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Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation.
scientific article published on 3 December 2015
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title
Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation
(English)
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superresolution
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Dirk Görlich
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10
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Tino Pleiner
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1
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Mark Bates
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2
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Sergei Trakhanov
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3
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Jan Erik Schliep
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5
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1 March 2020
Hema Chug
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6
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Marc Böhning
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7
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Henning Urlaub
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9
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Chung-Tien Lee
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4
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1 March 2020
Holger Stark
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8
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publication date
3 December 2015
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1 March 2020
published in
eLife
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4
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e11349
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1 March 2020
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Selection and identification of single domain antibody fragments from camel heavy-chain antibodies
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Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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9 September 2017
Naturally occurring antibodies devoid of light chains
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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9 September 2017
Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli.
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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9 September 2017
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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9 September 2017
A complex of nuclear pore proteins required for pore function
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PubMed Central
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9 September 2017
Comparison of in vivo and in vitro ribosomal RNA synthesis in nucleolar mutants ofXenopus laevis
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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9 September 2017
Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes.
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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30 September 2017
The C-terminal domain of Nup93 is essential for assembly of the structural backbone of nuclear pore complexes.
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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30 September 2017
The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry.
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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30 September 2017
Dual beneficial effect of interloop disulfide bond for single domain antibody fragments
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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30 September 2017
The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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28 June 2018
GraFix: sample preparation for single-particle electron cryomicroscopy
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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28 June 2018
Developmental changes in keratin patterns during epidermal maturation
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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28 June 2018
Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders.
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Site-specific biotinylation of purified proteins using BirA.
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Site-specific labeling of cysteine-tagged camelid single-domain antibody-fragments for use in molecular imaging
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Interaction of Nup53 with Ndc1 and Nup155 is required for nuclear pore complex assembly.
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution.
1 reference
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PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Microscopy and its focal switch.
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra.
1 reference
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PubMed Central
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4755751
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20 September 2018
iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification
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Crossref
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https://api.crossref.org/works/10.7554%2FELIFE.11349
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21 January 2018
Identifiers
DOI
10.7554/ELIFE.11349
1 reference
stated in
Europe PubMed Central
PMCID
4755751
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26633879%20AND%20SRC:MED&resulttype=core&format=json
retrieved
1 March 2020
PMCID
4755751
1 reference
stated in
Europe PubMed Central
PMCID
4755751
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26633879%20AND%20SRC:MED&resulttype=core&format=json
retrieved
1 March 2020
PubMed ID
26633879
1 reference
stated in
Europe PubMed Central
PMCID
4755751
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26633879%20AND%20SRC:MED&resulttype=core&format=json
retrieved
1 March 2020
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