(Q35053237)

6 August 2017

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title

High-throughput protein expression for the post-genomic era. (English)

1 reference

6 August 2017

Stephen P Chambers

1

1 reference

PubMed ID

12547032

6 August 2017

language of work or name

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1 July 2002

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6 August 2017

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6 August 2017

7

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6 August 2017

14

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6 August 2017

759-765

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The Sequence of the Human Genome

6 August 2017

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Initial sequencing and analysis of the human genome

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Basic local alignment search tool

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Finding function through structural genomics

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Design of high-throughput methods of protein production for structural biology

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

A magnetic attraction to high-throughput genomics.

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

High-throughput expression of fusion proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

A human cDNA library for high-throughput protein expression screening

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

High-throughput protein expression of cDNA products as a tool in functional genomics

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

High-throughput proteomics: protein expression and purification in the postgenomic world.

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

1 reference

7 January 2021

Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Protein expression strategies for identification of novel target proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

From genes to proteins: high-throughput expression and purification of the human proteome

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Production and purification of novel secreted human proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Direct expression of PCR products in a cell-free transcription/translation system: synthesis of antibacterial peptide cecropin

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

A highly efficient cell-free protein synthesis system from Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Selenomethionine incorporation into a protein by cell-free synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Cell-free production and stable-isotope labeling of milligram quantities of proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

1 reference

7 January 2021

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

High-yield, in vitro protein expression using a continuous-exchange, coupled transcription/ translation system

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

DNA cloning using in vitro site-specific recombination

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

FLEXGene repository: from sequenced genomes to gene repositories for high-throughput functional biology and proteomics

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Production of Human Beta Interferon in Insect Cells Infected with a Baculovirus Expression Vector

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Chromosomal integration of transduced recombinant baculovirus DNA in mammalian cells

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Low-cost microbioreactor for high-throughput bioprocessing

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phases

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Screening for soluble expression of recombinant proteins in a 96-well format

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Mutations in human interferon gamma affecting inclusion body formation identified by a general immunochemical screen

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Catalytic domain of human immunodeficiency virus type 1 integrase: identification of a soluble mutant by systematic replacement of hydrophobic residues

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Overexpression, purification, and crystallization of the DNA binding and dimerization domains of the Epstein-Barr virus nuclear antigen 1.

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Rapid protein-folding assay using green fluorescent protein

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

A simple in vivo assay for increased protein solubility

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

Gene expression response to misfolded protein as a screen for soluble recombinant protein.

1 reference

https://api.crossref.org/works/10.1016%2FS1359-6446%2802%2902361-9

7 January 2021

10.1016/S1359-6446(02)02361-9

Identifiers

DOI

1 reference

6 August 2017

1 reference

PubMed ID

12547032