(Q34369163)

1 August 2017

title

Assembly, purification and crystallization of an active HIV-1 reverse transcriptase initiation complex (English)

1 reference

1 August 2017

Janice D Pata

1

1 reference

1 August 2017

Bradford R King

series ordinal

2

1 reference

1 August 2017

Thomas A Steitz

series ordinal

3

1 reference

1 August 2017

language of work or name

English

0 references

publication date

1 November 2002

1 reference

1 August 2017

published in

Nucleic Acids Research

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1 August 2017

volume

30

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1 August 2017

issue

22

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1 August 2017

page(s)

4855-4863

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Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA

1 August 2017

cites work

1 reference

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NMR and biochemical characterization of recombinant human tRNA(Lys)3 expressed in Escherichia coli: identification of posttranscriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription

6 July 2018

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The crystal structure of HIV reverse-transcription primer tRNA(Lys,3) shows a canonical anticodon loop

6 July 2018

1 reference

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Inhibition of the initiation of HIV-1 reverse transcription by 3'-azido-3'-deoxythymidine. Comparison with elongation

6 July 2018

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High-performance liquid chromatography purification of homogenous-length RNA produced by trans cleavage with a hammerhead ribozyme

6 July 2018

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Role of post-transcriptional modifications of primer tRNALys,3 in the fidelity and efficacy of plus strand DNA transfer during HIV-1 reverse transcription

6 July 2018

1 reference

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Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution

6 July 2018

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Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance

6 July 2018

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Pre-steady-state kinetic characterization of RNA-primed initiation of transcription by HIV-1 reverse transcriptase and analysis of the transition to a processive DNA-primed polymerization mode

6 July 2018

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Implication of the tRNA initiation step for human immunodeficiency virus type 1 reverse transcriptase in the mechanism of 3'-azido-3'-deoxythymidine (AZT) resistance

6 July 2018

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The structure of HIV-1 reverse transcriptase complexed with an RNA pseudoknot inhibitor

6 July 2018

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Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme

6 July 2018

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Mutating a region of HIV-1 reverse transcriptase implicated in tRNA(Lys-3) binding and the consequences for (-)-strand DNA synthesis

6 July 2018

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Interaction of retroviral reverse transcriptase with template-primer duplexes during replication.

6 July 2018

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Binding and kinetic properties of HIV-1 reverse transcriptase markedly differ during initiation and elongation of reverse transcription.

6 July 2018

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Preparative-scale purification of RNA using an efficient method which combines gel electrophoresis and column chromatography

6 July 2018

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Human immunodeficiency virus type 1 reverse transcriptase and early events in reverse transcription

6 July 2018

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Initiation of (-) strand DNA synthesis from tRNA(3Lys) on lentiviral RNAs: implications of specific HIV-1 RNA-tRNA(3Lys) interactions inhibiting primer utilization by retroviral reverse transcriptases.

6 July 2018

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Specific initiation and switch to elongation of human immunodeficiency virus type 1 reverse transcription require the post-transcriptional modifications of primer tRNA3Lys

6 July 2018

1 reference

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Kinetic analysis of intravirion reverse transcription in the blood plasma of human immunodeficiency virus type 1-infected individuals: direct assessment of resistance to reverse transcriptase inhibitors in vivo

6 July 2018

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Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA

6 July 2018

1 reference

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Site-specific crosslinking of 4-thiouridine-modified human tRNA(3Lys) to reverse transcriptase from human immunodeficiency virus type I.

6 July 2018

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Human immunodeficiency virus-1 reverse transcriptase heterodimer stability

6 July 2018

1 reference

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Subunit specificity of mutations that confer resistance to nonnucleoside inhibitors in human immunodeficiency virus type 1 reverse transcriptase

6 July 2018

1 reference

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Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

6 July 2018

1 reference

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A simple procedure for maximum yield of high-quality plasmid DNA.

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=137168

Characterization of self-cleavage of viroid and virusoid RNAs

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=137168

Interaction of HIV-1 reverse transcriptase with a synthetic form of its replication primer, tRNALys,3

6 July 2018

1 reference

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Factors underlying spontaneous inactivation and susceptibility to neutralization of human immunodeficiency virus

6 July 2018

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Reverse transcriptase of human immunodeficiency virus can use either human tRNA(3Lys) or Escherichia coli tRNA(2Gln) as a primer in an in vitro primer-utilization assay

6 July 2018

1 reference

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Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor

6 July 2018

1 reference

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Initiation of minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase

6 July 2018

1 reference

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Purification and characterization of human immunodeficiency virus type 1 reverse transcriptase

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=137168

Crystallization of RNA-protein complexes. I. Methods for the large-scale preparation of RNA suitable for crystallographic studies

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=137168

Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography.

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=137168

Primer Selection by HIV-1 Reverse Transcriptase on RNA – tRNA3Lysand DNA – tRNA3LysHybrids

27 October 2018

1 reference

12 December 2020

HIV-1 reverse transcriptase discriminates against non-self tRNA primers

1 reference

12 December 2020

Establishing suitability of RNA preparations for crystallization. Determination of polydispersity

1 reference

12 December 2020

Contacts between Reverse Transcriptase and the Primer Strand Govern the Transition from Initiation to Elongation of HIV-1 Reverse Transcription

1 reference

12 December 2020

High-resolution preparative-scale purification of RNA using the Prep Cell

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/GKF620

1 reference

1 August 2017

1 reference

1 August 2017

1 reference