(Q40648473)

22 September 2017

title

RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression (English)

1 reference

22 September 2017

8

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7 June 2020

Xiaoying Bian

6

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7 June 2020

author name string

Hailong Wang

1

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7 June 2020

Zhen Li

2

1 reference

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7 June 2020

Ruonan Jia

3

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7 June 2020

Yu Hou

4

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7 June 2020

Jia Yin

5

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7 June 2020

Aiying Li

7

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7 June 2020

A Francis Stewart

9

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7 June 2020

Jun Fu

10

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7 June 2020

Youming Zhang

11

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7 June 2020

publication date

2 June 2016

1 reference

22 September 2017

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7 June 2020

volume

11

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7 June 2020

issue

7

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7 June 2020

page(s)

1175-1190

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Microbial genomics for the improvement of natural product discovery

7 June 2020

cites work

1 reference

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21 January 2018

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Discovery of a new peptide natural product by Streptomyces coelicolor genome mining

21 January 2018

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Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans

21 January 2018

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The impact of genomics on the exploitation of the myxobacterial secondary metabolome

21 January 2018

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Metagenomic small molecule discovery methods

21 January 2018

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Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.

21 January 2018

1 reference

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Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.

21 January 2018

1 reference

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Heterologous expression of an orphan NRPS gene cluster from Paenibacillus larvae in Escherichia coli revealed production of sevadicin

21 January 2018

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Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in Aspergillus nidulans

21 January 2018

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Assembly and heterologous expression of the coumermycin A1 gene cluster and production of new derivatives by genetic engineering

21 January 2018

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Heterologous expression and genetic engineering of the tubulysin biosynthetic gene cluster using Red/ET recombineering and inactivation mutagenesis

21 January 2018

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Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition

21 January 2018

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Reconstitution of the myxothiazol biosynthetic gene cluster by Red/ET recombination and heterologous expression in Myxococcus xanthus

21 January 2018

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Heterologous expression of a myxobacterial natural products assembly line in pseudomonads via red/ET recombineering.

21 January 2018

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Improved seamless mutagenesis by recombineering using ccdB for counterselection

21 January 2018

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A new logic for DNA engineering using recombination in Escherichia coli

21 January 2018

1 reference

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Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through Red/ET recombineering.

21 January 2018

1 reference

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Heterologous production of glidobactins/luminmycins in Escherichia coli Nissle containing the glidobactin biosynthetic gene cluster from Burkholderia DSM7029.

21 January 2018

1 reference

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In vivo evidence for a prodrug activation mechanism during colibactin maturation.

21 January 2018

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DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

21 January 2018

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Enzymatic assembly of DNA molecules up to several hundred kilobases

21 January 2018

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Rapid modification of bacterial artificial chromosomes by ET-recombination.

21 January 2018

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Point mutation of bacterial artificial chromosomes by ET recombination

21 January 2018

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Heterologous expression of novobiocin and clorobiocin biosynthetic gene clusters

21 January 2018

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Heterologous expression and genetic engineering of the phenalinolactone biosynthetic gene cluster by using red/ET recombineering

21 January 2018

1 reference

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High-titer heterologous production in E. coli of lyngbyatoxin, a protein kinase C activator from an uncultured marine cyanobacterium

21 January 2018

1 reference

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Elucidating the mechanism of cis double bond formation in epothilone biosynthesis.

21 January 2018

1 reference

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Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo.

21 January 2018

1 reference

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Genetically engineered lipopeptide antibiotics related to A54145 and daptomycin with improved properties

21 January 2018

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Combinatorial biosynthesis of novel antibiotics related to daptomycin

21 January 2018

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Development of a Genetic System for Combinatorial Biosynthesis of Lipopeptides in Streptomyces fradiae and Heterologous Expression of the A54145 Biosynthesis Gene Cluster

21 January 2018

1 reference

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Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2).

21 January 2018

1 reference

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An improved recombineering approach by adding RecA to lambda Red recombination

21 January 2018

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A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.

21 January 2018

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Positive and negative roles of an initiator protein at an origin of replication

21 January 2018

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Advanced genetic strategies for recombinant protein expression in Escherichia coli

21 January 2018

1 reference

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Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

21 January 2018

1 reference

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A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites.

21 January 2018

1 reference

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Interactions of the origin of replication (oriV) and initiation proteins (TrfA) of plasmid RK2 with submembrane domains of Escherichia coli

21 January 2018

1 reference

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Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting.

21 January 2018

1 reference

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Broad host range vectors for stable genomic library construction

21 January 2018

1 reference

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Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms.

21 January 2018

1 reference

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Development of a broad-host synthetic biology toolbox for Ralstonia eutropha and its application to engineering hydrocarbon biofuel production

21 January 2018

1 reference

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DNA cloning by homologous recombination in Escherichia coli

21 January 2018

1 reference

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Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

31 March 2020

1 reference

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31 March 2020

Identifiers

DOI

10.1038/NPROT.2016.054

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:27254463%20AND%20SRC:MED&resulttype=core&format=json

7 June 2020

1 reference