(Q40420420)

20 September 2017

title

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production (English)

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20 September 2017

Lucia Baldi

1

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20 September 2017

Natalie Muller

2

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20 September 2017

Sylviane Picasso

3

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20 September 2017

Raymond Jacquet

4

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20 September 2017

Philippe Girard

5

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20 September 2017

Huy Phan Thanh

6

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20 September 2017

Elisabeth Derow

7

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20 September 2017

Florian M Wurm

8

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20 September 2017

publication date

1 January 2005

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20 September 2017

published in

Biotechnology Progress

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20 September 2017

volume

21

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20 September 2017

issue

1

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20 September 2017

page(s)

148-153

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Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells

20 September 2017

cites work

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trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1.

21 January 2018

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Industrial choices for protein production by large-scale cell culture

21 January 2018

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7 January 2021

Biochemical engineering: Bioprocessing of therapeutic proteins

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7 January 2021

Large-scale transient expression in mammalian cells for recombinant protein production

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7 January 2021

Versatile expression system for rapid and stable production of recombinant proteins

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7 January 2021

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

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7 January 2021

Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

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7 January 2021

Transient gene expression in mammalian cells grown in serum-free suspension culture

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7 January 2021

Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: peptone additives improve cell growth and transfection efficiency

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7 January 2021

Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: control of physicochemical parameters allows transfection in stirred media. Transfection and protein expression in mammalian cells

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7 January 2021

100-liter transient transfection

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7 January 2021

Serum-free large-scale transient transfection of CHO cells

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7 January 2021

Calcium phosphate transfection optimization for serum-free suspension culture

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7 January 2021

Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis

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7 January 2021

Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example

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7 January 2021

Small-scale bioreactor system for process development and optimization

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7 January 2021

CHO expression of a novel human recombinant IgG1 anti-RhD antibody isolated by phage display

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7 January 2021

Differential efficiency of expression of humanized antibodies in transient transfected mammalian cells

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7 January 2021

A single amino acid substitution in the variable region of the light chain specifically blocks immunoglobulin secretion

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7 January 2021

Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria

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7 January 2021

Association of Human Protein-tyrosine Phosphatase κ with Members of the Armadillo Family

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7 January 2021

Calsyntenin-1, a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain

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7 January 2021

Soluble dimeric prion protein binds PrP(Sc) in vivo and antagonizes prion disease

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7 January 2021

Protein expression in mammalian and insect cell systems

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7 January 2021

A new technique for the assay of infectivity of human adenovirus 5 DNA

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7 January 2021

Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation

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7 January 2021

Calfection: a novel gene transfer method for suspension cells.

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7 January 2021

Effects of pCIneo and pCEP4 expression vectors on transient and stable protein production in human and simian cell lines

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7 January 2021

Mutagenesis of a potential immunoglobulin-binding protein-binding site enhances secretion of coagulation factor VIII

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7 January 2021

Identifiers

DOI

10.1021/BP049830X

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20 September 2017

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