(Q35053237)

6 August 2017

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title

High-throughput protein expression for the post-genomic era. (English)

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6 August 2017

Stephen P Chambers

1

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6 August 2017

language of work or name

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1 July 2002

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6 August 2017

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6 August 2017

volume

7

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6 August 2017

issue

14

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6 August 2017

page(s)

759-765

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The Sequence of the Human Genome

6 August 2017

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Finding function through structural genomics

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7 January 2021

Design of high-throughput methods of protein production for structural biology

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7 January 2021

A magnetic attraction to high-throughput genomics.

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7 January 2021

High-throughput expression of fusion proteins

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7 January 2021

A human cDNA library for high-throughput protein expression screening

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7 January 2021

High-throughput protein expression of cDNA products as a tool in functional genomics

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7 January 2021

High-throughput proteomics: protein expression and purification in the postgenomic world.

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Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins

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7 January 2021

Protein expression strategies for identification of novel target proteins

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7 January 2021

From genes to proteins: high-throughput expression and purification of the human proteome

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7 January 2021

Production and purification of novel secreted human proteins.

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7 January 2021

Direct expression of PCR products in a cell‐free transcription/translation system: synthesis of antibacterial peptide cecropin

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7 January 2021

A highly efficient cell-free protein synthesis system from Escherichia coli

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7 January 2021

Selenomethionine incorporation into a protein by cell-free synthesis

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7 January 2021

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A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

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7 January 2021

High-yield, in vitro protein expression using a continuous-exchange, coupled transcription/ translation system

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DNA cloning using in vitro site-specific recombination

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Production of Human Beta Interferon in Insect Cells Infected with a Baculovirus Expression Vector

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7 January 2021

Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector

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7 January 2021

Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells

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7 January 2021

Low-cost microbioreactor for high-throughput bioprocessing

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7 January 2021

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

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7 January 2021

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

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7 January 2021

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

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7 January 2021

High-Level Expression of Soluble Protein inEscherichia coliUsing a His6-Tag and Maltose-Binding-Protein Double-Affinity Fusion System

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7 January 2021

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

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7 January 2021

Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes

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7 January 2021

Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis

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7 January 2021

X-ray Structure of Bacteriorhodopsin at 2.5 Angstroms from Microcrystals Grown in Lipidic Cubic Phases

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7 January 2021

Screening for soluble expression of recombinant proteins in a 96-well format

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7 January 2021

Mutations in human interferon gamma affecting inclusion body formation identified by a general immunochemical screen

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7 January 2021

Catalytic domain of human immunodeficiency virus type 1 integrase: identification of a soluble mutant by systematic replacement of hydrophobic residues

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7 January 2021

Overexpression, Purification, and Crystallization of the DNA Binding and Dimerization Domains of the Epstein-Barr Virus Nuclear Antigen 1

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7 January 2021

Rapid protein-folding assay using green fluorescent protein

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7 January 2021

A simple in vivo assay for increased protein solubility

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7 January 2021

Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein

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7 January 2021

Gene expression response to misfolded protein as a screen for soluble recombinant protein

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7 January 2021

10.1016/S1359-6446(02)02361-9

Identifiers

DOI

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6 August 2017

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