(Q41825705)

English

Overproduction of the pyruvate dehydrogenase multienzyme complex of Escherichia coli and site-directed substitutions in the E1p and E2p subunits

scientific article published on October 1992

In more languages

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scholarly article

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

Overproduction of the pyruvate dehydrogenase multienzyme complex of Escherichia coli and site-directed substitutions in the E1p and E2p subunits (English)

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

Escherichia coli

0 references

author name string

Russell GC

1

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

Machado RS

2

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

Guest JR

3

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

language of work or name

0 references

publication date

1 October 1992

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

Biochemical Journal

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

287 ( Pt 2)

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

611-619

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

Protein measurement with the Folin phenol reagent

1 reference

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6 June 2018

Transduction of linked genetic characters of the host by bacteriophage P1.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1 reference

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6 June 2018

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1 reference

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6 June 2018

Rapid and efficient site-specific mutagenesis without phenotypic selection

1 reference

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6 June 2018

Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.

1 reference

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6 June 2018

Double stranded DNA sequencing as a choice for DNA sequencing

1 reference

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6 June 2018

Segmental structure and protein domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. Genetic reconstruction in vitro and 1H-n.m.r. spectroscopy

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Limited proteolysis and sequence analysis of the 2-oxo acid dehydrogenase complexes from Escherichia coli. Cleavage sites and domains in the dihydrolipoamide acyltransferase components

1 reference

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6 June 2018

Structure of chloramphenicol acetyltransferase at 1.75-A resolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Site-directed mutagenesis and 1H NMR spectroscopy of an interdomain segment in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Subgenes expressing single lipoyl domains of the pyruvate dehydrogenase complex of Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Investigation of the mechanism of active site coupling in the pyruvate dehydrogenase multienzyme complex of Escherichia coli by protein engineering.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Ptac-85, an E. coli vector for expression of non-fusion proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Homologous plant and bacterial proteins chaperone oligomeric protein assembly

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

A common structural motif in thiamin pyrophosphate-binding enzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Structure, expression, and protein engineering of the pyruvate dehydrogenase complex of Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Overexpression and mutagenesis of the catalytic domain of dihydrolipoamide acetyltransferase from Saccharomyces cerevisiae

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Overexpression of restructured pyruvate dehydrogenase complexes and site-directed mutagenesis of a potential active-site histidine residue

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Genetic reconstruction and characterization of the recombinant transacylase (E2b) component of bovine branched-chain alpha-keto acid dehydrogenase complex. Implication of histidine 391 as an active site residue

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75 A resolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Isolation and characterization of lipoylated and unlipoylated domains of the E2p subunit of the pyruvate dehydrogenase complex of Escherichia coli

1 reference

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6 June 2018

Structure and regulation of KGD2, the structural gene for yeast dihydrolipoyl transsuccinylase

1 reference

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6 June 2018

Evidence for transition-state stabilization by serine-148 in the catalytic mechanism of chloramphenicol acetyltransferase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

Sequence conservation in the alpha and beta subunits of pyruvate dehydrogenase and its similarity to branched-chain alpha-keto acid dehydrogenase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1133209

6 June 2018

The catalytic domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii and Escherichia coli. Expression, purification, properties and preliminary X-ray analysis.

1 reference

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Domains, motifs, and linkers in 2-oxo acid dehydrogenase multienzyme complexes: a paradigm in the design of a multifunctional protein

1 reference

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6 June 2018

Lipoylation of the E2 components of the 2-oxo acid dehydrogenase multienzyme complexes of Escherichia coli

1 reference

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6 June 2018

Sequence similarities within the family of dihydrolipoamide acyltransferases and discovery of a previously unidentified fungal enzyme

1 reference

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6 June 2018

Effects of substitution of aspartate-440 and tryptophan-487 in the thiamin diphosphate binding region of pyruvate decarboxylase from Zymomonas mobilis.

1 reference

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6 June 2018

Secretory S complex of Bacillus subtilis: sequence analysis and identity to pyruvate dehydrogenase

1 reference

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6 June 2018

Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex

1 reference

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6 June 2018

Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH

1 reference

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6 June 2018

Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex of Escherichia coli

1 reference

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6 June 2018

Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12

1 reference

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The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide sequence encoding the dihydrolipoamide acetyltransferase component.

1 reference

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18 August 2018

The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Pyruvate Dehydrogenase Component

1 reference

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Evidence for two protein-lipoylation activities inEscherichia coli

1 reference

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18 August 2018

Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis of the lipoate acetyltransferase of Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Two lipoyl domains in the dihydrolipoamide acetyltransferase chain of the pyruvate dehydrogenase multienzyme complex of Streptococcus faecalis

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The alpha-ketoacid dehydrogenase complexes. Sequence similarity of rat pyruvate dehydrogenase with Escherichia coli and Azotobacter vinelandii alpha-ketoglutarate dehydrogenase

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Chain folding in the dihydrolipoyl acyltransferase components of the 2-oxo-acid dehydrogenase complexes from Escherichia coli. Identification of a segment involved in binding the E3 subunit

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/1445221

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ2870611

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

12 October 2017

ResearchGate publication ID

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