(Q39663162)

13 September 2017

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title

Hybridoma technology: new developments of practical interest (English)

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13 September 2017

Samoilovich SR

1

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13 September 2017

Dugan CB

series ordinal

2

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13 September 2017

Macario AJ

series ordinal

3

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13 September 2017

publication date

1 August 1987

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Journal of Immunological Methods

13 September 2017

published in

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13 September 2017

volume

101

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13 September 2017

issue

2

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13 September 2017

page(s)

153-170

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Continuous cultures of fused cells secreting antibody of predefined specificity

13 September 2017

cites work

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12 December 2020

Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient.

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12 December 2020

Improved fusion methods. IV. Technical aspects

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12 December 2020

Human and chimpanzee monoclonal antibodies

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12 December 2020

The use of Epstein-Barr virus transformation for the production of human monoclonal antibodies

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12 December 2020

Monoclonal antibody production by receptor-mediated electrically induced cell fusion

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12 December 2020

Human hybridoma cells produced by electro-fusion

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12 December 2020

Cloned mycoplasma ribosomal RNA genes for the detection of mycoplasma contamination in tissue cultures

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12 December 2020

Rat monoclonal antibodies. III. A simple method for facilitation of hybridoma cell growth in vivo

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12 December 2020

Serum-free medium for hybridoma and parental myeloma cell cultivation: a novel composition of growth-supporting substances

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12 December 2020

Specific immunoglobulin production and enhanced tumorigenicity following ascites growth of human hybridomas

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12 December 2020

Theory and methods for immunization in culture and monoclonal antibody production

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12 December 2020

Antibody engineering, a strategy for the development of monoclonal antibodies

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12 December 2020

UC 729-6, a human lymphoblastoid B-cell line useful for generating antibody-secreting human-human hybridomas

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12 December 2020

Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production

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12 December 2020

Production of an ovine monoclonal antibody to testosterone by an interspecies fusion

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12 December 2020

Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum

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12 December 2020

Derivation and diversification of monoclonal antibodies

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12 December 2020

Antibody production by human × human hybridomas in serum-free medium

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12 December 2020

Hybridoma growth and antibody secretion in serum-supplemented and low protein serum-free media

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12 December 2020

Human monoclonal antibodies

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12 December 2020

The production of monoclonal antibodies from human lymphocytes

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12 December 2020

In vitro stimulated lymphocytes as a source of human hybridomas

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12 December 2020

In vitro immunization of human lymphocytes. I. Production of human monoclonal antibodies against bombesin and tetanus toxoid.

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12 December 2020

A porcine-murine hybridoma that secretes porcine monoclonal antibody of defined specificity

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12 December 2020

Entrapment of animal cells for production of monoclonal antibodies and other biomolecules

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12 December 2020

The production of a monoclonal antibody that blocks the action of a neurite outgrowth-promoting factor

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12 December 2020

The large-scale cultivation of mammalian cells

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12 December 2020

HO-323, a human B-lymphoblastoid cell line useful for making human-human hybridomas

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12 December 2020

Growth of rat-mouse hybridomas in nude mice and nude rats

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12 December 2020

In vitro stimulation prior to fusion generates antigen-binding human-human hybridomas

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12 December 2020

Passive immunization: a method of enhancing the immune response against antigen mixtures.

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12 December 2020

Antigen-promoted cell fusion: antigen-coated myeloma cell fuse with antigen-reactive spleen cells

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12 December 2020

Human hybridomas constructed with antigen-specific Epstein-Barr virus-transformed cell lines

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12 December 2020

Long-term antibody synthesis in vitro. V. Conditions for prolonging the response, selection of different cell types and interculture transfer of functional structures

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12 December 2020

Specificity of anti-polynucleotide monoclonal antibodies from human-human hybridomas

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12 December 2020

Human monoclonals from antigen-specific selection of B lymphocytes and transformation by EBV.

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12 December 2020

Conditioned medium from macrophage cell lines supports the single-cell growth of hybridomas

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12 December 2020

Hybridomas from unimmunized individuals

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12 December 2020

Modified hybridoma methodology: antigen-directed chemically mediated cell fusion

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12 December 2020

Monoclonal Antibodies Reveal the Structural Basis of Antibody Diversity

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12 December 2020

Production of monoclonal antibodies. The effect of hybridoma concentration on the yield of antibody-producing clones

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12 December 2020

Advantages of rat monoclonal antibodies.

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12 December 2020

Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma.

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12 December 2020

Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion

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12 December 2020

Studies on the mechanism of polyethylene glycol-mediated cell fusion using fluorescent membrane and cytoplasmic probes

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12 December 2020

An immunoenzymatic system to study in vitro immune responses. Synthesis of antibodies directed toward different -D-galactosidase determinants in lymph node fragment cultures.

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12 December 2020

Production of monoclonal antibodies: strategy and tactics

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12 December 2020

Novel approach to construction of human "myeloma analogues" for production of human monoclonal antibodies

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12 December 2020

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

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12 December 2020

Human X (mouse X human) hybridomas stably producing human antibodies

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12 December 2020

Influence of fusion cell ratio and cell plating density on production of human-human hybridomas secreting anti-DNA autoantibodies from patients with systemic lupus erythematosus

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12 December 2020

The efficient production of stable, human monoclonal antibody-secreting hybridomas from EBV-transformed lymphocytes using the mouse myeloma X63-Ag8.653 as a fusion partner.

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12 December 2020

A comparative analysis of the phenotypic characteristics of available fusion partners for the construction of human hybridomas

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12 December 2020

Production of human monoclonal antibody in mouse ascites

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12 December 2020

Detection of mycoplasmas infecting cell cultures by DNA hybridization

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12 December 2020

A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro

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12 December 2020

Production of a human monoclonal antibody to HLA by human-human hybridoma technology. A preliminary report.

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12 December 2020

Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins

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12 December 2020

Kinetics of monoclonal antibody production in low serum growth medium

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12 December 2020

Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG

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12 December 2020

Human hybridomas secreting anti-islet autoantibodies

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12 December 2020

Routine large-scale production of monoclonal antibodies in a protein-free culture medium

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12 December 2020

Increase of hybridoma formation by addition of dextran sulphate to in vitro immunization system

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12 December 2020

Production of IgG-producing hybridomas by in vitro stimulation of murine spleen cells

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12 December 2020

Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line

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12 December 2020

Human-human B cell hybridomas from in vitro stimulated lymphocytes of patients with common variable immunodeficiency

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12 December 2020

Production of human hybridomas secreting antibodies to measles virus

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12 December 2020

Rat monoclonal antibodies. IV. Easy method for in vitro production

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12 December 2020

Colcemid treatment of myeloma prior to cell fusion increases the yield of hybridomas between myeloma and splenocyte

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12 December 2020

Production of primate monoclonal antibodies

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12 December 2020

Long-term antibody synthesis in vitro. VII. Induction of immunologic memory in cultures of horse lymph nodes

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12 December 2020

Comparison of polyethylene glycols as fusogens for producing lymphocyte-myeloma hybrids

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12 December 2020

Antigenic mosaic of Methanosarcinaceae: partial characterization of Methanosarcina barkeri 227 surface antigens by monoclonal antibodies

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12 December 2020

A human-human hybridoma secreting anti-HLA class II antibody

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12 December 2020

Determination of the optimal human cell lines for development of human hybridomas

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12 December 2020

Methods for the production of xenogeneic monoclonal antibodies in murine ascites

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12 December 2020

A better cell line for making hybridomas secreting specific antibodies

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12 December 2020

Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity

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12 December 2020

Reproducible production of protective human monoclonal antibodies by fusion of peripheral blood lymphocytes with a mouse myeloma cell line

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12 December 2020

The WI-L2-729-HF2 human hybridoma system. Stable hybrids at high frequency

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12 December 2020

A human-hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line

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12 December 2020

Proteins transferred to nitrocellulose for use as immunogens

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12 December 2020

Optimization of conditions for in vitro antigenic stimulation of dissociated mouse spleen cells for the production of monoclonal antibodies against peptide hormones

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12 December 2020

Large-scale production of monoclonal antibodies in dialysis tubing

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12 December 2020

Continuous hybridoma growth and monoclonal antibody production in hollow fiber reactors-separators

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12 December 2020

Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen

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12 December 2020

Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas

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12 December 2020

Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas

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12 December 2020

Identification and characterization of rabbit-mouse hybridomas secreting rabbit immunoglobulin chains

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12 December 2020

Production of monoclonal antibodies in large-scale cell culture

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12 December 2020

Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual

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12 December 2020

Production of stable mouse x human hybridomas secreting HBs antigen-specific human monoclonal antibody by using in vitro sensitization

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12 December 2020

Methods of enhancing the frequency of antigen-specific hybridomas

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12 December 2020

Factors affecting cell growth and monoclonal antibody production in stirred reactors

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12 December 2020

Stable antibody-producing murine hybridomas

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12 December 2020

High frequency fusion of plant protoplasts by electric fields

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12 December 2020

Bovine-murine hybridoma that secretes bovine monoclonal antibody of defined specificity

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12 December 2020

Hybridoma production by simplified avidin-mediated electrofusion

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12 December 2020

Entrapment of animal cells for the production of biomolecules such as monoclonal antibodies

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12 December 2020

Tailor-made monoclonal antibodies

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12 December 2020

10.1016/0022-1759(87)90147-5

Identifiers

DOI

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13 September 2017

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