(Q33864043)

29 July 2017

title

Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification (English)

1 reference

29 July 2017

Shuang Zhang

1

1 reference

29 July 2017

Zhi Hui Wang

series ordinal

2

1 reference

29 July 2017

Guo Qiang Chen

series ordinal

3

1 reference

29 July 2017

publication date

10 May 2010

1 reference

29 July 2017

published in

Microbial Cell Factories

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29 July 2017

volume

9

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29 July 2017

page(s)

28

1 reference

https://scigraph.springernature.com/pub.10.1186/1475-2859-9-28

29 July 2017

exact match

0 references

cites work

Autoregulator protein PhaR for biosynthesis of polyhydroxybutyrate [P(3HB)] possibly has two separate domains that bind to the target DNA and P(3HB): Functional mapping of amino acid residues responsible for DNA binding

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Protein splicing in cis and in trans

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

A repressor protein, PhaR, regulates polyhydroxyalkanoate (PHA) synthesis via its direct interaction with PHA

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Intein-mediated ligation and cyclization of expressed proteins

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Semisynthesis of cytotoxic proteins using a modified protein splicing element

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Control of protein splicing by intein fragment reassembly.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Production and purification of a recombinant elastomeric polypeptide, G-(VPGVG)19-VPGV, from Escherichia coli

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

5 July 2018

1 reference

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CBM21 starch-binding domain: a new purification tag for recombinant protein engineering.

5 July 2018

1 reference

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A novel self-cleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

About the cover: The Z basic protein tag.

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli.

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

High-throughput purification of hexahistidine-tagged proteins expressed in E. coli

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Simple bioseparations using self-cleaving elastin-like polypeptide tags.

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli.

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Synthetic two-piece and three-piece split inteins for protein trans-splicing

29 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2873406

Purification of recombinant proteins by fusion with thermally-responsive polypeptides

29 October 2018

1 reference

12 December 2020

Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor

1 reference

12 December 2020

Intein-mediated rapid purification of Cre recombinase

1 reference

12 December 2020

A method for the amidation of recombinant peptides expressed as intein fusion proteins in Escherichia coli

1 reference

12 December 2020

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

1 reference

12 December 2020

The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins

1 reference

12 December 2020

Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein

1 reference

12 December 2020

Nonspecific hydrophobic interactions of a repressor protein, PhaR, with poly[(R)-3-hydroxybutyrate] film studied with a quartz crystal microbalance

1 reference

12 December 2020

Fusions to self-splicing inteins for protein purification

1 reference

12 December 2020

Identifiers

DOI

10.1186/1475-2859-9-28

1 reference

Dimensions Publication ID

29 July 2017

1 reference

https://scigraph.springernature.com/pub.10.1186/1475-2859-9-28

9 September 2019

1 reference

29 July 2017

1 reference