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Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing.
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Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
title
Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing
(English)
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
author
Manuel Ares
series ordinal
2
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stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
author name string
Rhonda Perriman
series ordinal
1
1 reference
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Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
publication date
1 May 2010
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Europe PubMed Central
PMCID
2872779
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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
number of pages
12
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based on heuristic
inferred from page(s)
published in
Molecular Cell
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Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
volume
38
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
page(s)
416-427
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
issue
3
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
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Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps
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X-ray Structures of U2 snRNA−Branchpoint Duplexes Containing Conserved Pseudouridines † ‡
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The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing
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U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing
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Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing
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U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes
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28 July 2018
Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism
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28 July 2018
Modified nucleotides at the 5' end of human U2 snRNA are required for spliceosomal E-complex formation
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ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA
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28 July 2018
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine
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28 July 2018
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure
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28 July 2018
A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly
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28 July 2018
Multiple roles for the yeast SUB2/yUAP56 gene in splicing
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Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56
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28 July 2018
Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
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28 July 2018
ATP can be dispensable for prespliceosome formation in yeast
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28 July 2018
An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p
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28 July 2018
CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif
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28 July 2018
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28 July 2018
The structure of an essential splicing element: stem loop IIa from yeast U2 snRNA
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28 July 2018
The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA.
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PubMed Central
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28 July 2018
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28 July 2018
Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits
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28 July 2018
Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1
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28 July 2018
A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing
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PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
Mutations in an essential U2 small nuclear RNA structure cause cold-sensitive U2 small nuclear ribonucleoprotein function by favoring competing alternative U2 RNA structures
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase
1 reference
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PubMed Central
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28 July 2018
Randomization-selection analysis of snRNAs in vivo: evidence for a tertiary interaction in the spliceosome
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28 July 2018
Rearrangement of snRNA structure during assembly and function of the spliceosome
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
Early commitment of yeast pre-mRNA to the spliceosome pathway
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
A compensatory base change in human U2 snRNA can suppress a branch site mutation
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
In vitro reconstitution of functional yeast U2 snRNPs
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28 July 2018
Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
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28 July 2018
Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes
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28 July 2018
A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing
1 reference
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element
1 reference
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PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
Mutational analysis of the yeast U2 snRNA suggests a structural similarity to the catalytic core of group I introns
1 reference
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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28 July 2018
DExD/H-box Prp5 protein is in the spliceosome during most of the splicing cycle
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeast
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Solution and crystal structures of mRNA exporter Dbp5p and its interaction with nucleotides
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29 October 2018
Structural basis for exon recognition by a group II intron
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PubMed Central
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29 October 2018
Competition between the ATPase Prp5 and branch region-U2 snRNA pairing modulates the fidelity of spliceosome assembly
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Crystal structure and nucleotide binding of the Thermus thermophilus RNA helicase Hera N-terminal domain
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29 October 2018
Repositioning of the reaction intermediate within the catalytic center of the spliceosome.
1 reference
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PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants.
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPase
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA.
1 reference
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29 October 2018
A spontaneous duplication in U6 spliceosomal RNA uncouples the early and late functions of the ACAGA element in vivo.
1 reference
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PubMed Central
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29 October 2018
A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates
1 reference
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29 October 2018
The RNA primer promoter, as defined in vitro, is essential for pMB1 plasmid replication in vivo
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
U2–U6 RNA folding reveals a group II intron-like domain and a four-helix junction
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2872779
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29 October 2018
Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/20471947
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Comparison of transfer ribonucleic acid structures using cobra venom and S1 endonucleases
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/20471947
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/20471947
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
The 5' end of U2 snRNA is in close proximity to U1 and functional sites of the pre-mRNA in early spliceosomal complexes
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/20471947
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Identifiers
DOI
10.1016/J.MOLCEL.2010.02.036
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
PMCID
2872779
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
PubMed ID
20471947
1 reference
stated in
Europe PubMed Central
PMCID
2872779
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:20471947%20AND%20SRC:MED&resulttype=core&format=json
retrieved
2 February 2020
ResearchGate publication ID
44601171
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