(Q35311357)

7 August 2017

title

Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution (English)

1 reference

7 August 2017

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Julia H Appleby-Tagoe

1

1 reference

7 August 2017

Ilka V Thiel

2

1 reference

7 August 2017

Yi Wang

3

1 reference

7 August 2017

Yanfei Wang

4

1 reference

7 August 2017

Henning D Mootz

5

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7 August 2017

Xiang-Qin Liu

6

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7 August 2017

language of work or name

English

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publication date

8 August 2011

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Journal of Biological Chemistry

7 August 2017

published in

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7 August 2017

volume

286

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7 August 2017

issue

39

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7 August 2017

page(s)

34440-34447

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Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells

7 August 2017

cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein trans-splicing on an M13 bacteriophage: towards directed evolution of a semisynthetic split intein by phage display.

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Selection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Center

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Split inteins as versatile tools for protein semisynthesis

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Modulation of intein activity by its neighboring extein substrates

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Traceless protein splicing utilizing evolved split inteins

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein trans-splicing as a means for viral vector-mediated in vivo gene therapy

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein splicing in cis and in trans

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein ligation: an enabling technology for the biophysical analysis of proteins

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Directed evolution of ligand dependence: small-molecule-activated protein splicing

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo.

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

The crystal structure of aminoglycoside-3'-phosphotransferase-IIa, an enzyme responsible for antibiotic resistance

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Semisynthesis of proteins by expressed protein ligation

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

A genetic approach to identifying mitochondrial proteins

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein trans-splicing to produce herbicide-resistant acetolactate synthase

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein-splicing intein: Genetic mobility, origin, and evolution

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein splicing and related forms of protein autoprocessing

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Dissecting the Chemistry of Protein Splicing and Its Applications

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Production of cyclic peptides and proteins in vivo

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

The mechanism of protein splicing and its modulation by mutation

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing.

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing.

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Minimization and stabilization of the Mycobacterium tuberculosis recA intein

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Synthetic two-piece and three-piece split inteins for protein trans-splicing

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Temperature-sensitive control of protein activity by conditionally splicing inteins

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3190829

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

26 September 2018

1 reference

Crossref

https://api.crossref.org/works/10.1074%2FJBC.M111.277350

Circular β-lactamase: stability enhancement by cyclizing the backbone

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1074%2FJBC.M111.277350

Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1074%2FJBC.M111.277350

Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction

21 January 2018

1 reference

12 December 2020

Photocontrol of protein activity mediated by the cleavage reaction of a split intein

1 reference

12 December 2020

Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803

1 reference

12 December 2020

Activation of protein splicing with light in yeast

1 reference

12 December 2020

A genetic system yields self-cleaving inteins for bioseparations

1 reference

12 December 2020

Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A

1 reference

12 December 2020

Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein

1 reference

12 December 2020

Identifiers

DOI

10.1074/JBC.M111.277350

1 reference

7 August 2017

1 reference

7 August 2017

1 reference