(Q34903967)

5 August 2017

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title

Recent advances in the application of expressed protein ligation to protein engineering (English)

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5 August 2017

Roseanne M Hofmann

1

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5 August 2017

Tom W Muir

series ordinal

2

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5 August 2017

publication date

1 August 2002

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Current Opinion in Biotechnology

5 August 2017

published in

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5 August 2017

volume

13

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5 August 2017

issue

4

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5 August 2017

page(s)

297-303

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Protein splicing and its applications

5 August 2017

cites work

1 reference

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7 January 2021

Synthesis of proteins by native chemical ligation

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7 January 2021

Synthesis of native proteins by chemical ligation

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7 January 2021

Expressed protein ligation: a general method for protein engineering

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7 January 2021

Expressed protein ligation, a novel method for studying protein-protein interactions in transcription

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7 January 2021

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

1 reference

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7 January 2021

Semisynthesis of cytotoxic proteins using a modified protein splicing element

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7 January 2021

Covalent capture: a new tool for the purification of synthetic and recombinant polypeptides

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7 January 2021

Backbone Amide Linker (BAL) Strategy forNα-9-Fluorenylmethoxycarbonyl (Fmoc) Solid-Phase Synthesis of Unprotected Peptidep-Nitroanilides and Thioesters1

1 reference

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7 January 2021

Solid Phase Synthesis of Peptide C-Terminal Thioesters by Fmoc/t-Bu Chemistry

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7 January 2021

Improved synthesis of C-terminal peptide thioesters on "safety-catch" resins using LiBr/THF

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7 January 2021

Facile, fmoc-compatible solid-phase synthesis of peptide C-terminal thioesters

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https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Fmoc-Compatible Solid-Phase Peptide Synthesis of Long C-Terminal Peptide Thioesters The authors are grateful to the ETH-Zürich and Novartis Pharma AG for support of this work. Fmoc=9-fluorenylmethoxycarbonyl

1 reference

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7 January 2021

Efficient semisynthesis of a tetraphosphorylated analogue of the Type I TGFbeta receptor

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https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Engineering an unnatural Nalpha-anchored disulfide into BPTI by total chemical synthesis: structural and functional consequences

1 reference

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7 January 2021

Total synthesis of cytochrome b562 by native chemical ligation using a removable auxiliary

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7 January 2021

A new scaffold for amide ligation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Synthesis of peptides and proteins without cysteine residues by native chemical ligation combined with desulfurization

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https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Synthesis of a selenocysteine-containing peptide by native chemical ligation

1 reference

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7 January 2021

Selenocysteine in native chemical ligation and expressed protein ligation.

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7 January 2021

Convergent synthesis of peptide conjugates using dehydroalanines for chemoselective ligations

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7 January 2021

The TGF beta receptor activation process: an inhibitor- to substrate-binding switch

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7 January 2021

Crystal Structure of a Phosphorylated Smad2

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7 January 2021

Protein tyrosine kinase Csk-catalyzed phosphorylation of Src containing unnatural tyrosine analogues

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Fluorescent monitoring of kinase activity in real time: development of a robust fluorescence-based assay for Abl tyrosine kinase activity

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

High-Yielding Staudinger Ligation of a Phosphinothioester and Azide To Form a Peptide

1 reference

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7 January 2021

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation

1 reference

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7 January 2021

Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Protein transduction: unrestricted delivery into all cells?

1 reference

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7 January 2021

A peptide carrier for the delivery of biologically active proteins into mammalian cells.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

The orientation of the AP-1 heterodimer on DNA strongly affects transcriptional potency.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

A method for the amidation of recombinant peptides expressed as intein fusion proteins in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Rescuing a destabilized protein fold through backbone cyclization

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Hedgehog patterning activity: role of a lipophilic modification mediated by the carboxy-terminal autoprocessing domain

1 reference

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7 January 2021

Enhanced potency of human Sonic hedgehog by hydrophobic modification.

1 reference

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7 January 2021

Semisynthesis and folding of the potassium channel KcsA

1 reference

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7 January 2021

Circular β-lactamase: stability enhancement by cyclizing the backbone

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Expressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Polypeptide synthesis using an expressed peptide as a building block for condensation with a peptide thioester: application to the synthesis of phosphorylated p21Max protein(1-101).

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Semi-synthetic Rab proteins as tools for studying intermolecular interactions

1 reference

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7 January 2021

Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA.

1 reference

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7 January 2021

Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

The C-terminal carboxy group of T7 RNA polymerase ensures efficient magnesium ion-dependent catalysis.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2802%2900326-9

7 January 2021

10.1016/S0958-1669(02)00326-9

Identifiers

DOI

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5 August 2017

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