(Q34228979)

31 July 2017

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title

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. (English)

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31 July 2017

Richard J Jenny

1

1 reference

PubMed ID

12963335

31 July 2017

Kenneth G Mann

series ordinal

2

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PubMed ID

12963335

31 July 2017

Roger L Lundblad

series ordinal

3

1 reference

31 July 2017

1 September 2003

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Protein Expression and Purification

31 July 2017

1 reference

31 July 2017

31

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31 July 2017

1-11

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31 July 2017

1

1 reference

Improved expression characteristics of single-chain Fv fragments when fused downstream of the Escherichia coli maltose-binding protein or upstream of a single immunoglobulin-constant domain

31 July 2017

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Production of a soluble and active MBP-scFv fusion: favorable effect of the leaky tolR strain

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Evaluation of cell surface antigens as potential targets for recombinant tumor toxins

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression and purification of recombinant immunotoxin--a fusion protein stabilizes a single-chain Fv (scFv) in denaturing condition

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Fusion tails for the recovery and purification of recombinant proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Influence of the Histidine Tail on the Structure and Activity of Recombinant Chlorocatechol 1,2-Dioxygenase

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Amino acid sequence studies on factor XIII and the peptide released during its activation by thrombin

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

The hydrolysis of chemically modified proteins by Factor Xa and thrombin

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Thrombin specificity. Selective cleavage of antibody light chains at the joints of variable with joining regions and joining with constant regions

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Overexpression, oxidative refolding, and zinc binding of recombinant forms of the murine S100 protein MRP14 (S100A9)

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

The proteins encoded by the rbs operon of Escherichia coli: II. Use of chimeric protein constructs to isolate and characterize RbsC.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Molecular cloning and nucleotide sequence of cDNA encoding rat kidney long-chain L-2-hydroxy acid oxidase. Expression of the catalytically active recombinant protein as a chimaera

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Cloning, overexpression, purification, and characterization of the carboxyl-terminal nucleotide binding domain of P-glycoprotein.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

A study on the properties of commercial thrombin preparations

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

The role of plasmin in kinin-release by preparations of human thrombin

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Identification and isolation of vitamin K-dependent proteins by HPLC

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Structural basis for the substrate specificity of tobacco etch virus protease

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

The P1' specificity of tobacco etch virus protease

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

A novel method to determine the topology of peroxisomal membrane proteins in vivo using the tobacco etch virus protease

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Large-scale purification of a stable form of recombinant tobacco etch virus protease

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

System for cleavable Fc fusion proteins using tobacco etch virus (TEV) protease

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression, purification, refolding, and characterization of recombinant human interleukin-13: utilization of intracellular processing

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression, purification and characterization of the structure and disulfide linkages of insulin-like growth factor binding protein-4.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Inhibition of tobacco etch virus protease activity by detergents

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression of a kallikrein-like protease from the snake venom: engineering of autocatalytic site in the fusion protein to facilitate protein refolding.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-affinity technology.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression of Giardia duodenalis beta-tubulin as a soluble protein in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Overexpression and characterization of the chromosomal aminoglycoside 2'-N-acetyltransferase of Providencia stuartii

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Rat mast cell protease 4 is a beta-chymase with unusually stringent substrate recognition profile

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Construction, non-denaturing affinity purification, and characterization of baculovirally expressed human secretory leukocyte protease inhibitor

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

GFP-visualized immobilized enzymes: degradation of paraoxon via organophosphorus hydrolase in a packed column

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Integrated bioprocessing in Saccharomyces cerevisiae using green fluorescent protein as a fusion partner

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Recombinant fusion proteins for the industrial production of disulfide bridge containing peptides: purification, oxidation without concatamer formation, and selective cleavage

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

A fusion protein system for the recombinant production of short disulfide-containing peptides.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Activation of cholera toxin by ADP-ribosylation factors: 20-kDa guanine nucleotide-binding proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Examining the efficiency of receptor/G-protein coupling with a cleavable beta2-adrenoceptor-gsalpha fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression, purification and immunological characterization of the transforming protein E7, from cervical cancer-associated human papillomavirus type 16.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

A scintillation proximity assay for the Raf/MEK/ERK kinase cascade: high-throughput screening and identification of selective enzyme inhibitors

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Detection of various epitopes of murine osteopontin by monoclonal antibodies

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression, Purification, and in Vitro Characterization of the Human Outer Mitochondrial Membrane Receptor Human Translocase of the Outer Mitochondrial Membrane 20

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Cystic fibrosis transmembrane conductance regulator: the purified NBF1+R protein interacts with the purified NBF2 domain to form a stable NBF1+R/NBF2 complex while inducing a conformational change transmitted to the C-terminal region

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Interaction of voltage-gated sodium channels with the extracellular matrix molecules tenascin-C and tenascin-R

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Immunoadhesins of interleukin-6 and the IL-6/soluble IL-6R fusion protein hyper-IL-6

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Identification of quinone-binding and heme-ligating residues of the smallest membrane-anchoring subunit (QPs3) of bovine heart mitochondrial succinate:ubiquinone reductase.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression and characterisation of the heavy chain of tetanus toxin: reconstitution of the fully-recombinant dichain protein in active form

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Function of the propeptide region in recombinant expression of active procathepsin L in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Identification of a novel protein antigen encoded by a Mycobacterium tuberculosis-specific RD1 region gene.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Production of recombinant rat proopiomelanocortin1-74 and characterization of its mitogenic action on pituitary lactotrophs.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Carboxyl-terminal domain of human apolipoprotein E: expression, purification, and crystallization.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

The CDC42-specific inhibitor derived from ACK-1 blocks v-Ha-Ras-induced transformation

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Cloning, expression and immunological reactivity of two mammalian cell entry proteins encoded by the mce1 operon of Mycobacterium tuberculosis

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

The prion domain of yeast Ure2p induces autocatalytic formation of amyloid fibers by a recombinant fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

S-decyl-glutathione nonspecifically stimulates the ATPase activity of the nucleotide-binding domains of the human multidrug resistance-associated protein, MRP1 (ABCC1).

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Inhibition of poly(ADP-ribose) polymerase activity by Bcl-2 in association with the ribosomal protein S3a

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Characterization of a functional thrombin receptor. Issues and opportunities

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

IgE binding of the recombinant allergen soybean profilin (rGly m 3) is mediated by conformational epitopes

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Factor X fusion proteins: improved production and use in the release in vitro of biologically active hirudin from an inactive alpha-factor-hirudin fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Comparison of the D-glutamate-adding enzymes from selected gram-positive and gram-negative bacteria

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Construction and functional evaluation of a single-chain antibody fusion protein with fibrin targeting and thrombin inhibition after activation by factor Xa

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Kinetic and structural characterization of a two-domain streptokinase: dissection of domain functionality

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Mass spectrometric determination of a novel modification of the N-terminus of histidine-tagged proteins expressed in bacteria

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Recombinant p42IP4, a brain-specific 42-kDa high-affinity Ins(1,3,4,5)P4 receptor protein, specifically interacts with lipid membranes containing Ptd-Ins(3,4,5)P3.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Homologous plasminogen N-terminal and plasminogen-related gene A and B peptides. Characterization of cDNAs and recombinant fusion proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Production and activation of recombinant hK2 with propeptide mutations resulting in high expression levels

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Cloning, expression and characterization of the major latex allergen prohevein.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

In vivo and in vitro complementation of the N-terminal domain of enzyme I of the Escherichia coli phosphotransferase system by the cloned C-terminal domain

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

NH2-terminal structural motifs in staphylokinase required for plasminogen activation

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Immunological characterization of two major secreted forms of recombinant hepatitis B virus e antigens

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression of human tyrosine hydroxylase type I in Escherichia coli as a protease-cleavable fusion protein. Short communication

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Expression and characterisation of Plasmodium falciparum acidic basic repeat antigen expressed in Escherichia coli.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

In vitro assembly of yeast 5S rRNA and a fusion protein containing ribosomal protein L5 and maltose binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Purification and functional reconstitution of a truncated human Na(+)/glucose cotransporter (SGLT1) expressed in E. coli.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Polymerization of bacteriophage phi 29 replication protein p1 into protofilament sheets

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Hypoderma lineatum: expression of enzymatically active hypodermin C in Escherichia coli and its use for the immunodiagnosis of hypodermosis.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Functional characterization of a recombinant form of the C-terminal, globular head region of the B-chain of human serum complement protein, C1q.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Purification, characterization and biosynthesis of parabutoxin 3, a component of Parabuthus transvaalicus venom

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Excretion of human beta-endorphin into culture medium by using outer membrane protein F as a fusion partner in recombinant Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

Production of extracellular domain of human tissue factor using maltose-binding protein fusion system.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2803%2900168-2

7 January 2021

10.1016/S1046-5928(03)00168-2

Identifiers

DOI

1 reference

31 July 2017

1 reference

PubMed ID

12963335