(Q33996956)

30 July 2017

1 reference

title

Reporter gene vectors and assays (English)

1 reference

30 July 2017

Schenborn E

1

1 reference

30 July 2017

Groskreutz D

series ordinal

2

1 reference

30 July 2017

publication date

1 November 1999

1 reference

30 July 2017

published in

Molecular Biotechnology

1 reference

30 July 2017

volume

13

1 reference

30 July 2017

issue

1

1 reference

30 July 2017

page(s)

29-44

1 reference

The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing

30 July 2017

cites work

1 reference

12 December 2020

Amplified assay of alkaline phosphatase using flavin-adenine dinucleotide phosphate as substrate

1 reference

12 December 2020

Chemiluminescent and bioluminescent reporter gene assays

1 reference

12 December 2020

Use of bacterial and firefly luciferases as reporter genes in DEAE-dextran-mediated transfection of mammalian cells

1 reference

12 December 2020

The molecular structure of green fluorescent protein

1 reference

12 December 2020

A simple assay for DNA transfection by incubation of the cells in culture dishes with substrates for beta-galactosidase

1 reference

12 December 2020

Low-light image analysis of transgenic organisms using bacterial luciferase as a marker

1 reference

12 December 2020

Selective production of transgenic mice using green fluorescent protein as a marker

1 reference

12 December 2020

A novel genetic system to detect protein-protein interactions

1 reference

12 December 2020

Mammalian beta-glucuronidase: genetics, molecular biology, and cell biology

1 reference

12 December 2020

GFP illuminates the cytoskeleton.

1 reference

12 December 2020

The development of beta-lactamase as a highly versatile genetic reporter for eukaryotic cells

1 reference

12 December 2020

Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells

1 reference

12 December 2020

Introns in the 3'-untranslated region can inhibit chimeric CAT and beta-galactosidase gene expression

1 reference

12 December 2020

Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9

1 reference

12 December 2020

Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin

1 reference

12 December 2020

Green fluorescent protein as a marker for gene expression

1 reference

12 December 2020

Selective inactivation of eukaryotic beta-galactosidase in assays for inhibitors of HIV-1 TAT using bacterial beta-galactosidase as a reporter enzyme

1 reference

12 December 2020

Translational potentiation of messenger RNA with secondary structure in Xenopus

1 reference

12 December 2020

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest

1 reference

12 December 2020

Introns increase transcriptional efficiency in transgenic mice

1 reference

12 December 2020

Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos

1 reference

12 December 2020

Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells

1 reference

12 December 2020

Improved green fluorescent protein by molecular evolution using DNA shuffling

1 reference

12 December 2020

A novel technique for in-vivo assay of viral regulatory regions in genomes of animal RNA viruses

1 reference

12 December 2020

Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli

1 reference

12 December 2020

Poly(A), poly(A) binding protein and the regulation of mRNA stability

1 reference

12 December 2020

Firefly luciferase gene: structure and expression in mammalian cells

1 reference

12 December 2020

Comparison of intron-dependent and intron-independent gene expression

1 reference

12 December 2020

Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses.

1 reference

12 December 2020

Whole animal cell sorting of Drosophila embryos.

1 reference

12 December 2020

Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis.

1 reference

12 December 2020

Alkaline phosphatase as a reporter enzyme

1 reference

12 December 2020

Use of a fluorescent chloramphenicol derivative as a substrate for CAT assays

1 reference

12 December 2020

The green fluorescent protein

1 reference

12 December 2020

A sensitive promoter assay based on the transcriptional activator Tat of the HIV-1 virus

1 reference

12 December 2020

Influences of mRNA secondary structure on initiation by eukaryotic ribosomes

1 reference

12 December 2020

A novel transgenic mouse model for the in vivo evaluation of anti-human immunodeficiency virus type 1 drugs

1 reference

12 December 2020

Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs

1 reference

12 December 2020

A sensitive method for the detection of beta-galactosidase in transfected mammalian cells

1 reference

12 December 2020

Characterization of lipid DNA interactions. I. Destabilization of bound lipids and DNA dissociation

1 reference

12 December 2020

Expression of a human placental alkaline phosphatase gene in transfected cells: use as a reporter for studies of gene expression

1 reference

12 December 2020

Primary structure of the Aequorea victoria green-fluorescent protein

1 reference

12 December 2020

Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells

1 reference

12 December 2020

Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins

1 reference

12 December 2020

Kinetic determination of cellular LacZ expression

1 reference

12 December 2020

57 Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria

1 reference

12 December 2020

1 reference

12 December 2020

Structure of chloramphenicol acetyltransferase at 1.75-A resolution

1 reference

12 December 2020

Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells

1 reference

12 December 2020

A polymerase chain reaction-based method for detection and quantification of reporter gene expression in transient transfection assays

1 reference

12 December 2020

Influence of the DNA complexation medium on the transfection efficiency of lipospermine/DNA particles

1 reference

12 December 2020

A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: Application to quantitation of β-galactosidase in lacZ-transfected cells

1 reference

12 December 2020

Human growth hormone as a reporter gene in regulation studies employing transient gene expression

1 reference

12 December 2020

[72] Identification of regulatory elements of cloned genes with functional assays

1 reference

12 December 2020

Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter

1 reference

12 December 2020

Green fluorescent protein as a signal for protein-protein interactions

1 reference

12 December 2020

GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants

1 reference

12 December 2020

Variation in enzymatic transient gene expression assays

1 reference

12 December 2020

High sensitivity for color mutants in lacZ plasmid-based transgenic mice, as detected by positive selection

1 reference

12 December 2020

Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo

1 reference

12 December 2020

Heterologous introns can enhance expression of transgenes in mice

1 reference

12 December 2020

Initiation of translation at internal AUG codons in mammalian cells

1 reference

12 December 2020

Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein

1 reference

12 December 2020

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells

1 reference

12 December 2020

smg/rap1/Krev-1 p21s inhibit the signal pathway to the c-fos promoter/enhancer from c-Ki-ras p21 but not from c-raf-1 kinase in NIH3T3 cells

1 reference

12 December 2020

Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA

1 reference

12 December 2020

The scanning model for translation: an update

1 reference

12 December 2020

Novel lacZ-based recombination vectors for mammalian cells

1 reference

12 December 2020

A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells

1 reference

12 December 2020

A genome-wide functional assay of signal transduction in living mammalian cells

1 reference

12 December 2020

Monitoring foreign gene expression by a human adenovirus-based vector using the firefly luciferase gene as a reporter

1 reference

12 December 2020

Transgenic animals with inducible, targeted gene expression in brain

1 reference

12 December 2020

Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy

1 reference

12 December 2020

Crystal structure of the Aequorea victoria green fluorescent protein

1 reference

12 December 2020

Do the poly(A) tail and 3' untranslated region control mRNA translation?

1 reference

12 December 2020

Functional testing of human dopamine D1 and D5 receptors expressed in stable cAMP-responsive luciferase reporter cell lines

1 reference

12 December 2020

GTPase-activating protein SH2-SH3 domains induce gene expression in a Ras-dependent fashion

1 reference

12 December 2020

A high capacity assay for inhibitors of human papillomavirus DNA replication.

1 reference

12 December 2020

Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells

1 reference

12 December 2020

In vitro and in vivo targeting of gene expression to melanoma cells

1 reference

12 December 2020

High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.

1 reference

12 December 2020

Modulation of firefly luciferase stability and impact on studies of gene regulation

1 reference

12 December 2020

Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes

1 reference

12 December 2020

Photinus pyralis luciferase: vectors that contain a modified luc coding sequence allowing convenient transfer into other systems

1 reference

12 December 2020

Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer

1 reference

12 December 2020

Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322

1 reference

12 December 2020

Reporter genes: application to the study of mammalian gene transcription

1 reference

12 December 2020

Synthesis and characterization of luciferin derivatives for use in bioluminescence enhanced enzyme immunoassays. New ultrasensitive detection systems for enzyme immunoassays, I

1 reference

12 December 2020

A generic intron increases gene expression in transgenic mice

1 reference

12 December 2020

Evaluation of a genetically engineered cell line and a histochemical beta-galactosidase assay to detect herpes simplex virus in clinical specimens

1 reference

12 December 2020

The 5' untranslated sequence of the c-sis/platelet-derived growth factor 2 transcript is a potent translational inhibitor

1 reference

12 December 2020

Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences

1 reference

12 December 2020

Eukaryotic regulatory elements lurking in plasmid DNA: the activator protein-1 site in pUC.

1 reference

12 December 2020

Unidirectional digestion with exonuclease III in DNA sequence analysis

1 reference

12 December 2020

Colon epithelium can be transiently transfected with liposomes, calcium phosphate precipitation and DEAE dextran in vivo

1 reference

12 December 2020

Use of alkaline phosphatase as a reporter polypeptide to study the role of the subtilin leader segment and the SpaT transporter in the posttranslational modifications and secretion of subtilin in Bacillus subtilis 168.

1 reference

12 December 2020

Post-transcriptional regulation of the human transforming growth factor-beta 1 gene.

1 reference

12 December 2020

Differential effects of polyadenylation regions on gene expression in mammalian cells

1 reference

12 December 2020

Understanding, improving and using green fluorescent proteins

1 reference

12 December 2020

pSV00CAT: low background CAT plasmid

1 reference

12 December 2020

Expression vectors encoding human growth hormone (hGH) controlled by human muscle-specific promoters: prospects for regulated production of hGH delivered by myoblast transfer or intravenous injection

1 reference

12 December 2020

The beta-gal interferon assay: a new, precise and sensitive method

1 reference

12 December 2020

A simple phase-extraction assay for chloramphenicol acyltransferase activity

1 reference

12 December 2020

A comparison of different promoter, enhancer, and cell type combinations in transient transfections

1 reference

12 December 2020

Identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6

1 reference

12 December 2020

Reporter constructs with low background activity utilizing the cat gene

1 reference

12 December 2020

Identifiers

DOI

10.1385/MB:13:1:29

1 reference

Dimensions Publication ID

30 July 2017

1043018232

0 references

1 reference