(Q39136017)

9 September 2017

title

Use of an anti-apoptotic CHO cell line for transient gene expression (English)

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9 September 2017

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Nichole F Macaraeg

1

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9 September 2017

Dorothea E Reilly

series ordinal

2

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9 September 2017

Athena W Wong

series ordinal

3

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9 September 2017

publication date

22 June 2013

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9 September 2017

published in

Biotechnology Progress

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9 September 2017

volume

29

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9 September 2017

issue

4

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9 September 2017

page(s)

1050-1058

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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

9 September 2017

cites work

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Recombinant protein production by transient gene transfer into Mammalian cells

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100-liter transient transfection

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A simple high-yielding process for transient gene expression in CHO cells.

21 January 2018

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Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors

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CHO cells in biotechnology for production of recombinant proteins: current state and further potential

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Animal-cell damage in sparged bioreactors

21 January 2018

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Application of hypoosmolar medium to fed-batch culture of hybridoma cells for improvement of culture longevity

21 January 2018

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Mitochondria and cell death: outer membrane permeabilization and beyond

21 January 2018

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The Bcl2 family: regulators of the cellular life-or-death switch

21 January 2018

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RNAi suppression of Bax and Bak enhances viability in fed-batch cultures of CHO cells

21 January 2018

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BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

21 January 2018

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DNA internalized via caveolae requires microtubule-dependent, Rab7-independent transport to the late endocytic pathway for delivery to the nucleus

21 January 2018

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Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection

21 January 2018

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Transfection efficiency and uptake process of polyplexes in human lung endothelial cells: a comparative study in non-polarized and polarized cells.

21 January 2018

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Serum-free large-scale transient transfection of CHO cells

21 January 2018

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The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells

21 January 2018

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Biophysical characterization of PEI/DNA complexes

21 January 2018

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Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture

21 January 2018

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Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture

21 January 2018

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New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

21 January 2018

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Molecular mechanisms of caspase regulation during apoptosis

21 January 2018

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Caspases: the executioners of apoptosis

21 January 2018

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Poly(ethylenimine) and its role in gene delivery

21 January 2018

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Opposing roles of syndecan-1 and syndecan-2 in polyethyleneimine-mediated gene delivery

21 January 2018

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A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin.

21 January 2018

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Extracellular glycosaminoglycans modify cellular trafficking of lipoplexes and polyplexes

21 January 2018

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Enhancement of DNA uptake in FUT8-deleted CHO cells for transient production of afucosylated antibodies

21 January 2018

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Gene delivery by cationic lipid vectors: overcoming cellular barriers

21 January 2018

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Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery

21 January 2018

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Regulated portals of entry into the cell

21 January 2018

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Pathways of clathrin-independent endocytosis

21 January 2018

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PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process

21 January 2018

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N/P ratio significantly influences the transfection efficiency and cytotoxicity of a polyethylenimine/chitosan/DNA complex

21 January 2018

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Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production

21 January 2018

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Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/BTPR.1763

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9 September 2017

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