(Q33201151)

23 July 2017

Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop (English)

1 reference

23 July 2017

1 reference

Melissa L Geddie

1

1 reference

PubMed ID

15069062

23 July 2017

Ichiro Matsumura

series ordinal

2

1 reference

PubMed ID

15069062

23 July 2017

language of work or name

English

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publication date

6 April 2004

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Journal of Biological Chemistry

23 July 2017

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23 July 2017

279

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23 July 2017

26462-26468

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23 July 2017

25

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In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates

23 July 2017

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Enzyme recruitment in evolution of new function

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Some aspects of the evolution of metabolic pathways.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Glycoside hydrolases and glycosyltransferases: families and functional modules

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Structural and sequence-based classification of glycoside hydrolases

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Structure of human beta-glucuronidase reveals candidate lysosomal targeting and active-site motifs

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

A comparison of directed evolution approaches using the beta-glucuronidase model system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Directed enzyme evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Directed evolution of enzymes and pathways for industrial biocatalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Directed evolution of single proteins, metabolic pathways, and viruses

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Engineering enzyme specificity.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Directed Evolution and Biocatalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Directed evolution of a glutaryl acylase into an adipyl acylase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Growth factor engineering by degenerate homoduplex gene family recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Guided evolution of enzymes with new substrate specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Effective amplification of long targets from cloned inserts and human genomic DNA

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

High efficiency transformation of E. coli by high voltage electroporation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

High throughput microplate screens for directed protein evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase: kinetic studies

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Increased antibody expression from Escherichia coli through wobble-base library mutagenesis by enzymatic inverse PCR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Generation of entire human papillomavirus genomes by long PCR: frequency of errors produced during amplification

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Stimulation and suppression of PCR-mediated recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Factors affecting PCR-mediated recombination.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

A structural view of the action of Escherichia coli (lacZ) beta-galactosidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Computational method to reduce the search space for directed protein evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Computational design of receptor and sensor proteins with novel functions

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Combining computational and experimental screening for rapid optimization of protein properties

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M401447200

21 January 2018

Identifiers

DOI

10.1074/JBC.M401447200

1 reference

23 July 2017

1 reference

PubMed ID

15069062