(Q27673494)

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Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline

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scholarly article

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Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline (English)

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structural biology

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condensed matter physics

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Escherichia coli

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based on heuristic

inferred from title

Wesley C Van Voorhis

6

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author name string

Stephen N Hewitt

1

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Ryan Choi

2

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Angela Kelley

3

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Gregory J Crowther

4

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Alberto J Napuli

5

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publication date

1 September 2011

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Acta Crystallographica Section F: Structural Biology Communications

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67

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Pt 9

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1006-9

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Heterologous expression and purification systems for structural proteomics of mammalian membrane proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3169393

20 March 2017

Protein production by auto-induction in high density shaking cultures

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3169393

20 March 2017

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3169393

20 March 2017

Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3169393

20 March 2017

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1 reference

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Ligation-independent cloning of PCR products (LIC-PCR)

1 reference

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7 April 2017

Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

1 reference

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29 September 2017

A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction.

1 reference

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Purification of SUMO conjugating enzymes and kinetic analysis of substrate conjugation.

1 reference

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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein

1 reference

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29 September 2017

Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli

1 reference

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Crystal structures of fusion proteins with large-affinity tags

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3169393

29 September 2017

Protein production: feeding the crystallographers and NMR spectroscopists

1 reference

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Design of high-throughput methods of protein production for structural biology

1 reference

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29 September 2017

Metal-affinity separations: a new dimension in protein processing

1 reference

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29 September 2017

High level expression of a protein precursor for functional studies.

1 reference

https://api.crossref.org/works/10.1107%2FS1744309111022159

21 January 2018

Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes

1 reference

https://api.crossref.org/works/10.1107%2FS1744309111022159

21 January 2018

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

1 reference

https://api.crossref.org/works/10.1107%2FS1744309111022159

21 January 2018

Generating fusions to glutathione S-transferase for protein studies

1 reference

https://pubmed.ncbi.nlm.nih.gov/21904041

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/21904041

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1107/S1744309111022159

1 reference

Consolidated OpenCitations Corpus – April 2017

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OpenCitations bibliographic resource ID

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Consolidated OpenCitations Corpus – April 2017

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