(Q33366975)

26 July 2017

title

Touchdown PCR for increased specificity and sensitivity in PCR amplification (English)

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26 July 2017

2

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26 July 2017

Darren J Korbie

1

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26 July 2017

publication date

1 January 2008

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26 July 2017

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26 July 2017

volume

3

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26 July 2017

issue

9

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26 July 2017

page(s)

1452-1456

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Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia

26 July 2017

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

'Touchdown' PCR to circumvent spurious priming during gene amplification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Quantification of mRNA using real-time RT-PCR

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Comparison of different melting temperature calculation methods for short DNA sequences.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

OligoCalc: an online oligonucleotide properties calculator.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Stability of ribonucleic acid double-stranded helices

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Use of degenerate primers and touchdown PCR to amplify a halogenase gene fragment from Streptomyces venezuelae ISP5230.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Mapping transposon insertion sites by touchdown PCR and hybrid degenerate primers

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Analysis of circadian liver gene expression by ADDER, a highly sensitive method for the display of differentially expressed mRNAs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Combining restriction digestion and touchdown PCR permits detection of trace isoforms of histamine H3 receptor

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Type-specific amplification of viral DNA using touchdown and hot start PCR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

History and principles of conductive media for standard DNA electrophoresis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Molecular fingerprinting of isoniazid-resistant Mycobacterium tuberculosis isolates from chest diseases hospital in Kuwait

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Rapid detection of ethambutol-resistant Mycobacterium tuberculosis strains by PCR-RFLP targeting embB codons 306 and 497 and iniA codon 501 mutations.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

The occurrence of rare rpoB mutations in rifampicin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Species spectrum of nontuberculous mycobacteria isolated from clinical specimens in Kuwait

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Cross-reaction between mammalian cell entry (Mce) proteins of Mycobacterium tuberculosis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Mammalian cell-entry proteins encoded by the mce3 operon of Mycobacterium tuberculosis are expressed during natural infection in humans

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Enzymatic amplification of DNA by PCR: standard procedures and optimization

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Improved nearest-neighbor parameters for predicting DNA duplex stability

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Multistep-touchdown vectorette-PCR--a rapid technique for the identification of IVS in genes

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

The use of "touchdown" polymerase chain reaction increases the sensitivity and specificity of t(11;14)(q13;q32) detection in patients with mantle cell lymphoma

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Use of degenerate primers and touchdown PCR for construction of cDNA libraries

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Preparation of genomic DNA from mammalian tissue

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.133

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NPROT.2008.133

1 reference

Dimensions Publication ID

26 July 2017

1011641348

0 references

1 reference