(Q31926669)

5 July 2017

title

Enhancement of stability and activity of phospholipase A(1) in organic solvents by directed evolution (English)

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5 July 2017

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J K Song

1

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5 July 2017

J S Rhee

series ordinal

2

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5 July 2017

language of work or name

English

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publication date

1 June 2001

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Biochimica et Biophysica Acta

5 July 2017

published in

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5 July 2017

volume

1547

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5 July 2017

issue

2

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5 July 2017

page(s)

370-378

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Biocatalysis in nonaqueous solvents

5 July 2017

cites work

1 reference

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Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtilisin E in polar organic media

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Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E.

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Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

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Randomization of genes by PCR mutagenesis

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DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

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Chloroquine treatment does not cause phospholipid storage by depleting rat liver lysosomes of acid phospholipase A.

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Elevated levels of cellular and extracellular phospholipases from pathogenic Naegleria fowleri

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Allergens in hymenoptera venom. XXVI: The complete amino acid sequences of two vespid venom phospholipases

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Screening for and characterization of phospholipase A1 hypersecretory mutants of Tetrahymena thermophila

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Cloning of a phosphatidic acid-preferring phospholipase A1 from bovine testis.

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Lysosomal phospholipase a activities of rat ovarian tissue

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Secretion of phospholipase A1 by bone marrow-derived macrophages.

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Molecular cloning and expression of the gene encoding a phospholipase A1 from Aspergillus oryzae

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7 January 2021

Simultaneous enhancement of thermostability and catalytic activity of phospholipase A(1) by evolutionary molecular engineering

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7 January 2021

Optimization of DNA shuffling for high fidelity recombination

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7 January 2021

Directed evolution of a thermostable esterase.

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7 January 2021

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

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7 January 2021

Stabilization of substilisin E in organic solvents by site-directed mutagenesis

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Engineering enzymes for non-aqueous solvents

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7 January 2021

10.1016/S0167-4838(01)00204-7

Identifiers

DOI

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5 July 2017

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