(Q34290912)

1 August 2017

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title

Traveling for the glycosphingolipid path (English)

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1 August 2017

Hakomori S

1

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1 August 2017

publication date

1 July 2000

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1 August 2017

published in

Glycoconjugate Journal

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1 August 2017

volume

17

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1 August 2017

issue

7-9

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1 August 2017

page(s)

627-647

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Stage-specific embryonic antigen involves αl→ 3 fucosylated type 2 blood group chains

1 August 2017

cites work

1 reference

12 December 2020

Novel blood group H glycolipid antigens exclusively expressed in blood group A and AB erythrocytes (type 3 chain H). II. Differential conversion of different H substrates by A1 and A2 enzymes, and type 3 chain H expression in relation to secretor st

1 reference

12 December 2020

Tumor-Associated Carbohydrate Antigens Defining Tumor Malignancy: Basis for Development of Anti-Cancer Vaccines

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12 December 2020

Identification of a peptide which binds to the carbohydrate-specific monoclonal antibody B3

1 reference

12 December 2020

A novel glycosulfopeptide binds to P-selectin and inhibits leukocyte adhesion to P-selectin

1 reference

12 December 2020

The area-code hypothesis: the immune system provides clues to understanding the genetic and molecular basis of cell recognition during development

1 reference

12 December 2020

Further studies on cell adhesion based on Le(x)-Le(x) interaction, with new approaches: embryoglycan aggregation of F9 teratocarcinoma cells, and adhesion of various tumour cells based on Le(x) expression

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12 December 2020

[Construction of helical structure by lipids and related compounds]

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12 December 2020

Characterization of sialosylated Lewisx as a new tumor-associated antigen.

1 reference

12 December 2020

The increased level of beta1,4-galactosyltransferase required for lactose biosynthesis is achieved in part by translational control

1 reference

12 December 2020

Extended type 1 chain glycosphingolipids: dimeric Lea (III4V4Fuc2Lc6) as human tumor-associated antigen

1 reference

12 December 2020

Carbohydrate antigen profiles of human erythroleukemia cell lines HEL and K562

1 reference

12 December 2020

New aspects of siglec binding specificities, including the significance of fucosylation and of the sialyl-Tn epitope. Sialic acid-binding immunoglobulin superfamily lectins

1 reference

12 December 2020

A series of disialogangliosides with binary 2----3 sialosyllactosamine structure, defined by monoclonal antibody NUH2, are oncodevelopmentally regulated antigens

1 reference

12 December 2020

Anomeric Structures of Globoside and Ceramide Trihexoside of Human Erythrocytes and Hamster Fibroblasts

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12 December 2020

Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism

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12 December 2020

Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells.

1 reference

12 December 2020

The caveolae membrane system

1 reference

12 December 2020

Human tumor-associated Le(a)-Le(x) hybrid carbohydrate antigen IV3(Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc)III3FucnLc4 defined by monoclonal antibody 43-9F: enzymatic synthesis, structural characterization, and comparative reactivity with various anti

1 reference

12 December 2020

Structures of branched blood group A-active glycosphingolipids in human erythrocytes and polymorphism of A- and H-glycolipids in A1 and A2 subgroups

1 reference

12 December 2020

Glycosphingolipid receptor function is modified by fatty acid content. Verotoxin 1 and verotoxin 2c preferentially recognize different globotriaosyl ceramide fatty acid homologues

1 reference

12 December 2020

The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: A preliminary note

1 reference

12 December 2020

Human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum

1 reference

12 December 2020

Possible role of ceramide in defining structure and function of membrane glycolipids

1 reference

12 December 2020

Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A

1 reference

12 December 2020

Chemical synthesis of alpha-L-fucopyranosylceramide and its analogues and preparation of antibodies directed to this glycolipid

1 reference

12 December 2020

Disialosyl galactosylgloboside as an adhesion molecule expressed on renal cell carcinoma and its relationship to metastatic potential

1 reference

12 December 2020

Regulation of the GnT-V promoter by transcription factor Ets-1 in various cancer cell lines

1 reference

12 December 2020

Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells

1 reference

12 December 2020

Separation of "glycosphingolipid signaling domain" from caveolin-containing membrane fraction in mouse melanoma B16 cells and its role in cell adhesion coupled with signaling

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12 December 2020

A ceramide tetrasaccharide of human erythrocyte membrane reacting with anti-type XIV pneumococcal polysaccharide antiserum

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12 December 2020

Structures of ceramide tetrasaccharides from various sources: uniqueness of rat kidney ceramide tetrasaccharide

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12 December 2020

Motility inhibition and apoptosis are induced by metastasis-suppressing gene product CD82 and its analogue CD9, with concurrent glycosylation

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12 December 2020

ELAM-1 Mediates Cell Adhesion by Recognition of a Carbohydrate Ligand, Sialyl-Le x

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12 December 2020

Immunochemical reactions of lipids

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12 December 2020

Protective effects of oligosaccharides in P-selectin-dependent lung injury

1 reference

12 December 2020

Novel fucolipids of human adenocarcinoma: characterization of the major Ley antigen of human adenocarcinoma as trifucosylnonaosyl Ley glycolipid (III3FucV3FucVI2FucnLc6)

1 reference

12 December 2020

The globoseries glycosphingolipid sialosyl galactosyl globoside is found in urinary tract tissues and is a preferred binding receptor In vitro for uropathogenic Escherichia coli expressing pap-encoded adhesins.

1 reference

12 December 2020

Sialosyl-fucosyl Poly-LacNAc without the sialosyl-Lex epitope as the physiological myeloid cell ligand in E-selectin-dependent adhesion: studies under static and dynamic flow conditions

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12 December 2020

Common tetrasaccharide epitope NeuAc alpha 2-->3Gal beta 1-->3(Neu-Ac alpha 2-->6)GalNAc, presented by different carrier glycosylceramides or O-linked peptides, is recognized by different antibodies and ligands having distinct specificities

1 reference

12 December 2020

Monogamous bivalency of IgG antibodies. I. Deficiency of branched ABHI-active oligosaccharide chains on red cells of infants causes the weak antiglobulin reactions in hemolytic disease of the newborn due to ABO incompatibility

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12 December 2020

Blood group A determinants with mono- and difucosyl type 1 chain in human erythrocyte membranes

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12 December 2020

A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen SSEA-1

1 reference

12 December 2020

Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo

1 reference

12 December 2020

A fully synthetic immunogen carrying a carcinoma-associated carbohydrate for active specific immunotherapy

1 reference

12 December 2020

Deletion of A-antigen in a human cancer cell line is associated with reduced promoter activity of CBF/NF-Y binding region, and possibly with enhanced DNA methylation of A transferase promoter

1 reference

12 December 2020

Three types of blood group I specificity among monoclonal anti-I autoantibodies revealed by analogues of a branched erythrocyte glycolipid

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12 December 2020

A carbohydrate epitope associated with human squamous lung cancer.

1 reference

12 December 2020

Localization of epidermal growth factor-stimulated Ras/Raf-1 interaction to caveolae membrane

1 reference

12 December 2020

Peptide mimicking sialyl-Lewis(a) with anti-inflammatory activity

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12 December 2020

Ganglioside-mediated modulation of cell growth, growth factor binding, and receptor phosphorylation

1 reference

12 December 2020

Glycoconjugate expression during embryogenesis and its biological significance

1 reference

12 December 2020

Lacto-N-neotetraosylceramide (“paragloboside”) as a possible tumor) associated surface antigen of hamster NILpy tumor

1 reference

12 December 2020

Siglecs: a family of sialic-acid binding lectins

1 reference

12 December 2020

A Close Association of GM3 with c-Src and Rho in GM3-Enriched Microdomains at the B16 Melanoma Cell Surface Membrane: A Preliminary Note

1 reference

12 December 2020

Antibody affinity. VII. Multivalent interaction of anti-lactoside antibody.

1 reference

12 December 2020

New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3

1 reference

12 December 2020

Ganglioside-mediated modulation of cell growth. Specific effects of GM3 on tyrosine phosphorylation of the epidermal growth factor receptor

1 reference

12 December 2020

Membrane differentiation in human erythroid cells: unique profiles of cell surface glycoproteins expressed in erythroblasts in vitro from three ontogenic stages

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12 December 2020

An Arg-Gly-Asp-directed receptor on the surface of human melanoma cells exists in an divalent cation-dependent functional complex with the disialoganglioside GD2.

1 reference

12 December 2020

Lea-active heptaglycosylceramide, a hybrid of type 1 and type 2 chain, and the pattern of glycolipids with Lea, Leb, X (Lex), and Y (Ley) determinants in human blood cell membranes (ghosts). Evidence that type 2 chain can elongate repetitively but t

1 reference

12 December 2020

A novel disialoganglioside (IV3NeuAcIII6NeuAcLc4) of human adenocarcinoma and the monoclonal antibody (FH9) defining this disialosyl structure

1 reference

12 December 2020

Further characterization of type 2 and type 3 chain blood group A glycosphingolipids from human erythrocyte membranes

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12 December 2020

Mice with disrupted GM2/GD2 synthase gene lack complex gangliosides but exhibit only subtle defects in their nervous system

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12 December 2020

On neutral fucoglycolipids having long, branched carbohydrate chains: H-active and I-active glycosphingolipids of human erythrocyte membranes

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12 December 2020

Further studies on sperm-immobilizing antibody found in sera of unexplained cases of sterility in women

1 reference

12 December 2020

Antibody affinity—III the role of multivalence

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12 December 2020

The tetraspanin superfamily: molecular facilitators

1 reference

12 December 2020

Novel fucolipids accumulating in human adenocarcinoma. III. A hybridoma antibody (FH6) defining a human cancer-associated difucoganglioside (VI3NeuAcV3III3Fuc2nLc6)

1 reference

12 December 2020

Vaccination with carbohydrate peptide mimotopes promotes anti-tumor responses.

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12 December 2020

Synthetic carbohydrate vaccines: synthesis and immunogenicity of Tn antigen conjugates.

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12 December 2020

The design and synthesis of mimetics of peptide beta-turns

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12 December 2020

Lipid modulation of glycolipid receptor function. Availability of Gal(alpha 1-4)Gal disaccharide for verotoxin binding in natural and synthetic glycolipids

1 reference

12 December 2020

Surface carbohydrates of hamster fibroblasts. I. Chemical characterization of surface-labeled glycosphingolipids and aspecific ceramide tetrasaccharide for transformants

1 reference

12 December 2020

Association of Src family tyrosine kinase Lyn with ganglioside GD3 in rat brain. Possible regulation of Lyn by glycosphingolipid in caveolae-like domains.

1 reference

12 December 2020

GM3 ganglioside induces hamster fibrobiast growth inhibition in chemically-defined medium: Ganglioside may regulate growth factor receptor function

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12 December 2020

Isolation, characterization, and biosynthesis of Forssman antigen in human lung and lung carcinoma

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12 December 2020

Regulatory role of GM3 ganglioside in alpha 5 beta 1 integrin receptor for fibronectin-mediated adhesion of FUA169 cells.

1 reference

12 December 2020

Effect of Synthetic Sialyl 2→1 Sphingosine and Other Glycosylsphingosines on the Structure and Function of the “Glycosphingolipid Signaling Domain (GSD)” in Mouse Melanoma B16 Cells†

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12 December 2020

Gangliosides influence angiogenesis in an experimental mouse brain tumor.

1 reference

12 December 2020

Aberrant glycosylation in tumors and tumor-associated carbohydrate antigens.

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12 December 2020

Isolation of a primate embryonic stem cell line

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12 December 2020

Inhibition of highly metastatic FBJ-LL cell migration by ganglioside GD1a highly expressed in poorly metastatic FBJ-S1 cells

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12 December 2020

Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells

1 reference

12 December 2020

Blood group i and I activities of "lacto-N-norhexaosylceramide" and its analogues: the structural requirements for i-specificities

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12 December 2020

Structural studies of lipid fibers formed by sphingosine

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12 December 2020

The alpha 1-->3 fucosylation at the penultimate GlcNAc catalyzed by fucosyltransferase VII is blocked by internally fucosylated residue in sialosyl long-chain poly-LacNAc: enzymatic basis for expression of physiological E-selectin epitope

1 reference

12 December 2020

Accumulation of glucosylceramides in multidrug-resistant cancer cells

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12 December 2020

Mice lacking complex gangliosides develop Wallerian degeneration and myelination defects

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12 December 2020

Novel fucolipids of human adenocarcinoma: monoclonal antibody specific for trifucosyl Ley (III3FucV3FucVI2FucnLc6) and a possible three-dimensional epitope structure

1 reference

12 December 2020

Factors affecting expression of glycolipid tumor antigens: influence of ceramide composition and coexisting glycolipid on the antigenicity of gangliotriaosylceramide in murine lymphoma cells

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12 December 2020

Carbohydrate-mediated sorting in aggregating embryonal carcinoma cells

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12 December 2020

Cellular blood group substances. III. Isolation and characterization of glycosphingolipids with blood group H specificity from membranes of human erythrocytes

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12 December 2020

Localization of platelet-derived growth factor-stimulated phosphorylation cascade to caveolae

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12 December 2020

Globoside-dependent adhesion of human embryonal carcinoma cells, based on carbohydrate-carbohydrate interaction, initiates signal transduction and induces enhanced activity of transcription factors AP1 and CREB

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12 December 2020

Characterization of a blood group I-active ganglioside. Structural requirements for I and i specificities

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12 December 2020

A Sphingolipid Having a Novel Type of Ceramide and Lacto-N-Fucopentaose III

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12 December 2020

New insights in glycosphingolipid function: "glycosignaling domain," a cell surface assembly of glycosphingolipids with signal transducer molecules,involved in cell adhesion coupled with signaling.

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12 December 2020

Selective inhibition of cell growth and associated changes in glycolipid metabolism induced by monovalent antibodies to glycolipids

1 reference

12 December 2020

Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: a phase I trial in patients with malignant melanoma

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12 December 2020

A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody

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12 December 2020

E-cadherin null mutant embryos fail to form a trophectoderm epithelium

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12 December 2020

Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability.

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12 December 2020

Cancer-associated glycosphingolipid antigens: their structure, organization, and function

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12 December 2020

New directions in cancer therapy based on aberrant expression of glycosphingolipids: anti-adhesion and ortho-signaling therapy

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12 December 2020

Synergistic effect of two cell recognition systems: glycosphingolipid-glycosphingolipid interaction and integrin receptor interaction with pericellular matrix protein

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12 December 2020

The isolation and some properties of the Forssman hapten from sheep erythrocytes

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12 December 2020

Design and synthesis of a mimetic from an antibody complementarity-determining region.

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12 December 2020

Differentiation of a cell line of myeloid leukemia

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12 December 2020

Uncoupling ceramide glycosylation by transfection of glucosylceramide synthase antisense reverses adriamycin resistance

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12 December 2020

Cell adhesion in a dynamic flow system as compared to static system. Glycosphingolipid-glycosphingolipid interaction in the dynamic system predominates over lectin- or integrin-based mechanisms in adhesion of B16 melanoma cells to non-activated endo

1 reference

12 December 2020

Human lung adenocarcinoma cell lines with different lung colonization potential (LCP), and a correlation between expression of sialosyl dimeric Lex (defined by MAb FH6) and LCP

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12 December 2020

Novel Endothelial Cell Activation Factor(s) Released from Activated Platelets Which Induce E-Selectin Expression and Tumor Cell Adhesion to Endothelial Cells: A Preliminary Note

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12 December 2020

Inhibition of b16 melanoma metastasis by administration of g(m3)- or gg3- liposomes - blocking adhesion of melanoma-cells to endothelial-cells (antiadhesion therapy) via inhibition of g(m3)-gg3cer or g(m3)-laccer interaction

1 reference

12 December 2020

GD1alpha-replica peptides functionally mimic GD1alpha, an adhesion molecule of metastatic tumor cells, and suppress the tumor metastasis

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12 December 2020

A carbohydrate domain common to both sialyl Le(a) and sialyl Le(X) is recognized by the endothelial cell leukocyte adhesion molecule ELAM-1

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12 December 2020

Isoantigenic expression of Forssman glycolipid in human gastric and colonic mucosa: Its possible identity with “A-like antigen” in human cancer

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12 December 2020

Male-specific modification of human CD52

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12 December 2020

Antigen structure and genetic basis of histo-blood groups A, B and O: their changes associated with human cancer

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12 December 2020

Suppression of ganglioside GD3 expression in a rat F-11 tumor cell line reduces tumor growth, angiogenesis, and vascular endothelial growth factor production.

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12 December 2020

Binding of uropathogenic Escherichia coli R45 to glycolipids extracted from vaginal epithelial cells is dependent on histo-blood group secretor status

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12 December 2020

Developmentally regulated expression of cell surface carbohydrates during mouse embryogenesis

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12 December 2020

Sterility in female guinea pigs induced by injection with testis

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12 December 2020

Gal-Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli

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12 December 2020

Anti-sperm antibodies from infertile patients and their cognate sperm antigens: a review. Identity between SAGA-1, the H6-3C4 antigen, and CD52.

1 reference

12 December 2020

Induction of alpha-N-acetylgalactosamine-O-serine/threonine (Tn) antigen-mediated cellular immune response for active immunotherapy in mice

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12 December 2020

Transcription of human ABO histo-blood group genes is dependent upon binding of transcription factor CBF/NF-Y to minisatellite sequence

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12 December 2020

Molecular arrangements in sphingolipids. Conformation and hydrogen bonding of ceramide and their implication on membrane stability and permeability

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12 December 2020

In vitro and in vivo antitumor effects of murine monoclonal antibody NCC-ST-421 reacting with dimeric Le(a) (Le(a)/Le(a)) epitope

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12 December 2020

Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium

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12 December 2020

Glycopeptides bind MHC molecules and elicit specific T cell responses

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12 December 2020

Functional breakdown of the lipid bilayer of the myelin membrane in central and peripheral nervous system by disrupted galactocerebroside synthesis

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12 December 2020

Anti-i: a cold antibody defining the Ii relationship in human red cells

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12 December 2020

Specific interaction between gangliotriaosylceramide (Gg3) and sialosyllactosylceramide (GM3) as a basis for specific cellular recognition between lymphoma and melanoma cells

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12 December 2020

Status of blood group carbohydrate chains in ontogenesis and in oncogenesis

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12 December 2020

Potent immunogenic short linear peptide constructs composed of B cell epitopes and Pan DR T helper epitopes (PADRE) for antibody responses in vivo

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12 December 2020

Enhancing the effect of THERATOPE STn-KLH cancer vaccine in patients with metastatic breast cancer by pretreatment with low-dose intravenous cyclophosphamide

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12 December 2020

Characterization of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neoplastic epithelial cells of human mammary gland

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12 December 2020

Association of the glycolipid pattern with antigenic alterations in mouse fibroblasts transformed by murine sarcoma virus

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12 December 2020

Improved survival in stage III melanoma patients with GM2 antibodies: a randomized trial of adjuvant vaccination with GM2 ganglioside

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12 December 2020

Galectin-1 is a major receptor for ganglioside GM1, a product of the growth-controlling activity of a cell surface ganglioside sialidase, on human neuroblastoma cells in culture

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12 December 2020

AGGLUTINABILITY OF RED CELLS BY ANTI-I IN PATIENTS WITH THALASSEMIA MAJOR AND OTHER HAEMATOLOGICAL DISORDERS.

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12 December 2020

Reconstitution of membranes simulating "glycosignaling domain" and their susceptibility to lyso-GM3

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12 December 2020

Protective effects of sialylated oligosaccharides in immune complex-induced acute lung injury

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12 December 2020

A revised structure for the Forssman glycolipid hapten

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12 December 2020

Characterization of anti-Forssman (anti-Fs) antibodies in human sera: their specificity and possible changes in patients with cancer

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12 December 2020

Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1)

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12 December 2020

Extended type-1 chain glycosphingolipid antigens. Isolation and characterization of trifucosyl-Leb antigen (III4V4VI2Fuc3Lc6).

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12 December 2020

Induction of anti-tumor cytotoxic T lymphocytes in normal humans using primary cultures and synthetic peptide epitopes

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12 December 2020

Regulation of epidermal growth factor receptor signal transduction. Role of gangliosides

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12 December 2020

A multivalent lacto-N-fucopentaose III-lysyllysine conjugate decompacts preimplantation mouse embryos, while the free oligosaccharide is ineffective

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12 December 2020

Antitumor activity via inhibition of glycosphingolipid biosynthesis.

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12 December 2020

A vital role for glycosphingolipid synthesis during development and differentiation

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12 December 2020

Ganglioside GD1alpha functions in the adhesion of metastatic tumor cells to endothelial cells of the target tissue.

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12 December 2020

Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 2. Characterization of E-selectin binding fractions, and structural requirements for physiological binding to E-selectin

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12 December 2020

Cancer antigens: how specific should they be?

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12 December 2020

A-B Hemolytic disease of the newborn; analysis of 1480 cord blood specimens, with special reference to the direct antiglobulin test and to the group O mother

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12 December 2020

A reflection on the early history of glycosphingolipids

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12 December 2020

Dual roles of sialyl Lewis X oligosaccharides in tumor metastasis and rejection by natural killer cells

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12 December 2020

Cell adhesion, spreading, and motility of GM3-expressing cells based on glycolipid-glycolipid interaction

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12 December 2020

Specificity and immunobiological properties of monoclonal antibody IMH2, established after immunization with Le(b)/Le(a) glycosphingolipid, a novel extended type 1 chain antigen

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12 December 2020

Sequential change of carbohydrate antigen associated with differentiation of murine leukemia cells: i-I antigenic conversion and shifting of glycolipid synthesis.

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12 December 2020

MHC-restricted, glycopeptide-specific T cells show specificity for both carbohydrate and peptide residues.

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12 December 2020

A peptide mimic of E-selectin ligand inhibits sialyl Lewis X-dependent lung colonization of tumor cells

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12 December 2020

Human alpha (1,3)-fucosyltransferase IV (FUTIV) gene expression is regulated by elk-1 in the U937 cell line

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12 December 2020

Mutants of embryonal carcinoma cells defective in the expression of embryoglycan

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12 December 2020

Rapid induction of cytokine and E-selectin expression in the liver in response to metastatic tumor cells.

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12 December 2020

Glycolipid core structure switching from globo- to lacto- and ganglio-series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells

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12 December 2020

Le(y) antigen expression is correlated with apoptosis (programmed cell death)

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12 December 2020

alpha-L-Fucopyranosylceramide, a novel glycolipid accumulated in some of the human colon tumors

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12 December 2020

Novel fucolipids accumulating in human adenocarcinoma. I. Glycolipids with di- or trifucosylated type 2 chain

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12 December 2020

Carbohydrate vaccines in cancer: immunogenicity of a fully synthetic globo H hexasaccharide conjugate in man.

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12 December 2020

Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemia

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12 December 2020

Ganglioside inhibition of fibronectin-mediated cell adhesion to collagen

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12 December 2020

Establishment and characterization of a human hybridoma secreting monoclonal antibody with high titers of sperm immobilizing and agglutinating activities against human seminal plasma.

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12 December 2020

Identifiers

DOI

10.1023/A:1011086929064

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Dimensions Publication ID

1 August 2017

1039515530

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