(Q24814623)

5 July 2017

title

PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency (English)

1 reference

5 July 2017

Oleg Suslov

1

1 reference

5 July 2017

Dennis A Steindler

series ordinal

2

1 reference

5 July 2017

language of work or name

English

0 references

publication date

27 November 2005

1 reference

5 July 2017

published in

Nucleic Acids Research

1 reference

5 July 2017

volume

33

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5 July 2017

issue

20

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5 July 2017

page(s)

e181

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Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling

5 July 2017

cites work

1 reference

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Mfold web server for nucleic acid folding and hybridization prediction

7 April 2017

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

A new mathematical model for relative quantification in real-time RT-PCR

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Properties of the reverse transcription reaction in mRNA quantification

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Kinetic Outlier Detection (KOD) in real-time PCR.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Mutations proximal to the minor groove-binding track of human immunodeficiency virus type 1 reverse transcriptase differentially affect utilization of RNA versus DNA as template

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Improved quantitative real-time RT-PCR for expression profiling of individual cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

The role of template-primer in protection of reverse transcriptase from thermal inactivation

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Polypurine tract primer generation and utilization by Moloney murine leukemia virus reverse transcriptase

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Quantitative reverse transcription strand displacement amplification: quantitation of nucleic acids using an isothermal amplification technique

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Relationship between plus strand DNA synthesis removal of downstream segments of RNA by human immunodeficiency virus, murine leukemia virus and avian myeloblastoma virus reverse transcriptases

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of Rous Sarcoma Virus

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

RNA-dependent DNA polymerase in virions of RNA tumour viruses

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Amplified RNA synthesized from limited quantities of heterogeneous cDNA.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Reverse transcriptase inhibits Taq polymerase activity

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

A DNA-depenent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Real-time RT-PCR: considerations for efficient and sensitive assay design

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expression

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1298932

DNA-directed DNA Polymerase Activity in Oncogenic RNA Viruses

28 November 2018

1 reference

12 December 2020

Reverse transcriptase can block polymerase chain reaction

1 reference

12 December 2020

Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation

1 reference

12 December 2020

Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5'-RACE and primer extension

1 reference

12 December 2020

RNase H and its effects on PCR

1 reference

12 December 2020

Yeast tRNA as carrier in the isolation of microscale RNA for global amplification and expression profiling

1 reference

12 December 2020

Improved indicators for assessing the reliability of detection and quantification by kinetic PCR

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/GNI176

1 reference

release_5rux7idsjja2rdeaffw6cvjflm

5 July 2017

Fatcat ID

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/5rux7idsjja2rdeaffw6cvjflm

24 November 2022

mapped directly with Wikidata item

PMC publication ID

1298932

1 reference

5 July 2017

1 reference