(Q38310245)

English

The use of site-directed mutagenesis, transient transfection, and radioligand binding. A method for the characterization of receptor-ligand interactions

scientific article published on January 2000

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scholarly article

1 reference

Europe PubMed Central

2 September 2017

The use of site-directed mutagenesis, transient transfection, and radioligand binding. A method for the characterization of receptor-ligand interactions (English)

1 reference

Europe PubMed Central

2 September 2017

author name string

J G Newell

1

1 reference

Europe PubMed Central

2 September 2017

M Davies

2

1 reference

Europe PubMed Central

2 September 2017

A N Bateson

3

1 reference

Europe PubMed Central

2 September 2017

publication date

1 January 2000

1 reference

Europe PubMed Central

2 September 2017

Molecular Biotechnology

1 reference

Europe PubMed Central

2 September 2017

14

1 reference

Europe PubMed Central

2 September 2017

1

1 reference

Europe PubMed Central

2 September 2017

25-45

1 reference

Europe PubMed Central

2 September 2017

https://scigraph.springernature.com/pub.10.1385/mb:14:1:25

0 references

In vitro mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The quantitative analysis of multiple mRNA species using oligonucleotide probes in an S1 nuclease protection assay

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Molecular biology of the GABA(A) receptor: functional domains implicated by mutational analysis

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Chemical and biochemical crosslinking of membrane components

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Addition of glycerol during DNA exposure enhances calcium phosphate transfection

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Receptor Binding Studies

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Principles of membrane protein assembly and structure

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid and efficient site-specific mutagenesis without phenotypic selection

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A modified two primer approach to oligonucleotide-directed in vitro mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Kinetics of [3H]muscimol binding to the GABAA receptor in bovine brain membranes

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis using double-stranded plasmid DNA templates

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis using positive antibiotic selection.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid and efficient site-specific mutagenesis without phenotypic selection

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Properties of γ-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Experimental strategies in efficient transfection of mammalian cells. Calcium phosphate and DEAE-dextran.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Mutagenesis at a specific position in a DNA sequence

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Oligonucleotide design and optimized protocol for site-directed mutagenesis.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Chemical mechanisms for photoaffinity labeling of receptors

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Efficient site-directed mutagenesis using uracil-containing DNA.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Structural requirements for ligand interactions at the benzodiazepine recognition site of the GABA(A) receptor

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Importance of DNA quality for transfection efficiency.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

High-efficiency transformation of mammalian cells by plasmid DNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A rapid-filtration technique for membrane fragments or immobilized enzymes: Measurements of substrate binding or ion fluxes with a few-millisecond time resolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A protocol for site-directed mutagenesis employing a uracil-containing phagemid template.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Efficient site-directed mutagenesis by simultaneous use of two primers

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis by the cassette method

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Efficient site directed in vitro mutagenesis using ampicillin selection

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A simple and efficient method for the oligodeoxyribonucleotide-directed mutagenesis of double-stranded plasmid DNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Crystallization of membrane proteins

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: II. In vitro selection of mutant DNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Multiple site-directed mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A simple assay for the study of solubilized acetylcholine receptors.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Photoaffinity labeling

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Molecular neurobiology of the GABAA receptor.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Characterization of γ‐Aminobutyric Acid‐Benzodiazepine Receptor Complexes by Protection Against Inactivation by Group‐Specific Reagents

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-specific mutagenesis in plasmids: a gapped circle method.

1 reference

https://pubmed.ncbi.nlm.nih.gov/10911613

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1385/MB:14:1:25

1 reference

Europe PubMed Central

2 September 2017

Dimensions Publication ID

0 references

1 reference

Europe PubMed Central

2 September 2017

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