(Q30980895)

27 June 2017

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title

Prefractionation techniques in proteome analysis: the mining tools of the third millennium (English)

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27 June 2017

Pier Giorgio Righetti

1

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27 June 2017

Annalisa Castagna

2

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27 June 2017

Paolo Antonioli

3

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27 June 2017

Egisto Boschetti

4

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27 June 2017

1 January 2005

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27 June 2017

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27 June 2017

26

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27 June 2017

297-319

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27 June 2017

2

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Studies on the mechanism of binding of serum albumins to immobilized cibacron blue F3G A.

27 June 2017

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

Proteomics methods for probing molecular mechanisms in signal transduction

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

Has the yo-yo stopped? An assessment of human protein-coding gene number

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

The human plasma proteome: history, character, and diagnostic prospects

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

The human serum proteome: display of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

https://api.crossref.org/works/10.1002%2FELPS.200406189

1 reference

7 January 2021

Zooming-in on the proteome: very narrow-range immobilised pH gradients reveal more protein species and isoforms

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Spot overlapping in two-dimensional polyacrylamide gel electrophoresis separations: a statistical study of complex protein maps.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Spot overlapping in two-dimensional polyacrylamide gel electrophoresis maps: relevance to proteomics

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Strategies for the enrichment and identification of basic proteins in proteome projects

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Unseen proteome: mining below the tip of the iceberg to find low abundance and membrane proteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Prefractionation techniques in proteome analysis.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Methods for fractionation, separation and profiling of proteins and peptides

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Comprehensive proteome analysis by chromatographic protein prefractionation.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Organelle proteomics: implications for subcellular fractionation in proteomics.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteome analysis at the level of subcellular structures

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteomics meets cell biology: the establishment of subcellular proteomes

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteomic analysis of nuclear proteins from proliferative and differentiated human colonic intestinal epithelial cells

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Convenient and versatile subcellular extraction procedure, that facilitates classical protein expression profiling and functional protein analysis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Small heat shock protein p26 associates with nuclear lamins and HSP70 in nuclei and nuclear matrix fractions from stressed cells

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

The establishment of a human liver nuclei two-dimensional electrophoresis reference map

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Directed proteomic analysis of the human nucleolus

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Functional proteomic analysis of human nucleolus

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Mitochondrial proteomics. Undercover in the lipid bilayer

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

The challenges of developing a sound proteomics strategy

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Detection of tumor markers with ProteinChip technology

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Three Biomarkers Identified from Serum Proteomic Analysis for the Detection of Early Stage Ovarian Cancer

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Characterization of the low molecular weight human serum proteome

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteomics of glycoproteins based on affinity selection of glycopeptides from tryptic digests

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Two-dimensional map of Haemophilus influenzae following protein enrichment by heparin chromatography

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Design of protein purification pathways: application to the proteome of Haemophilus influenzae using heparin chromatography

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Enrichment and purification of proteins of Haemophilus influenzae by chromatofocusing

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Enrichment of low-copy-number gene products by hydrophobic interaction chromatography

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Enrichment of human brain proteins by heparin chromatography

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Comprehensive two-dimensional high-performance liquid chromatography for the isolation of overexpressed proteins and proteome mapping

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Enrichment of Escherichia coli proteins by column chromatography on reactive dye columns.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Complexity of sea urchin embryo nuclear proteins that contain basic domains

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A rat brain protein expression map including cytosolic and enriched mitochondrial and microsomal fractions.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Prefractionation of protein samples for proteome analysis using reversed-phase high-performance liquid chromatography

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Analyzing the platelet proteome.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Large-scale analysis of the yeast proteome by multidimensional protein identification technology

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Using immobilized metal affinity chromatography, two-dimensional electrophoresis and mass spectrometry to identify hepatocellular proteins with copper-binding ability

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Histidine-rich peptide selection and quantification in targeted proteomics

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Lectin affinity as an approach to the proteomic analysis of membrane glycoproteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Albumin depletion method for improved plasma glycoprotein analysis by two-dimensional difference gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Microcapillary liquid chromatography/tandem mass spectrometry using alkaline pH mobile phases and positive ion detection

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Protein microarrays for gene expression and antibody screening

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Antibodies in diagnostics - from immunoassays to protein chips

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Protein chips based on recombinant antibody fragments: a highly sensitive approach as detected by mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Protein biochips for differential profiling

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteomics--structure and function

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

New technologies for biomarker analysis of prostate cancer progression: Laser capture microdissection and tissue proteomics

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Use of surface-enhanced laser desorption ionization protein chip system to analyze streptococcal exotoxin B activity secreted by Streptococcus pyogenes

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A protein molecular weight map of ES2 clear cell ovarian carcinoma cells using a two-dimensional liquid separations/mass mapping technique

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Detection and identification of virulence factors in Yersinia pestis using SELDI ProteinChip system

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

The use of seldi proteinchip arrays to monitor production of Alzheimer's betaamyloid in transfected cells.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Tissue-specific microdissection coupled with ProteinChip array technologies: applications in cancer research

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Tagless extraction-retentate chromatography: a new global protein digestion strategy for monitoring differential protein expression

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Understanding gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Enrichment of low-abundance brain proteins by preparative electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Further development of an electroosmotic medium pump system for preparative disk gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Preincubation with cysteine prevents modification of sulfhydryl groups in proteins by unreacted acrylamide in a gel

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Application of free flow electrophoresis to the preparative purification of basic proteins from an E. coli cell extract

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Free-flow electrophoresis in a microfabricated chamber with a micromodule fraction separator. Continuous separation of proteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Free flow-isoelectric focusing of human cellular lysates as sample preparation for protein analysis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Efficient separation and analysis of peroxisomal membrane proteins using free-flow isoelectric focusing

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Preparative free-flow electrofocusing in a vortex-stabilized annulus.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Preparative isoelectric focusing of proteins using binary buffers in a vortex-stabilized, free-flow apparatus

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Free zone electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Recycling isoelectric focusing and isotachophoresis.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Direct ampholyte-free liquid-phase isoelectric peptide focusing: application to the human serum proteome

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Preparative reflux electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Preparative affinity membrane electrophoresis.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Prefractionation of protein samples prior to two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Gradiflow as a prefractionation tool for two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Preparative purification of human monoclonal antibody isoforms in a multi-compartment electrolyser with immobiline membranes

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Amphoteric, isoelectric immobiline membranes for preparative isoelectric focusing

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A turning point in proteome analysis: sample prefractionation via multicompartment electrolyzers with isoelectric membranes

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Prefractionation techniques in proteome analysis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two-dimensional electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Carrier ampholyte-free solution isoelectric focusing as a prefractionation method for the proteomic analysis of complex protein mixtures

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Narrow-band fractionation of proteins from whole cell lysates using isoelectric membrane focusing and nonporous reversed-phase separations

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Alkali-stable high-pI isoelectric membranes for isoelectric trapping separations

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Separation of proteins in a multicompartment electrolyzer with chambers defined by a bed of gel beads.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A miniaturized multichamber solution isoelectric focusing device for separation of protein digests

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Parallel processing in the isoelectric focusing chip

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Protein purification by Off-Gel electrophoresis.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Finite element simulation of Off-Gel trade mark buffering

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Protein fractionation in a multicompartment device using Off-Gel isoelectric focusing.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Sample prefractionation with Sephadex isoelectric focusing prior to narrow pH range two-dimensional gels

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Isoelectric focusing in layers of granulated gels. I. Thin-layer isoelectric focusing of proteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Analysis of membrane proteins by two-dimensional electrophoresis: comparison of the proteins extracted from normal or Plasmodium falciparum-infected erythrocyte ghosts.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Organic solvent extraction as a versatile procedure to identify hydrophobic chloroplast membrane proteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Enrichment of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

An alternative strategy to determine the mitochondrial proteome using sucrose gradient fractionation and 1D PAGE on highly purified human heart mitochondria

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Phase separation of biomolecules in polyoxyethylene glycol nonionic detergents.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteomics Forum '99. Proceedings of a meeting of the German Electrophoresis Society. Munich, Germany, October 25-27, 1999

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Protein pre-fractionation in detergent-polymer aqueous two-phase systems for facilitated proteomic studies of membrane proteins

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

The Early Detection Research Network 2nd Annual Scientific Workshop. 14-16 October 2001. Seattle, Washington, USA. Abstracts

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A new type of synthetic peptide library for identifying ligand-binding activity

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Separation of tumor necrosis factor alpha isoforms by two-dimensional polyacrylamide gel electrophoresis

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A mass spectrometry-based proteomic approach for identification of serine/threonine-phosphorylated proteins by enrichment with phospho-specific antibodies: identification of a novel protein, Frigg, as a protein kinase A substrate

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Proteomics Analysis of Phosphotyrosyl-Proteins in Human Lumbar Cerebrospinal Fluid

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Mass spectrometry for the study of protein-protein interactions.

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Improved immunomatrix methods to detect protein:protein interactions

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

A novel proteomic screen for peptide-protein interactions

1 reference

https://api.crossref.org/works/10.1002%2FELPS.200406189

7 January 2021

Identifiers

DOI

10.1002/ELPS.200406189

1 reference

27 June 2017

1 reference