(Q30527850)

English

Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration

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scholarly article

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration (English)

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

author name string

Mohab A Al-Hinai

1

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Alan G Fast

2

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Eleftherios T Papoutsakis

3

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

language of work or name

0 references

publication date

14 September 2012

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Applied and Environmental Microbiology

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

78

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

22

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

8112-8121

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Inactivation of σF in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes σE and σG protein expression but does not block solvent formation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Inactivation of σE and σG in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Construction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringens

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Engineering solventogenic clostridia

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Antisense-RNA-mediated decreased synthesis of small, acid-soluble spore proteins leads to decreased resistance of clostridium perfringens spores to moist heat and UV radiation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Restriction for gene insertion within the Lactococcus lactis Ll.LtrB group II intron

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Construction and analysis of chromosomal Clostridium difficile mutants

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Use of targetrons to disrupt essential and nonessential genes in Staphylococcus aureus reveals temperature sensitivity of Ll.LtrB group II intron splicing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Design of antisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

The genome sequence of Clostridium tetani, the causative agent of tetanus disease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Bacterial group II introns and their association with mobile genetic elements

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Counterselectable markers: untapped tools for bacterial genetics and pathogenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Rolling-circle replication of bacterial plasmids.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Chemotherapeutic tumour targeting using clostridial spores

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Cleavage-dependent ligation by the FLP recombinase. Characterization of a mutant FLP protein with an alteration in a catalytic amino acid

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

The Minimum Amount of Homology Required for Homologous Recombination in Mammalian Cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1 reference

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20 June 2018

A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1 reference

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20 June 2018

Gene-directed mutagenesis on the chromosome of Bacillus subtilis 168.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Improved method for high efficiency transformation of intact yeast cells

1 reference

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20 June 2018

Vector construction, transformation, and gene amplification in Clostridium acetobutylicum ATCC 824.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

26 November 2018

A modular system for Clostridium shuttle plasmids

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

26 November 2018

Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

26 November 2018

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

26 November 2018

The α-toxin ofClostridium septicumis essential for virulence

1 reference

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26 November 2018

Development of an integrative vector for the expression of antisense RNA in Clostridium difficile.

1 reference

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26 November 2018

Sequence length required for homologous recombination in Cryptococcus neoformans.

1 reference

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26 November 2018

Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824.

1 reference

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26 November 2018

The ClosTron: a universal gene knock-out system for the genus Clostridium

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

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Effects of ptb knockout on butyric acid fermentation by Clostridium tyrobutyricum

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1128/AEM.02214-12

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

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