriophage T7 endonuclease I on synthetic branch migratable holliday junctions

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/15087491

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Dual asymmetric PCR: one-step construction of synthetic genes

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PubMed

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https://pubmed.ncbi.nlm.nih.gov/15087491

retrieved

12 December 2020

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Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases

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Use of a new retrieving adaptor in the cloning of a synthetic human insulin A-chain gene

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https://pubmed.ncbi.nlm.nih.gov/15087491

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

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PubMed publication ID

15087491

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ResearchGate publication ID

8616818

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(Q24563570)

Two-step total gene synthesis method

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