(Q38343177)

English

Improved activity and modulated action pattern obtained by random mutagenesis at the fourth beta-alpha loop involved in substrate binding to the catalytic (beta/alpha)8-barrel domain of barley alpha-amylase 1.

scientific article published on September 1997

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scholarly article

1 reference

Europe PubMed Central

PubMed publication ID

2 September 2017

Improved activity and modulated action pattern obtained by random mutagenesis at the fourth beta-alpha loop involved in substrate binding to the catalytic (beta/alpha)8-barrel domain of barley alpha-amylase 1. (English)

1 reference

Europe PubMed Central

PubMed publication ID

2 September 2017

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author name string

Matsui I

1

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Europe PubMed Central

PubMed publication ID

2 September 2017

Svensson B

2

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Europe PubMed Central

PubMed publication ID

2 September 2017

language of work or name

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publication date

1 September 1997

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Europe PubMed Central

PubMed publication ID

2 September 2017

Journal of Biological Chemistry

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Europe PubMed Central

PubMed publication ID

2 September 2017

272

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Europe PubMed Central

PubMed publication ID

2 September 2017

36

1 reference

Europe PubMed Central

PubMed publication ID

2 September 2017

22456-22463

1 reference

Europe PubMed Central

PubMed publication ID

2 September 2017

Structure and possible catalytic residues of Taka-amylase A

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Calcium binding in alpha-amylases: an X-ray diffraction study at 2.1-A resolution of two enzymes from Aspergillus

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Crystal and molecular structure of barley alpha-amylase

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Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution

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The active center of a mammalian alpha-amylase. Structure of the complex of a pancreatic alpha-amylase with a carbohydrate inhibitor refined to 2.2-A resolution

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Roles of the aromatic residues conserved in the active center of Saccharomycopsis alpha-amylase for transglycosylation and hydrolysis activity.

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Expression of cDNAs encoding barley alpha-amylase 1 and 2 in yeast and characterization of the secreted proteins

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Domain B protruding at the third beta strand of the alpha/beta barrel in barley alpha-amylase confers distinct isozyme-specific properties.

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21 January 2018

An endogenous alpha-amylase inhibitor in barley kernels

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Barley malt-alpha-amylase. Purification, action pattern, and subsite mapping of isozyme 1 and two members of the isozyme 2 subfamily using p-nitrophenylated maltooligosaccharide substrates

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Subsite mapping of enzymes. Application of the depolymerase computer model to two alpha-amylases

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Subsite affinities of bacterial liquefying alpha-amylase evaluated from the rate parameters of linear substrates

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A Study of the Mechanism of Action of Taka-Amylase A1 on Linear Oligosaccharides by Product Analysis and Computer Simulation

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Subsite structure of Saccharomycopsis alpha-amylase secreted from Saccharomyces cerevisiae.

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Comparison of the domain-level organization of starch hydrolases and related enzymes

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A classification of glycosyl hydrolases based on amino acid sequence similarities

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Protein engineering in the alpha-amylase family: catalytic mechanism, substrate specificity, and stability

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Regional distant sequence homology between amylases, alpha-glucosidases and transglucanosylases.

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Parallel beta/alpha-barrels of alpha-amylase, cyclodextrin glycosyltransferase and oligo-1,6-glucosidase versus the barrel of beta-amylase: evolutionary distance is a reflection of unrelated sequences

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21 January 2018

Random mutagenesis used to probe the structure and function of Bacillus stearothermophilus alpha-amylase

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21 January 2018

RAmy2A; a novel alpha-amylase-encoding gene in rice

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21 January 2018

A mutant alpha-amylase with enhanced activity specific for short substrates

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21 January 2018

Site-directed mutagenesis of catalytic active-site residues of Taka-amylase A

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21 January 2018

Arginine is essential for the alpha-amylase inhibitory activity of the alpha-amylase/subtilisin inhibitor (BASI) from barley seeds

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Sequence diversity among related genes for recognition of specific targets in DNA molecules

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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

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C-terminal processing of barley alpha-amylase 1 in malt, aleurone protoplasts, and yeast

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris

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21 January 2018

A novel wheat alpha-amylase gene (alpha-Amy3).

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Identification of base mismatches recognized by the heteroduplex-DNA-repair system of Streptococcus pneumoniae

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Peptide YY receptors in the brain

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Substrate recognition at the binding site in mammalian pancreatic alpha-amylases

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Subsite mapping of enzymes. Studies on Bacillus subtilis amylase

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Structure and molecular model refinement of pig pancreatic alpha-amylase at 2.1 A resolution

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Starch- and glycogen-debranching and branching enzymes: prediction of structural features of the catalytic (beta/alpha)8-barrel domain and evolutionary relationship to other amylolytic enzymes

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Isolation of a cDNA Clone for alpha-Amylase in Mung Bean Cotyledons : Analysis of alpha-Amylase mRNA Levels in Cotyledons during and following Germination of Mung Bean Seeds

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Characterization, crystallization and preliminary X-ray crystallographic analysis of the complex between barley alpha-amylase and the bifunctional alpha-amylase/subtilisin inhibitor from barley seeds

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Alteration of bond-cleavage pattern in the hydrolysis catalyzed by Saccharomycopsis alpha-amylase altered by site-directed mutagenesis

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High-efficiency transformation of yeast by electroporation

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New substrate specificity of modified porcine pancreatic alpha-amylase

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Miniaturization of three carbohydrate analyses using a microsample plate reader

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Localization of an O-glycosylated site in the recombinant barley alpha-amylase 1 produced in yeast and correction of the amino acid sequence using matrix-assisted laser desorption/ionization mass spectrometry of peptide mixtures

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Multi-functional roles of a histidine residue in human pancreatic alpha-amylase

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Structure and expression of alpha-amylase gene from Vigna mungo

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Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions. Evaluation of subsite affinities of exo-enzyme

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Identifiers

10.1074/JBC.272.36.22456

1 reference

Europe PubMed Central

PubMed publication ID

2 September 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

2 September 2017

ResearchGate publication ID

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