(Q24803962)

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A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

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title

A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step (English)

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author name string

Rajiv Tyagi

series ordinal

1

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Richard Lai

series ordinal

2

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Ronald G Duggleby

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3

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language of work or name

English

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publication date

26 February 2004

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published in

BMC Biotechnology

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volume

4

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issue

1

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page(s)

2

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cites work

The "megaprimer" method of site-directed mutagenesis

1 reference

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PubMed Central

reference URL

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Systematic characterization of mutations in yeast acetohydroxyacid synthase. Interpretation of herbicide-resistance data

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28 September 2017

Approaches to DNA mutagenesis: an overview

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28 September 2017

Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method

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PubMed Central

reference URL

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28 September 2017

An efficient PCR mutagenesis strategy without gel purification [correction of "purificiation"] step that is amenable to automation

1 reference

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PubMed Central

reference URL

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28 September 2017

A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase

1 reference

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PubMed Central

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28 September 2017

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

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28 September 2017

Nucleotide sequence and in vivo expression of the ilvY and ilvC genes in Escherichia coli K12. Transcription from divergent overlapping promoters.

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30 May 2018

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

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30 May 2018

Megaprimer mutagenesis using very long primers

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PubMed Central

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28 November 2018

Regulatory interactions in Arabidopsis thaliana acetohydroxyacid synthase

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PubMed Central

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28 November 2018

"Megaprimer" method of PCR: increased template concentration improves yield

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https://pubmed.ncbi.nlm.nih.gov/15070414

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An improved PCR-based method for site directed mutagenesis using megaprimers

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15070414

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Purified recombinant Escherichia coli ketol-acid reductoisomerase is unsuitable for use in a coupled assay of acetohydroxyacid synthase activity due to an unexpected side reaction

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15070414

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Creating random mutagenesis libraries using megaprimer PCR of whole plasmid

1 reference

stated in

PubMed

reference URL

https://pubmed.ncbi.nlm.nih.gov/15070414

retrieved

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

DOI

10.1186/1472-6750-4-2

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Dimensions Publication ID

1031660881

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PMC publication ID

385241

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PubMed publication ID

15070414

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ResearchGate publication ID

8632124

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(Q24803962)

A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

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