(Q24599175)

English

Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines

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scholarly article

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Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines (English)

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author name string

James F Zawada

1

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Gang Yin

2

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Alexander R Steiner

3

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Junhao Yang

4

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Alpana Naresh

5

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Sushmita M Roy

6

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Daniel S Gold

7

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Henry G Heinsohn

8

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Christopher J Murray

9

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language of work or name

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publication date

July 2011

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Biotechnology and Bioengineering

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108

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7

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1570-8

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Design parameters to control synthetic gene expression in Escherichia coli

1 reference

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Cell-free expression systems for eukaryotic protein production

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PEGylated murine Granulocyte-macrophage colony-stimulating factor: production, purification, and characterization

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Recombinant protein folding and misfolding in Escherichia coli

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Characterizing biological products and assessing comparability following manufacturing changes.

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Recent advances in producing and selecting functional proteins by using cell-free translation

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Combined effects of the signal sequence and the major chaperone proteins on the export of human cytokines in Escherichia coli

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1 reference

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Production and purification of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) from high cell density cultures of Pichia pastoris.

1 reference

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Total amino acid stabilization during cell-free protein synthesis reactions

1 reference

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Development of cell-free protein synthesis platforms for disulfide bonded proteins

1 reference

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Kinetic determinants of high-fidelity tRNA discrimination on the ribosome

1 reference

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Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis

1 reference

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Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis

1 reference

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Efficient synthesis of a disulfide‐containing protein through a batch cell‐free system from wheat germ

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The effect of transcription and translation initiation frequencies on the stochastic fluctuations in prokaryotic gene expression.

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Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source.

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Cell-free translation reconstituted with purified components

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1 reference

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Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis

1 reference

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Efficient and scalable method for scaling up cell free protein synthesis in batch mode.

1 reference

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Redox properties of protein disulfide isomerase (DsbA) from Escherichia coli

1 reference

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Expression of active murine granulocyte-macrophage colony-stimulating factor in an Escherichia coli cell-free system

1 reference

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1 reference

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1 reference

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Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector

1 reference

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12 December 2020

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Identifiers

10.1002/BIT.23103

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Consolidated OpenCitations Corpus – April 2017

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PMC publication ID

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Consolidated OpenCitations Corpus – April 2017

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PubMed publication ID

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Consolidated OpenCitations Corpus – April 2017

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