(Q34066544)

30 July 2017

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title

New developments in isotope labeling strategies for protein solution NMR spectroscopy (English)

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30 July 2017

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Goto NK

1

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30 July 2017

Kay LE

series ordinal

2

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30 July 2017

publication date

1 October 2000

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Current Opinion in Structural Biology

30 July 2017

published in

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30 July 2017

volume

10

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30 July 2017

issue

5

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30 July 2017

page(s)

585-592

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Structures of larger proteins in solution: three- and four-dimensional heteronuclear NMR spectroscopy

30 July 2017

cites work

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7 January 2021

The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins

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7 January 2021

Carbon-13 line narrowing by deuterium decoupling in deuterium/carbon-13/nitrogen-15 enriched proteins. Application to triple resonance 4D J connectivity of sequential amides

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7 January 2021

A Suite of Triple Resonance NMR Experiments for the Backbone Assignment of 15N, 13C, 2H Labeled Proteins with High Sensitivity

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7 January 2021

NMR sequential assignment of Escherichia coli thioredoxin utilizing random fractional deuteriation

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7 January 2021

NMR signal assignments of amide protons in the alpha-helical domains of staphylococcal nuclease

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7 January 2021

Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance

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7 January 2021

Solution structure of the 30 kDa N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system by multidimensional NMR

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7 January 2021

Structure of Cdc42 in complex with the GTPase-binding domain of the 'Wiskott-Aldrich syndrome' protein

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7 January 2021

TROSY in triple-resonance experiments: new perspectives for sequential NMR assignment of large proteins

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7 January 2021

Assignment of15N,13Cα,13Cβ, and HN Resonances in an15N,13C,2H Labeled 64 kDa Trp Repressor−Operator Complex Using Triple-Resonance NMR Spectroscopy and2H-Decoupling

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7 January 2021

Solution NMR Studies of a 42 KDaEscherichiaColiMaltose Binding Protein/β-Cyclodextrin Complex: Chemical Shift Assignments and Analysis

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7 January 2021

Transverse Relaxation-Optimized Spectroscopy (TROSY) for NMR Studies of Aromatic Spin Systems in13C-Labeled Proteins

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7 January 2021

Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution

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7 January 2021

Selective Methyl Group Protonation of Perdeuterated Proteins

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7 January 2021

Surface, subunit interfaces and interior of oligomeric proteins

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7 January 2021

A robust and cost-effective method for the production of Val, Leu, Ile (delta 1) methyl-protonated 15N-, 13C-, 2H-labeled proteins

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7 January 2021

Production and Incorporation of15N,13C,2H (1H-δ1 Methyl) Isoleucine into Proteins for Multidimensional NMR Studies

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7 January 2021

Global folds of proteins with low densities of NOEs using residual dipolar couplings: application to the 370-residue maltodextrin-binding protein

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7 January 2021

Structure of the cell-adhesion fragment of intimin from enteropathogenic Escherichia coli.

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7 January 2021

The Ig fold of the core binding factor alpha Runt domain is a member of a family of structurally and functionally related Ig-fold DNA-binding domains

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7 January 2021

NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio

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7 January 2021

Structure and mutagenesis of the Dbl homology domain

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7 January 2021

Characterization of long-range structure in the denatured state of staphylococcal nuclease. II. Distance restraints from paramagnetic relaxation and calculation of an ensemble of structures

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7 January 2021

Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited nuclear overhauser effect data

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https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Protein global fold determination using site-directed spin and isotope labeling

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7 January 2021

Measurement of 3hJNC′ connectivities across hydrogen bonds in a 30 kDa protein

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7 January 2021

Application of amino acid type-specific 1H- and 14N-labeling in a 2H-, 15N-labeled background to a 47 kDa homodimer: potential for NMR structure determination of large proteins.

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7 January 2021

The 3D NOESY-[(1)H,(15)N,(1)H]-ZQ-TROSY NMR experiment with diagonal peak suppression

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7 January 2021

Pilus chaperone FimC-adhesin FimH interactions mapped by TROSY-NMR

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7 January 2021

A 4D TROSY-based pulse scheme for correlating 1HNi,15Ni,13Calphai,13C'i-1 chemical shifts in high molecular weight, 15N,13C, 2H labeled proteins

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https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution

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https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Improved segmental isotope labeling of proteins and application to a larger protein

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https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation

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7 January 2021

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

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7 January 2021

Chemical protein synthesis

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7 January 2021

A study of protein side-chain dynamics from new 2H auto-correlation and 13C cross-correlation NMR experiments: application to the N-terminal SH3 domain from drk.

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7 January 2021

Correlation between binding and dynamics at SH2 domain interfaces

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7 January 2021

Redistribution and loss of side chain entropy upon formation of a calmodulin-peptide complex

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7 January 2021

Backbone and methyl dynamics of the regulatory domain of troponin C: anisotropic rotational diffusion and contribution of conformational entropy to calcium affinity.

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7 January 2021

Changes in side-chain and backbone dynamics identify determinants of specificity in RNA recognition by human U1A protein

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7 January 2021

Effect of hydrophobic core packing on sidechain dynamics

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7 January 2021

Improved labeling strategy for 13C relaxation measurements of methyl groups in proteins.

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7 January 2021

Heterologous protein expression in the methylotrophic yeast Pichia pastoris

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7 January 2021

Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation

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7 January 2021

Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1

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7 January 2021

Use of Pichia pastoris for the expression, purification, and characterization of rat calretinin "EF-hand" domains.

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7 January 2021

Isotopically labeled bovine beta-lactoglobulin for NMR studies expressed in Pichia pastoris

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https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris.

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

The solution structure of type II antifreeze protein reveals a new member of the lectin family

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts.

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

1H resonance assignments and secondary structure of the 13.6 kDa glycosylated adhesion domain of human CD2

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https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

A practical method for uniform isotopic labeling of recombinant proteins in mammalian cells

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Expression of human chorionic gonadotropin uniformly labeled with NMR isotopes in Chinese hamster ovary cells: an advance toward rapid determination of glycoprotein structures

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Cell-free production and stable-isotope labeling of milligram quantities of proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

tRNA-mediated protein engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0959-440X%2800%2900135-4

7 January 2021

10.1016/S0959-440X(00)00135-4

Identifiers

DOI

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30 July 2017

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