(Q37698471)

22 August 2017

High-efficiency oligonucleotide-directed plasmid mutagenesis (English)

1 reference

22 August 2017

Olsen DB

1

1 reference

PMCID

53493

22 August 2017

Eckstein F

series ordinal

2

1 reference

PMCID

53493

22 August 2017

language of work or name

English

0 references

publication date

1 February 1990

1 reference

Proceedings of the National Academy of Sciences of the United States of America

22 August 2017

1 reference

22 August 2017

87

1 reference

22 August 2017

4

1 reference

22 August 2017

1451-1455

1 reference

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function

22 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

2 New M13 vectors for cloning

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Rapid and efficient site-specific mutagenesis without phenotypic selection

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Production of single-stranded plasmid DNA

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Construction of mutant and chimeric genes using the polymerase chain reaction

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Protein engineering. The design, synthesis and characterization of factitious proteins

23 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Tinkering with enzymes: what are we learning?

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

A simple method for site-directed mutagenesis using the polymerase chain reaction

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Directed mutagenesis using PCR.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Inhibition of restriction endonuclease hydrolysis by phosphorothioate-containing DNA.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Oligonucleotide-directed construction of mutations via gapped duplex DNA.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

A rapid and versatile site-directed method of mutagenesis for double-stranded plasmid DNA.

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

DNA filter retention assay for exonuclease activities. Application to the analysis of processivity of phage T5 induced 5'-exonuclease

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

Improved oligonucleotide-directed mutagenesis using M13 vectors

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

A simple and efficient method for the oligonucleotide-directed mutagenesis using plasmid DNA template and phosphorothioate-modified nucleotide

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=53493

A rapid method for site directed mutagenesis of plasmid DNA

23 June 2018

1 reference

12 December 2020

Cation Dependence of Restriction Endonuclease EcoRI Activity

1 reference

12 December 2020

Ethidium-bromide-inhibited restriction endonucleases cleave one strand of circular DNA

1 reference

12 December 2020

Rapid site-specific mutagenesis in plasmids

1 reference

12 December 2020

Use of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis

1 reference

12 December 2020

Identifiers

DOI

10.1073/PNAS.87.4.1451

1 reference

22 August 2017

0 references

1 reference

22 August 2017

1 reference

PMCID

53493