(Q34024340)

30 July 2017

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title

Protein splicing and its applications. (English)

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30 July 2017

Perler FB

1

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30 July 2017

Adam E

series ordinal

2

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30 July 2017

publication date

1 August 2000

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Current Opinion in Biotechnology

30 July 2017

published in

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30 July 2017

volume

11

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30 July 2017

issue

4

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30 July 2017

page(s)

377-383

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InBase, the Intein Database

30 July 2017

cites work

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7 January 2021

Semisynthesis of cytotoxic proteins using a modified protein splicing element

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7 January 2021

Expressed protein ligation: a general method for protein engineering

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7 January 2021

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

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7 January 2021

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

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7 January 2021

The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins

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7 January 2021

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

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7 January 2021

Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein

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7 January 2021

A genetic system yields self-cleaving inteins for bioseparations

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7 January 2021

Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI

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7 January 2021

Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein

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7 January 2021

Improved segmental isotope labeling of proteins and application to a larger protein

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7 January 2021

NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation

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7 January 2021

Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein

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7 January 2021

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Protein splicing in vitro with a semisynthetic two-component minimal intein

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Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803

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7 January 2021

A natural example of protein trans-splicing

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7 January 2021

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7 January 2021

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Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs

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7 January 2021

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7 January 2021

NMR spectroscopy of large molecules and multimolecular assemblies in solution.

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7 January 2021

Production of cyclic peptides and proteins in vivo

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7 January 2021

Chemical protein synthesis

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7 January 2021

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7 January 2021

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7 January 2021

Expressed protein ligation, a novel method for studying protein-protein interactions in transcription

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7 January 2021

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7 January 2021

Expressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17

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7 January 2021

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis

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7 January 2021

Use of benzyl mercaptan for direct preparation of long polypeptide benzylthio esters as substrates of subtiligase

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7 January 2021

Methionine ligation strategy in the biomimetic synthesis of parathyroid hormones

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7 January 2021

Identification of a virus intein and a possible variation in the protein-splicing reaction

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7 January 2021

Structural insights into the protein splicing mechanism of PI-SceI

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https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900113-0

7 January 2021

Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing

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https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900113-0

7 January 2021

10.1016/S0958-1669(00)00113-0

Identifiers

DOI

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30 July 2017

1 reference