(Q28360902)

English

Identification of the catalytic nucleophile of the Family 31 alpha-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate

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Identification of the catalytic nucleophile of the Family 31 alpha-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate (English)

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Aspergillus niger

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author name string

S S Lee

1

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S He

2

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S G Withers

3

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language of work or name

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publication date

15 October 2001

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Biochemical Journal

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359

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Pt 2

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381-6

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Structures and mechanisms of glycosyl hydrolases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

20 March 2017

New families in the classification of glycosyl hydrolases based on amino acid sequence similarities

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

20 March 2017

Mechanisms of enzymatic glycoside hydrolysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

20 March 2017

Human lysosomal alpha-glucosidase. Characterization of the catalytic site

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

20 March 2017

A classification of glycosyl hydrolases based on amino acid sequence similarities

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

20 March 2017

Quantitative study on anomeric forms of glucose produced by alpha-glucosidases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

20 March 2017

alpha-1,4-glucan lyases producing 1,5-anhydro-D-fructose from starch and glycogen have sequence similarity to alpha-glucosidases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

29 September 2017

Unequivocal identification of Asp-214 as the catalytic nucleophile of Saccharomyces cerevisiae alpha-glucosidase using 5-fluoro glycosyl fluorides

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

29 September 2017

Approaches to labeling and identification of active site residues in glycosidases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

29 September 2017

Glycoside hydrolases: mechanistic information from studies with reversible and irreversible inhibitors

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

29 September 2017

Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

29 September 2017

Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

2 June 2018

Plant alpha-glucosidases of the glycoside hydrolase family 31. Molecular properties, substrate specificity, reaction mechanism, and comparison with family members of different origin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

2 June 2018

Slow binding of D-galactal, a "reversible" inhibitor of bacterial beta-galactosidase.

1 reference

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2 June 2018

Complete amino acid sequence of crystalline alpha-glucosidase from Aspergillus niger

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

2 June 2018

The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

27 November 2018

A catalytic amino acid and primary structure of active site in Aspergillus niger alpha-glucosidase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1222157

27 November 2018

Glycosidase families

1 reference

https://pubmed.ncbi.nlm.nih.gov/11583585

12 December 2020

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Identification of Asp197 as the catalytic nucleophile in the family 38 alpha-mannosidase from bovine kidney lysosomes

1 reference

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12 December 2020

based on heuristic

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Partial amino acid sequences around the essential carboxylate in the active sites of the intestinal sucrase-isomaltase complex

1 reference

https://pubmed.ncbi.nlm.nih.gov/11583585

12 December 2020

based on heuristic

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Identification of Glu340 as the active-site nucleophile in human glucocerebrosidase by use of electrospray tandem mass spectrometry

1 reference

https://pubmed.ncbi.nlm.nih.gov/11583585

12 December 2020

based on heuristic

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Novel structures of N-linked high-mannose type oligosaccharides containing alpha-D-galactofuranosyl linkages in Aspergillus niger alpha-D-glucosidase

1 reference

https://pubmed.ncbi.nlm.nih.gov/11583585

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Chemical modification and amino acid sequence of active site in sugar beet alpha-glucosidase

1 reference

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride

1 reference

https://pubmed.ncbi.nlm.nih.gov/11583585

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Glycosidase mechanisms: anatomy of a finely tuned catalyst

1 reference

https://pubmed.ncbi.nlm.nih.gov/11583585

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

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